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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.09.2010 | Original Paper

Peripheral blood gene expression of B7 and CD28 family members associated with tumor progression and microscopic lymphovascular invasion in colon cancer patients

verfasst von: Hyunji Lee, Ji Hyun Kim, Sung Yeun Yang, Jihye Kong, Minkyung Oh, Dae Hoon Jeong, Jae-il Chung, Ki Beom Bae, Jin Yong Shin, Kwan Hee Hong, Inhak Choi

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 9/2010

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Abstract

Purpose

To associate the global gene expression of B7/CD28 family transcripts with pathologic features of colon cancer, we determined the B7/CD28 family transcripts in peripheral blood mononuclear cells (PBMCs) from normal subjects and patients with adenomatous polyps and colon cancer, and correlated the results with pathologic features of colon cancer.

Methods

PBMCs from age-matched normal subjects and patients with adenomatous polyps and colon cancer were analyzed for peripheral blood transcripts (PBTs) of B7/CD28 family using real-time PCR. Differences in expression levels of B7/CD28 PBTs across all cancer stages and between colon cancer patients with or without microscopic lymphovascular invasion (LVI) were analyzed.

Results

The results showed a significant upregulation of PBTs of co-inhibitory molecules such as B7-H3 and PD-1 and a significant PBT downregulation of co-stimulatory molecules including CD28 and ICOS in colon cancer patients. Furthermore, the increase of B7-H3 PBT was strongly associated with tumor invasion (P = 0.025) and advanced TNM stages (P = 0.019), whereas the decline of co-stimulatory ligand B7-H2 PBT was related to regional lymph node metastasis (P = 0.028) and aggressive tumor invasion (P = 0.031). In addition, the ratios of PBT expression of CD28 family to B7 family such as CTLA-4 to B7-H2 and PD-1 to B7-H2 were significantly higher in colon cancer patients with microscopic LVI than in those without LVI (P = 0.001 and P = 0.016, respectively).

Conclusions

Our results suggest that B7/CD28 family PBTs may serve as valuable markers reflecting the pathological features of colon cancer.

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Titel: Peripheral blood gene expression of B7 and CD28 family members associated with tumor progression and microscopic lymphovascular invasion in colon cancer patients
verfasst von: Hyunji Lee
Ji Hyun Kim
Sung Yeun Yang
Jihye Kong
Minkyung Oh
Dae Hoon Jeong
Jae-il Chung
Ki Beom Bae
Jin Yong Shin
Kwan Hee Hong
Inhak Choi
Publikationsdatum: 01.09.2010
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 9/2010
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-010-0800-4

Springer Medizin

Peripheral blood gene expression of B7 and CD28 family members associated with tumor progression and microscopic lymphovascular invasion in colon cancer patients