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Graefe's Archive for Clinical and Experimental Ophthalmology

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10.04.2020 | Medical Ophthalmology

Osteopontin in vitreous and idiopathic epiretinal membranes

verfasst von: Lucia Dinice, Andrea Cacciamani, Graziana Esposito, Samanta Taurone, Raffaella Carletti, Guido Ripandelli, Marco Artico, Alessandra Micera

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 7/2020

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Abstract

Purpose

To investigate osteopontin (OPN) expression in vitreous and in related idiopathic epiretinal membranes (ERMs), with respect to VEGF-A, IL8, MIP1α, IL6, and IL33, and correlate OPN expression with disease staging.

Methods

Fifteen (15) vitreous and allied ERMs were collected at the time of therapeutic vitreoretinal surgery. Additional 5 vitreous and 10 ERMs (historical collection) were used. Biochemical and molecular analysis of OPN was performed in clear vitreous, vitreal pelleted cells, and ERMs. Double-immunofluorescence analysis (OPN – GFAP and OPN – αSMA) was performed on paraffin and whole-mounted ERMs. Vitreal OPN levels were correlated to those of VEGF-A, IL8, MIP1α, IL6, and IL33.

Results

High OPN levels were observed in vitreal samples, and OPN transcripts were amplified in vitreal cells and related ERMs. OPN immunoreactivity was found in ERMs, mainly in GFAP-bearing (Muller cells) and to a less extend in αSMA-expressing (myofibroblasts) cells. OPN levels were highest at early stages of ERM formation and positively correlated to VEGF-A and MIP1α.

Conclusions

High OPN levels in vitreous, OPN transcripts in vitreal cells/ERMs, OPN immunoreactivity in activated Müller cells and contractile myofibroblasts, as well as the correlation with VEGF-A and MIP1α fulfill the potential involvement of OPN in both inflammation and tissue remodeling that takes part in vitreoretinal interface disorders. The highest OPN levels at early stages of ERM formation would prospect OPN as a potential biomarker for disease severity.

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Titel: Osteopontin in vitreous and idiopathic epiretinal membranes
verfasst von: Lucia Dinice
Andrea Cacciamani
Graziana Esposito
Samanta Taurone
Raffaella Carletti
Guido Ripandelli
Marco Artico
Alessandra Micera
Publikationsdatum: 10.04.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 7/2020
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-020-04685-w

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