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European Spine Journal
Erschienen in: European Spine Journal 11/2009

01.11.2009 | Review Article

Nucleus pulposus tissue engineering: a brief review

verfasst von: Xinlin Yang, Xudong Li

Erschienen in: European Spine Journal | Ausgabe 11/2009

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Abstract

Symptomatic intervertebral disc degeneration is associated with several spinal diseases, which cause losses of life quality and money. Tissue engineering provides a promising approach to recover the functionality of the degenerative intervertebral disc. Most studies are directed toward nucleus pulposus (NP) tissue engineering because disc degeneration is believed to originate in NP region, and considerable progress has been made in the past decade. Before this important technique is utilized for clinical treatment of disc degeneration, many challenges need to address including in all three principal components of tissue engineering, i.e., seed cells, signals and biomaterial scaffolds. This article briefly gives certain aspects of state of the art in this field, as well as pays a little more attention to our work published in the past 5 years, on growth and differentiation factor-5 (GDF-5), adipose-derived stem cells (ADSCs) and heparin functionalization of scaffold. We suggest that combinatorial application of ADSCs, GDF-5, heparin functionalization and injectable hydrogels will be advantageous in NP tissue engineering.
Literatur
1.
Adams MA, Freeman BJ, Morrison HP, Nelson IW, Dolan P (2000) Mechanical initiation of intervertebral disc degeneration. Spine 25:1625–1636CrossRefPubMed
2.
Bader RA, Rochefort WE (2008) Rheological characterization of photopolymerized poly(vinyl alcohol) hydrogels for potential use in nucleus pulposus replacement. J Biomed Mater Res A. 86:494–501PubMed
3.
Benoit DS, Collins SD, Anseth KS (2007) Multifunctional hydrogels that promote osteogenic hMSC differentiation through stimulation and sequestering of BMP2. Adv Funct Mater 17:2085–2093CrossRefPubMed
4.
Bibby SR, Urban JP (2004) Effect of nutrient deprivation on the viability of intervertebral disc cells. Eur Spine J 13:695–701CrossRefPubMed
5.
Boyd LM, Carter AJ (2006) Injectable biomaterials and vertebral endplate treatment for repair and regeneration of the intervertebral disc. Eur Spine J 15(Suppl 3):S414–S421CrossRefPubMed
6.
Chen FH, Tuan RS (2008) Mesenchymal stem cells in arthritic diseases. Arthr Res Ther 10:223CrossRef
7.
Cho J, Heuzey MC, Bégin A, Carreau PJ (2005) Physical gelation of chitosan in the presence of beta-glycerophosphate: the effect of temperature. Biomacromolecules 6:3267–3275CrossRefPubMed
8.
Chou AI, Nicoll SB (2008) Characterization of photocrosslinked alginate hydrogels for nucleus pulposus cell encapsulation. J Biomed Mater Res A [Epub ahead of print]
9.
Chujo T, An HS, Akeda K, Miyamoto K, Muehleman C, Attawia M, Andersson G, Masuda K (2006) Effects of growth differentiation factor-5 on the intervertebral disc—in vitro bovine study and in vivo rabbit disc degeneration model study. Spine 31:2909–2917CrossRefPubMed
10.
Cui M, Wan Y, Anderson DG, Shen FH, Leo BM, Laurencin CT, Balian G, Li X (2008) Mouse growth and differentiation factor-5 protein and DNA therapy potentiates intervertebral disc cell aggregation and chondrogenic gene expression. Spine J 8:287–295CrossRefPubMed
11.
Di Martino A, Sittinger M, Risbud MV (2005) Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 26:5983–5990CrossRefPubMed
12.
Edlund U, Dånmark S, Albertsson AC (2008) A strategy for the covalent functionalization of resorbable polymers with heparin and osteoinductive growth factor. Biomacromolecules 9:901–905CrossRefPubMed
13.
