nach oben

Erschienen in:

18.05.2023 | Research

Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances

verfasst von: Lucía Angeles-Estrada, Elvia Pérez-Soto, Nadia Mabel Pérez-Vielma, Modesto Gómez-López, Virginia Sánchez-Monroy

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2023

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The objective of this work was to evaluate the impact of fixed orthodontic appliances on oxidative stress (OS) and genotoxicity from oral epithelial cells.

Materials and methods

Samples of oral epithelial cells were obtained from fifty-one healthy voluntary subjects who had an indication for orthodontic treatment. The samples were obtained before treatment and after 6 and 9 months of treatment. OS was evaluated by quantitating 8-hydroxy-2’deoxyguanosine (8-OHdG) and by performing relative gene expression with antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). DNA degradation and instability were evaluated by multiplex polymerase chain reaction (PCR) and fragment analysis for human identification.

Results

The quantitation results showed that 8-OHdG increased during treatment, although this increase was not statistically significant. SOD increased by 2.5- and 2.6-fold after 6 and 9 months of treatment, respectively. CAT increased by threefold after 6 months of treatment, while after 9 months of treatment, the expression level decreased to a level similar to that before treatment. DNA degradation was found in 8% and 12% of DNA samples after 6 and 9 months of treatment, respectively, while DNA instability was detected in only 2% and 8% of DNA samples after 6 and 9 months of treatment, respectively.

Conclusions

The results showed that OS and genotoxicity slightly changed after treatment with a fixed orthodontic appliance; in addition, a biological adaptation response to the treatment may occur after 6 months.

Clinical relevance

OS and genotoxicity in the buccal cavity are risk factors for oral and systemic diseases. This risk may be reduced through antioxidant supplementation, by using thermoplastic materials, or by reducing the orthodontic treatment time.

Siti Salsabila Kirana, Elza Ibrahim Auerkari (2021) Genotoxicity of NiTi orthodontic wires induced by the release of metal ions. AIP Conf Proc 2344:020008. https://doi.org/10.1063/5.0047255

Bal W, Protas AM, Kasprzak KS (2011) Genotoxicity of metal ions: chemical insights. Met Ions Life Sci 8:319–373PubMed

Sreedhara A, Cowan JA (2001) Catalytic hydrolysis of DNA by metal ions and complexes. J Biol Inorg Chem 6:337–347. https://doi.org/10.1007/s007750100209CrossRefPubMed

Katada H, Komiyama M (2009) Artificial restriction DNA cutters as new tools for gene manipulation. Chem Bio Chem 10:1279–1288. https://doi.org/10.1002/cbic.200900040CrossRefPubMed

Camargo MA, Neves A, Bortoluzzi AJ, Szpoganicz B, Martendal A, Murgu M, Fischer FL, Terenzi H, Severino PC (2008) New gadolinium complex with efficient hydrolase-like activity: a 100-million-fold rate enhancement in diester hydrolysis. Inorg Chem 47:2919–2921. https://doi.org/10.1021/ic702167pCrossRefPubMed

Beyersmann D, Hartwig A (2008) Carcinogenic metal compounds: recent insight into molecular and cellular mechanisms. Arch Toxicol 82:493–512. https://doi.org/10.1007/s00204-008-0313-yCrossRefPubMed

Hartwig A, Schwerdtle T (2002) Interactions by carcinogenic metal compounds with DNA repair processes: toxicological implications. Toxicol Lett 127:47–54. https://doi.org/10.1016/s0378-4274(01)00482-9CrossRefPubMed

Das KT, Wati MR, Fatima-Shad K (2014) Oxidative stress gated by Fenton and Haber Weiss reactions and its association with Alzheimer’s disease. Arch Neurosci 2:e60038. https://doi.org/10.5812/archneurosci.20078CrossRef

Primožič J, Poljšak B, Jamnik P, Kovač V, ČanadiJurešić G, Spalj S (2021) Risk assessment of oxidative stress induced by metal ions released from fixed orthodontic appliances during treatment and indications for supportive antioxidant therapy: a narrative review. Antioxidants (Basel) 10:1359. https://doi.org/10.3390/antiox10091359CrossRefPubMed

10.

Adwas AA, Elsayed ASI, Azab AE, Quwaydir FA (2019) Oxidative stress and antioxidant mechanisms in human body. J Appl Biotechnol Bioeng 6:43–47. https://doi.org/10.15406/jabb.2019.06.00173

11.

Bayir H (2005) Reactive oxygen species. Crit Care Med 33:S498-501. https://doi.org/10.1097/01.ccm.0000186787.64500.12CrossRefPubMed

12.

