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Lasers in Medical Science

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23.07.2019 | Original Article

Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells

verfasst von: Fatemeh Zare, Mohammad Bayat, Abbas Aliaghaei, Abbas Piryaei

Erschienen in: Lasers in Medical Science | Ausgabe 3/2020

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Abstract

Pathophysiologic conditions associated with diabetes mellitus affect mesenchymal stem cells (MSCs), and this phenomenon may lead to some diabetic secondary complications. The present study was conducted to evaluate the impact of photobiomodulation (PBM) on rat diabetic MSC (DMSC) behavior in vitro. For the purpose of PBM, we used helium-neon laser with a wavelength of 632.8 nm at three different energy densities (0.5, 1, 2 J/cm²) and radiation periodicity of once, twice, and thrice. The survival, proliferation, and apoptosis in the normal MSCs (NMSCs), DMSCs, and diabetic MSCs, which were laser irradiated (DMSCs+L), were assessed using MTT assay, Ki67 immunofluorescence staining, and TUNEL assay, respectively. Our results demonstrated that DMSCs have significantly lower survival (P < 0.05) and proliferation rates (P < 0.001), and dramatically higher population doubling time (PDT, P < 0.001) and apoptosis rates (P < 0.001) as compared to NMSCs. Moreover, PBM with energy density of 1 J/cm² and the periodicity of 1 or 2 times could improve diabetic MSC capabilities in the term of survival, proliferation, and apoptosis. Considering these findings, it is suggested that PBM could improve the ability of diabetic MSCs in vitro prior to transplantation or may rise their capabilities in their native niche in vivo.

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Titel: Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells
verfasst von: Fatemeh Zare
Mohammad Bayat
Abbas Aliaghaei
Abbas Piryaei
Publikationsdatum: 23.07.2019
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 3/2020
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02844-y

Springer Medizin

Abstract

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