Feng G, Wan Y, Balian G, Laurencin CT, Li X (2008) Adenovirus-mediated expression of growth and differentiation factor-5 promotes chondrogenesis of adipose stem cells. Growth Factors 26:132–142CrossRefPubMed
14.
Freemont AJ (2009) The cellular pathobiology of the degenerate intervertebral disc and discogenic back pain. Rheumatology (Oxford) 48:5–10CrossRef
15.
Friedenstein AJ, Piatetzky-Shapiro II, Petrakova KV (1966) Osteogenesis in transplants of bone marrow cells. J Embryol Exp Morphol 16:381-390
16.
Fujita M, Ishihara M, Simizu M, Obara K, Ishizuka T, Saito Y, Yura H, Morimoto Y, Takase B, Matsui T, Kikuchi M, Maehara T (2004) Vascularization in vivo caused by the controlled release of fibroblast growth factor-2 from an injectable chitosan/non-anticoagulant heparin hydrogel. Biomaterials 25:699–706CrossRefPubMed
17.
Fujita N, Miyamoto T, Imai J, Hosogane N, Suzuki T, Yagi M, Morita K, Ninomiya K, Miyamoto K, Takaishi H, Matsumoto M, Morioka H, Yabe H, Chiba K, Watanabe S, Toyama Y, Suda T (2005) CD24 is expressed specifically in the nucleus pulposus of intervertebral discs. Biochem Biophys Res Commun 338:1890–1896CrossRefPubMed
18.
Furue MK, Na J, Jackson JP, Okamoto T, Jones M, Baker D, Hata R, Moore HD, Sato JD, Andrews PW (2008) Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium. Proc Natl Acad Sci USA 105:13409–13414CrossRefPubMed
19.
Gaetani P, Torre ML, Klinger M, Faustini M, Crovato F, Bucco M, Marazzi M, Chlapanidas T, Levi D, Tancioni F, Vigo D, Rodriguez y Baena R (2008) Adipose-derived stem cell therapy for intervertebral disc regeneration: an in vitro reconstructed tissue in alginate capsules. Tissue Eng Part A 14:1415–1423CrossRefPubMed
20.
Gilbertson L, Ahn SH, Teng PN, Studer RK, Niyibizi C, Kang JD (2008) The effects of recombinant human bone morphogenetic protein-2, recombinant human bone morphogenetic protein-12, and adenoviral bone morphogenetic protein-12 on matrix synthesis in human annulus fibrosis and nucleus pulposus cells. Spine J 8:449–456CrossRefPubMed
21.
Gruber HE, Norton HJ, Hanley EN Jr (2000) Anti-apoptotic effects of IGF-1 and PDGF on human intervertebral disc cells in vitro. Spine 25:2153–2157CrossRefPubMed
22.
Haefeli M, Kalberer F, Saegesser D, Nerlich AG, Boos N, Paesold G (2006) The course of macroscopic degeneration in the human lumbar intervertebral disc. Spine 31:1522–1531CrossRefPubMed
23.
Hiyama A, Mochida J, Iwashina T, Omi H, Watanabe T, Serigano K, Tamura F, Sakai D (2008) Transplantation of mesenchymal stem cells in a canine disc degeneration model. J Orthop Res 26:589–600CrossRefPubMed
24.
Huang KY, Yan JJ, Hsieh CC, Chang MS, Lin RM (2007) The in vivo biological effects of intradiscal recombinant human bone morphogenetic protein-2 on the injured intervertebral disc: an animal experiment. Spine 32:1174–1180CrossRefPubMed
25.
Jandial R, Aryan HE, Park J, Taylor WT, Snyder EY (2008) Stem cell-mediated regeneration of the intervertebral disc: cellular and molecular challenge. Neurosurg Focus 24:E21CrossRefPubMed
26.
Joung YK, Bae JW, Park KD (2008) Controlled release of heparin-binding growth factors using heparin-containing particulate systems for tissue regeneration. Expert Opin Drug Deliv 5:1173–1184CrossRefPubMed
27.
Kandel R, Roberts S, Urban JP (2008) Tissue engineering and the intervertebral disc: the challenges. Eur Spine J 17(Suppl 4):480–491CrossRefPubMed
28.
Kawakami M, Matsumoto T, Hashizume H, Kuribayashi K, Chubinskaya S, Yoshida M (2005) Osteogenic protein-1 (osteogenic protein-1/bone morphogenetic protein-7) inhibits degeneration and pain-related behavior induced by chronically compressed nucleus pulposus in the rat. Spine 30:1933–1939CrossRefPubMed
29.