Chiorcea-Paquim AM (2022) 8-oxoguanine and 8-oxodeoxyguanosine biomarkers of oxidative DNA damage: a review on HPLC-ECD determination. Molecules 27:1620. https://doi.org/10.3390/molecules27051620CrossRefPubMedPubMedCentral

13.

Kalyanaraman B (2013) Teaching the basics of redox biology to medical and graduate students: oxidants, antioxidants and disease mechanisms. Redox Biol 1:244–257. https://doi.org/10.1016/j.redox.2013.01.014CrossRefPubMedPubMedCentral

14.

Morii A, Miyamura Y, Sago MI, Mizuhara M, Shikayama T, Naniwa M, Hitomi S, Ujihara I, Kuroishi KN, Gunjigake KK, Shiga M, Morimoto Y, Kawamoto T, Ono K (2020) Orthodontic force-induced oxidative stress in the periodontal tissue and dental pulp elicits nociception via activation/sensitization of TRPA1 on nociceptive fibers. Biol Med 147:175–186. https://doi.org/10.1016/j.freeradbiomed.2019.12.016CrossRef

15.

Ozel N, Aksoy A, Kırzıoglu FY, Doguc DK, Aksoy TA (2018) Evaluation of interleukin-1β level and oxidative status in gingival crevicular. Arch Oral Biol 90:74–79. https://doi.org/10.1016/j.archoralbio.2018.02.020CrossRefPubMed

16.

Díaz-Rojas AR, Angeles-Estrada L, Pérez-Vielma NM, Sánchez-Monroy V (2022) Short tandem repeat (STR) instability in the oral mucosa of patients submitted to fixed orthodontic therapy: a limitation of STR profile quality for human identification. Forensic Sci Med Pathol 18:57–63. https://doi.org/10.1007/s12024-021-00451-4CrossRefPubMed

17.

Buljan ZI, Ribaric SP, Abram M, Ivankovic A, Spalj S (2012) In vitro oxidative stress induced by conventional and self-ligating brackets. Angle Orthod 82:340–345. https://doi.org/10.2319/061811-395.1CrossRefPubMed

18.

Nimeri G, Curry J, Berzins D, Liu D, Ahuja B, Lobner D (2021) Cytotoxic evaluation of two orthodontic silver solder materials on human periodontal ligament fibroblast cells and the effects of antioxidant and antiapoptotic reagents. Angle Orthod 91:349–355. https://doi.org/10.2319/050620-405.1CrossRefPubMedPubMedCentral

19.

Gonçalves TS, Menezes LM, Trindade C, Machado Mda S, Thomas P, Fenech M, Henriques JA (2014) Citotoxic and genotoxicity of orthodontic bands with or without silver soldered joints. Mut Res Genet Toxicol Environ Mutagen 762:1–8. https://doi.org/10.1016/j.mrgentox.2014.01.011CrossRef

20.

Nogueira LS, Vasconcelos CP, Mitre GP, da Silva Kataoka MS, Lima MO, de Oliveira EHC, Lima RR (2019) Oxidative damage in human periodontal ligament fibroblast (hPLF) after methylmercury exposure. Oxid Med Cell Longev 2019:8470857. https://doi.org/10.1155/2019/8470857CrossRefPubMedPubMedCentral

21.

Kovač V, Poljšak B, Primožič J, Jamnik P (2020) Are metal ions that make up orthodontic alloys cytotoxic, and do they induce oxidative stress in a yeast cell model? Int J Mol Sci 21:7993. https://doi.org/10.3390/ijms21217993CrossRefPubMedPubMedCentral

22.

Kovač V, Bergant M, Ščančar J, Primožič J, Jamnik P, Poljšak B (2021) Causation of oxidative stress and defense response of a yeast cell model after treatment with orthodontic alloys consisting of metal ions. Antioxidants (Basel) 11(1):63. https://doi.org/10.3390/antiox11010063CrossRefPubMed

23.

Vicente A, Bravo-González LA, Navarro JA, Buendía AJ, Camacho-Alonso F (2021) Effects of diabetes on oxidative stress, periodontal ligament fiber orientation, and matrix metalloproteinase 8 and 9 expressions during orthodontic tooth movement. Clin Oral Investig 25:1383–1394. https://doi.org/10.1007/s00784-020-03446-7CrossRefPubMed

24.

Atuğ Özcan SS, Ceylan I, Ozcan E, Kurt N, Dağsuyu IM, Canakçi CF (2014) Evaluation of oxidative stress biomarkers in patients with fixed orthodontic appliances. Dis Markers 2014:597892. https://doi.org/10.1155/2014/597892CrossRefPubMedPubMedCentral

25.

