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Neurotoxicity Research

Erschienen in:

01.05.2015 | Short Report/Rapid Communication

PDGF-BB Protects Mitochondria from Rotenone in T98G Cells

verfasst von: Ricardo Cabezas, Marcos Fidel Avila, Janneth González, Ramon Santos El-Bachá, George E. Barreto

Erschienen in: Neurotoxicity Research | Ausgabe 4/2015

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Abstract

Rotenone is one of the most-studied neurotoxic substances as it induces oxidative stress processes both in cellular and animal models. Rotenone affects ATP generation, reactive oxygen species (ROS) production, and mitochondrial membrane potential in neurons and astrocyte-like cells. Previous epidemiologic studies have supported the role of neurotrophic factors such as BDNF and GDNF in neuroprotection mainly in neurons; however, only very few studies have focused on the importance of astrocytic protection in neurodegenerative models. In the present study, we assessed the neuroprotective effects of PDGF-BB against toxicity induced by rotenone in the astrocytic-like model of T98G human glioblastoma cell line. Our results demonstrated that pretreatment with PDGF-BB for 24 h increased cell viability, preserved nuclear morphology and mitochondrial membrane potential following stimulation with rotenone, and reduced ROS production nearly to control conditions. These observations were accompanied by important morphological changes induced by rotenone and that PDGF-BB was able to preserve cellular morphology under this toxic stimuli. These findings indicated that PDGF-BB protects mitochondrial functions, and may serve as a potential therapeutic strategy in rotenone-induced oxidative damage in astrocytes.

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Titel: PDGF-BB Protects Mitochondria from Rotenone in T98G Cells
verfasst von: Ricardo Cabezas
Marcos Fidel Avila
Janneth González
Ramon Santos El-Bachá
George E. Barreto
Publikationsdatum: 01.05.2015
Verlag: Springer US
Erschienen in: Neurotoxicity Research / Ausgabe 4/2015
Print ISSN: 1029-8428
Elektronische ISSN: 1476-3524
DOI: https://doi.org/10.1007/s12640-014-9509-5

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