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01.12.2010 | Original Article

Osteoinductivity potential of rhBMP-2 associated with two carriers in different dosages

verfasst von: Priscilla Maria Fernandes Abdala, Mamie Mizusaki Iyomasa, Sandra Sato, Maria Vitória Lopes Badra Bentley, Dimitrius Leonardo Pitol, Simone Cecílio Hallak Regalo, Selma Siéssere, João Paulo Mardegan Issa

Erschienen in: Anatomical Science International | Ausgabe 4/2010

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Abstract

The objective of this study was to evaluate bone formation after application of different doses of recombinant human bone morphogenetic protein-2 (rhBMP-2) combined with monoolein or poloxamer gels, in critical bone defects of rats. Forty-five Wistar rats were divided into nine treatment groups with five animals each: I: application of 1 µg rhBMP-2 + monoolein; II: 3 µg rhBMP-2 + monoolein; III: 7 µg rhBMP-2 + monoolein; IV: 1 µg rhBMP-2 + poloxamer; V: 3 µg rhBMP-2 + poloxamer; VI: 7 µg rhBMP-2 + poloxamer; VII: monoolein only; VIII: poloxamer only; and IX: critical bone defect only. A critical-sized defect of 6 mm diameter was produced in the left parietal bone and it was filled with gels of the above mentioned treatments. After 2 weeks, the calvarial bones were removed for histological processing. Bone formation in the groups that received poloxamer gel and rhBMP-2 was not significantly different from the control group (IX). Groups receiving monoolein and rhBMP-2 (1 and 3 µg) and those that received only the carriers (VII and VIII) had less bone formation in relation to the control. The association of rhBMP-2 to both poloxamer and monoolein did not exhibit any significant differentiation in bone formation in comparison with the control group.

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Titel: Osteoinductivity potential of rhBMP-2 associated with two carriers in different dosages
verfasst von: Priscilla Maria Fernandes Abdala
Mamie Mizusaki Iyomasa
Sandra Sato
Maria Vitória Lopes Badra Bentley
Dimitrius Leonardo Pitol
Simone Cecílio Hallak Regalo
Selma Siéssere
João Paulo Mardegan Issa
Publikationsdatum: 01.12.2010
Verlag: Springer Japan
Erschienen in: Anatomical Science International / Ausgabe 4/2010
Print ISSN: 1447-6959
Elektronische ISSN: 1447-073X
DOI: https://doi.org/10.1007/s12565-010-0075-5

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