Dallel I, Ben Salem I, Merghni A, Bellalah W, Neffati F, Tobji S, Mastouri M, Ben Amor A (2020) Influence of orthodontic appliance type on salivary parameters during treatment. Angle Orthod 90:532–538. https://doi.org/10.2319/082919-562.1CrossRefPubMedPubMedCentral

26.

Orozco-Páez J, Méndez-Rodríguez MA, Rodríguez-Cavallo E, Díaz-Caballero A, Méndez-Cuadro D (2021) Protein carbonylation associated with nickel liberation in orthodontic gingival overgrowth. Arch Oral Biol 125:105103. https://doi.org/10.1016/j.archoralbio.2021.105103CrossRefPubMed

27.

Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25:402–408. https://doi.org/10.1006/meth.2001.1262CrossRefPubMed

28.

Löe H (1967) The gingival index, the plaque index and the retention index systems. J Periodontol 38:S610-616. https://doi.org/10.1902/jop.1967.38.6.610CrossRef

29.

Lombardo G, Vena F, Negri P, Barilotti, Paglia L, Colombo S, Orso M, Cianetti S (2020) Worldwide prevalence of malocclusion in the different stages of dentition: a systematic review and meta-analysis. Eur J Paediatr Dent 21:115–122. https://doi.org/10.23804/ejpd.2020.21.02.05

30.

Bellamine M, Ousehal L, Kissa J (2012) Orthodontic treatment and gingival hyperplasia: a case report. Odontostomatol Trop 35:31–41PubMed

31.

Reema FHA, Baydaa HA (2022) The impact of fixed orthodontic appliance on oxidative status and gingival health condition. J Res Med Dent Sci 10:182–187

32.

Guler C, Toy E, Ozturk F, Gunes D, Karabulut AB, Otlu O (2015) Evaluation of salivary total oxidant-antioxidant status and DNA damage of children undergoing fixed orthodontic therapy. Angle Orthod 85:239–244. https://doi.org/10.2319/110113-798.1CrossRefPubMed

33.

Buczko P, Knaś M, Grycz M, Szarmach I, Zalewska A (2017) Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. Adv Med Sci 62:129–135. https://doi.org/10.1016/j.advms.2016.11.004CrossRefPubMed

34.

Esenlik E, Bolat Gümüş E, EroğluAlbayrak G, KumbulDoğuç D (2021) Does puberty affect oxidative stress levels and antioxidant activity of saliva in patients with fixed orthodontic appliances? J Orofac Orthop. https://doi.org/10.1007/s00056-021-00346-7CrossRefPubMed

35.

Bender K, Farfán MJ, Schneider PM (2004) Preparation of degraded human DNA under controlled conditions. Forensic Sci Int 139:135–140. https://doi.org/10.1016/j.forsciint.2003.10.003CrossRefPubMed

36.

Brinkmann B, Klintschar M, Neuhuber F, Hühne J, Rolf B (1998) Mutation rate in human microsatellites: influence of the structure and length of the tandem repeat. Am J Hum Genet 62:1408–1415. https://doi.org/10.1086/301869CrossRefPubMedPubMedCentral

37.

Imani MM, Mozaffari HR, Ramezani M, Sadeghi M (2019) Effect of fixed orthodontic treatment on salivary nickel and chromium levels: a systematic review and meta-analysis of observational studies. Dent J (Basel) 7:21. https://doi.org/10.3390/dj7010021CrossRefPubMed

38.

Heravi F, Abbaszadegan MR, Merati M, Hasanzadeh N, Dadkhah E, Ahrari F (2013) DNA damage in oral mucosa cells of patients with fixed orthodontic appliances. J Dent 10:494–500

39.

Flores-Bracho MG, Takahashi C, Castillo W, Saraiva M, Küchler E, Matsumoto MAN, Ferreira JTL, Nelson-Filho P, Romano FL (2019) Genotoxic effects in oral mucosal cells caused by the use of orthodontic fixed appliances in patients after short and long periods of treatment. Clin Oral Invest 23:2913–2919. https://doi.org/10.1007/s00784-018-02795-8CrossRef

40.

Mariachelliah SJL, Sathiasekar AC, Mathew DG, Ramachandran KL, Kesavaram P, Kangasabapathy UG (2019) Localized toxicity in patients with fixed orthodontic appliance: a case control study. Indian J Public Health Res Dev 10:989–994. https://doi.org/10.37506/v10/i12/2019/ijphrd/192253

Titel: Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances
verfasst von: Lucía Angeles-Estrada
Elvia Pérez-Soto
Nadia Mabel Pérez-Vielma
Modesto Gómez-López
Virginia Sánchez-Monroy
Publikationsdatum: 18.05.2023
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2023
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-023-05039-6

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Oxidative stress and genotoxicity in oral epithelial cells from subjects undergoing orthodontic treatment with fixed appliances