Lee CR, Sakai D, Nakai T, Toyama K, Mochida J, Alini M, Grad S (2007) A phenotypic comparison of intervertebral disc and articular cartilage cells in the rat. Eur Spine J 16:2174–2185CrossRefPubMed
30.
Li J, Zhu B, Shao Y, Liu X, Yang X, Yu Q (2009) Construction of anticoagulant poly (lactic acid) films via surface covalent graft of heparin-carrying microcapsules. Colloids Surf B Biointerfaces 70:15–19CrossRefPubMed
31.
Li X, Leo BM, Beck G, Balian G, Anderson GD (2004) Collagen and proteoglycan abnormalities in the GDF-5-deficient mice and molecular changes when treating disk cells with recombinant growth factor. Spine 29:2229–2234CrossRefPubMed
32.
Li X, Lee JP, Balian G, Greg Anderson D (2005) Modulation of chondrocytic properties of fat-derived mesenchymal cells in co-cultures with nucleus pulposus. Connect Tissue Res 46:75–82CrossRefPubMed
33.
Lu ZF, Doulabi BZ, Wuisman PI, Bank RA, Helder MN (2007) Differentiation of adipose stem cells by nucleus pulposus cells: configuration effect. Biochem Biophys Res Commun 359:991–996CrossRefPubMed
34.
Lu ZF, Doulabi BZ, Wuisman PI, Bank RA, Helder MN (2008) Influence of collagen type II and nucleus pulposus cells on aggregation and differentiation of adipose tissue-derived stem cells. J Cell Mol Med 12:2812–2822CrossRefPubMed
35.
Luo X, Qiu D, He B, Wang LJ, Luo J (2006) Biodegradable heparin-loaded microspheres: carrier molecular composition and microsphere structure. Macromol Biosci 6:373–381CrossRefPubMed
36.
Masuda K, Imai Y, Okuma M, Muehleman C, Nakagawa K, Akeda K, Thonar E, Andersson G, An HS (2006) Osteogenic protein-1 injection into a degenerated disc induces the restoration of disc height and structural changes in the rabbit anular puncture model. Spine 31:742–754CrossRefPubMed
37.
Matsunaga S, Nagano S, Onishi T, Morimoto N, Suzuki S, Komiya S (2003) Age-related changes in expression of transforming growth factor-beta and receptors in cells of intervertebral discs. J Neurosurg 98:63–67PubMed
38.
Murugesan S, Xie J, Linhardt RJ (2008) Immobilization of heparin: approaches and applications. Curr Top Med Chem 8:80–100CrossRefPubMed
39.
Mwale F, Roughley P, Antoniou J (2004) Distinction between the extracellular matrix of the nucleus pulposus and hyaline cartilage: a requisite for tissue engineering of intervertebral disc. Eur Cell Mater 8:58–63PubMed
40.
Nair LS, Starnes T, Ko JW, Laurencin CT (2007) Development of injectable thermogelling chitosan-inorganic phosphate solutions for biomedical applications. Biomacromolecules 8:3779–3785CrossRefPubMed
41.
Nie H, Wang CH (2007) Fabrication and characterization of PLGA/HAp composite scaffolds for delivery of BMP-2 plasmid DNA. J Control Release 120:111–121CrossRefPubMed
42.
Nie T, Akins RE Jr, Kiick KL (2009) Production of heparin-containing hydrogels for modulating cell responses. Acta Biomater 5:865–875CrossRefPubMed
43.
Nomura T, Mochida J, Okuma M, Nishimura K, Sakabe K (2001) Nucleus pulposus allograft retards intervertebral disc degeneration. Clin Orthop Relat Res 389:94–101CrossRefPubMed
44.
O’Halloran DM, Pandit AS (2007) Tissue-engineering approach to regenerating the intervertebral disc. Tissue Eng 13:1927–1954CrossRefPubMed
45.
Okuda S, Myoui A, Ariga K, Nakase T, Yonenobu K, Yoshikawa H (2001) Mechanisms of age-related decline in insulin-like growth factor-I dependent proteoglycan synthesis in rat intervertebral disc cells. Spine 26:2421–2426CrossRefPubMed
46.
Paesold G, Nerlich AG, Boos N (2007) Biological treatment strategies for disc degeneration: potentials and shortcomings. Eur Spine J 16:447–468CrossRefPubMed
47.
Phillips FM, An H, Kang JD, Boden SD, Weinstein J (2003) Biologic treatment for intervertebral disc degeneration: summary statement. Spine 28:S99CrossRefPubMed
48.
