Background
Methods
Patients and tissue specimens
Variables | Gender | Age | Tumor location | Lymph node metastasis | Differentiation | TNM stage | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Male | Female | ≥60 | <60 | Middle | Lower | Negative | Positive | Well | Moderate | Poor | I | II | III | |
n | 365 | 173 | 337 | 201 | 421 | 117 | 323 | 215 | 151 | 269 | 118 | 154 | 263 | 121 |
Cell and lentivirus infection of cells
RNA isolation and RT-PCR
DNA sequencing analysis
qRT-PCR assay
Western blotting
CCK-8 assay
Apoptosis assay
In vivo tumor growth assay
Statistical analysis
Results
A613T and G462Tmutations in esophageal cancer
Gene variation | n | Proportion % | Amino acid variation | Domain |
---|---|---|---|---|
375 nt: A → G | 21 | 3.90 | 88 aa: I → V | 8kD domain |
454 nt: T → C | 19 | 3.53 | 114 aa: F → S | Thumb domain |
462 nt: G → T | 12 | 2.23 | 117 aa: E → termination mutation | Thumb domain |
466 nt: G → A | 32 | 5.95 | 118 aa: G → E | Thumb domain |
613 nt: A → T | 18 | 3.35 | 167 aa: K → I | Palm domain |
648 nt: G → C | 20 | 3.72 | 179 aa: G → R | Palm domain |
660 nt: A → G | 27 | 5.02 | 183 aa: R → G | Palm domain |
665 nt: T → C | 8 | 1.49 | 184 aa: G → G | Palm domain |
676 nt: G → A | 15 | 2.79 | 188 aa: S → N | Palm domain |
737 nt: A → T | 6 | 1.12 | 208 aa: P → P | Palm domain |
740 nt: A → G | 9 | 1.67 | 209 aa: K → K | Palm domain |
832 nt: A → G | 11 | 2.04 | 240 aa: Q → R | Palm domain |
853 nt: A → G | 7 | 1.30 | 247 aa: E → G | Palm domain |
854nt: A → C, and 855nt: A → C | 12 | 2.23 | 247 aa: E → D, and 248 aa: K → Q | Palm domain |
177–234 nt: deletion | 12 | 2.23 | 22 aa: frameshift mutation; 26 aa: termination mutation | 8kD domain |
Total | 229 | 42.57 | – | – |