Rajpurohit R, Risbud MV, Ducheyne P, Vresilovic EJ, Shapiro IM (2002) Phenotypic characteristics of the nucleus pulposus: expression of hypoxia inducing factor-1, glucose transporter-1 and MMP-2. Cell Tissue Res 308:401–407CrossRefPubMed
49.
Richardson SM, Hughes N, Hunt JA, Freemont AJ, Hoyland JA (2008) Human mesenchymal stem cell differentiation to NP-like cells in chitosan-glycerophosphate hydrogels. Biomaterials 29:85–93CrossRefPubMed
50.
Risbud MV, Guttapalli A, Stokes DG, Hawkins D, Danielson KG, Schaer TP, Albert TJ, Shapiro IM (2006) Nucleus pulposus cells express HIF-1 alpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem 98:152–159CrossRefPubMed
51.
Roughley P, Hoemann C, DesRosiers E, Mwale F, Antoniou J, Alini M (2006) The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation. Biomaterials 27:388–396CrossRefPubMed
52.
Slade SC, Keating JL (2007) Unloaded movement facilitation exercise compared to no exercise or alternative therapy on outcomes for people with nonspecific chronic low back pain: a systematic review. J Manip Physiol Ther 30:301–311CrossRef
53.
Sive JI, Baird P, Jeziorsk M, Watkins A, Hoyland JA, Freemont AJ (2002) Expression of chondrocyte markers by cells of normal and degenerate intervertebral discs. Mol Pathol 55:91–97CrossRefPubMed
54.
Sobajima S, Vadala G, Shimer A, Kim JS, Gilbertson LG, Kang JD (2008) Feasibility of a stem cell therapy for intervertebral disc degeneration. Spine J 8:888–896CrossRefPubMed
55.
Specchia N, Pagnotta A, Toesca A, Greco F (2002) Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine. Eur Spine J 11:145–151CrossRefPubMed
56.
Steck E, Bertram H, Abel R, Chen B, Winter A, Richter W (2005) Induction of intervertebral disc-like cells from adult mesenchymal stem cells. Stem Cells 23:403–411CrossRefPubMed
57.
Takada T, Nishida K, Doita M, Kurosaka M (2002) Fas ligand exists on intervertebral disc cells: a potential molecular mechanism for immune privilege of the disc. Spine 27:1526–1530CrossRefPubMed
58.
Tapp H, Deepe R, Ingram JA, Kuremsky M, Hanley EN Jr, Gruber HE (2008) Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix. Arthr Res Ther 10:R89CrossRef
59.
Tapp H, Hanley EN Jr, Patt JC, Gruber HE (2009) Adipose-derived stem cells: characterization and current application in orthopaedic tissue repair. Exp Biol Med (Maywood) 234:1–9CrossRef
60.
Tsai TT, Guttapalli A, Oguz E, Chen LH, Vaccaro AR, Albert TJ, Shapiro IM, Risbud MV (2007) Fibroblast growth factor-2 maintains the differentiation potential of nucleus pulposus cells in vitro: implications for cell-based transplantation therapy. Spine 32:495–502CrossRefPubMed
61.
Vadalà G, Sobajima S, Lee JY, Huard J, Denaro V, Kang JD, Gilbertson LG (2008) In vitro interaction between muscle-derived stem cells and nucleus pulposus cells. Spine J 8:804–809CrossRefPubMed
62.
Walsh AJ, Bradford DS, Lotz JC (2004) In vivo growth factor treatment of degenerated intervertebral discs. Spine 29:156–163CrossRefPubMed
63.
Wei A, Brisby H, Chung SA, Diwan AD (2008) Bone morphogenetic protein-7 protects human intervertebral disc cells in vitro from apoptosis. Spine J 8:466–474CrossRefPubMed
64.
Yamamoto Y, Mochida J, Sakai D, Nakai T, Nishimura K, Kawada H, Hotta T (2004) Upregulation of the viability of nucleus pulposus cells by bone marrow-derived stromal cells: significance of direct cell-to-cell contact in coculture system. Spine 29(14):1508–1514CrossRefPubMed
Metadaten
Titel
Nucleus pulposus tissue engineering: a brief review
verfasst von
Xinlin Yang
Xudong Li
Publikationsdatum
01.11.2009
Verlag
Springer-Verlag
Erschienen in
European Spine Journal / Ausgabe 11/2009
Print ISSN: 0940-6719
Elektronische ISSN: 1432-0932
DOI
https://doi.org/10.1007/s00586-009-1092-8

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