Background
Methods
Collection and identification of plant material
Preparation of plant material and fractionation
Screening of phytochemical constituents
Alkaloid detection
Anthocyanin and betacyanin detection
Anthraquinone detection
Coumarins detection
Flavonoids detection
Glycosides detection
Saponin detection
Tannin detection
Terpenoid detection
Phenol assessment
Sterol detection
Steroid and Phytosteroid detection
Phytosterol detection
Phlobatannins detection
Triterpenoids detection
Quinones detection
Acid detection
Vitamin C detection
Protein detection
Carbohydrate detection
Oils and resins detection
Quantitative phytochemical constituent determination
Alkaloid quantification
Phenol quantification
Flavonoid quantification
Tannin quantification
Saponin quantification
Antibacterial screening
Bacterial MIC and MBC determination
Antifungal screening
Fungal MIC and MFC determination
Statistical analysis
Results
Plant yield and fractionation
Plant sample | Percentage yield (%) |
---|---|
PJM | 50a |
PJH | 30 |
PJC | 2 |
PJE | 36 |
PJB | 12 |
PJA | 18 |
Phytochemical qualitative study
Phytochemical | PJM | PJH | PJC | PJE | PJB | PJA |
---|---|---|---|---|---|---|
Terpenoids | + | + | + | – | – | – |
Triterpenoids | + | + | + | – | – | – |
Coumarins | + | – | + | + | + | + |
Flavonoids | ||||||
Alkaline reagent test | + | – | + | – | + | + |
FeCl3 test | + | – | + | – | + | + |
Tannins | ||||||
Alkaline reagent test | + | – | + | + | + | + |
FeCl3 test | + | – | + | + | + | + |
Condensed Tannins | + | – | – | + | + | + |
Hydrolysable tannins | – | + | + | – | – | – |
Phlobatannins | + | – | – | + | + | – |
Steroids | + | – | – | + | + | + |
Phytosteroids | – | – | – | + | + | + |
Anthraquinones | – | – | – | – | – | – |
Phenols | + | – | + | – | – | – |
Alkaloids | ||||||
Mayer’s test | + | + | + | + | + | – |
Hager’s test | + | + | + | + | + | – |
Glycosides | + | + | + | + | + | + |
Saponins | + | – | – | + | + | – |
Sterols | + | – | – | + | – | + |
Quinones | + | + | + | + | + | + |
Acids | + | + | + | + | + | + |
Vitamin C | + | + | + | + | + | + |
Proteins | ||||||
Xanthoproteic test | + | + | + | + | + | + |
Biuret test | + | + | + | + | + | + |
Carbohydrates | – | – | – | + | + | – |
Oils and Resins | + | + | + | + | + | + |
Anthocyanin | – | – | – | – | – | – |
Betacyanin | + | – | + | – | + | + |
Phytochemical quantitative study
Percentage (%) yield per gram | mg of GAE/ g of extract | ||||
---|---|---|---|---|---|
Plant extracts | Alkaloids | Flavonoids | Saponins | Phenols | Tannins |
PJM | 12.5 ± 0.17 | 7.4 ± 0.14 | 4.4 ± 0.42 | 611.5 ± 2.18 | 159.4 ± 1.85 |
PJH | 0.71 ± 0.21 | NT | NT | NT | NT |
PJC | 7.7 ± 0.14 | 3.5 ± 0.07 | NT | 342.8 ± 1.69 | 203.8 ± 2.67 |
PJE | 22.5 ± 0.5 | NT | 5.5 ± 0.28 | NT | 244.3 ± 2.03 |
PJB | 5.7 ± 0.36 | 6.6 ± 0.42 | 1.8 ± 0.11 | NT | 231.6 ± 1.93 |
PJA | NT | 4.6 ± 0.11 | NT | NT | 110.5 ± 0.74 |
Antibacterial activity of P. jacquemontiana
Extract/Fractions (1000 μg/disc) | ||||||
---|---|---|---|---|---|---|
Isolate (s) | PJM | PJH | PJC | PJE | PJB | PJA |
Staphylococcus aureus | ||||||
MDR (6301) | 24.16 ± 1.25 | 27.66 ± 0.28 | 25.16 ± 1.04 | 28 ± 1.32 | 24.16 ± 1.04 | – |
MDR (3884) | 31.16 ± 2.02 | 29 ± 1.0 | 24.83 ± 0.28 | 26.5 ± 0.5 | 28.66 ± 0.28 | – |
MRSA (12861) | 22.5 ± 1.32 | 23.16 ± 1.25 | 25 ± 0.5 | 26 ± 1.0 | 25.33 ± 1.04 | – |
MDR (9861) | 25.16 ± 0.04 | 22.5 ± 0.5 | 26.33 ± 1.25 | 26.5 ± 0.86 | 28.83 ± 0.76 | – |
(5764) | 29 ± 1.0 | 26 ± 1.0 | 27.33 ± 0.57 | 27 ± 1,0 | 23.5 ± 0.5 | – |
(31414) | 26 ± 1.0 | 32 ± 0.86 | 30.33 ± 1.44 | 22.5 ± 0.5 | 25 ± 1.0 | – |
Staphylococcus lugdenesis | ||||||
MDR (6197) | 35.5 ± 1.32 | 28.5 ± 0.5 | 24.83 ± 1.04 | 26.5 ± 0.5 | 26.83 ± 0.28 | – |
(4338) | 24 ± 1.0 | 18 ± 1.0 | - | 25.66 ± .57 | 20.5 ± 0.86 | – |
Escherichia coli | ||||||
MDR (52331) | 27.83 ± 0.76 | 28.83 ± 0.28 | 24.83 ± 1.25 | 24.33 ± 0.57 | 31.16 ± 0.76 | – |
(22244) | 28 ± 0.5 | 13.16 ± 0.28 | 14 ± 1.0 | 27.83 ± 0.76 | 23.5 ± 0.86 | – |
“a” | 23.5 ± 0.5 | - | - | 24.16 ± 0.76 | 18.33 ± 1.04 | – |
“b” | 24.5 ± 0.5 | - | - | 25.66 ± 0.28 | 19 ± 1.0 | – |
Klebsiella pneumoniae | ||||||
MDR (82431) | 30 ± 1.0 | 26 ± 2.0 | 26.66 ± 0.28 | 25.16 ± 0.28 | 33.33 ± 0.57 | – |
ESBL MDR (87005) | 35.33 ± 0.57 | 34 ± 1.0 | 29.5 ± 0.5 | 26 ± 1.0 | 28.16 ± 0.76 | – |
(34529) | 27.83 ± 0.28 | 17.16 ± 1.04 | 18 ± 1.0 | 21.83 ± 1.04 | 20 ± 1.0 | – |
(35967) | 22.16 ± 0.76 | - | - | 20.83 ± 1.04 | 19 ± 0.86 | – |
“a” | 26 ± 2.0 | - | - | 23.83 ± 0.57 | 19 ± 0.5 | – |
Pseudomonas aeruginosa | ||||||
(27853) | 25.16 ± 0.76 | 21.5 ± 0.5 | 14 ± 2.0 | 19.66 ± 1.52 | 18.33 ± 0.57 | – |
Coagulase negative staphylococci | ||||||
(12731) | 28 ± 2.0 | 27.33 ± 0.57 | 22 ± 1.0 | 25.16 ± 1.04 | 26.16 ± 0.76 | – |
MIC and MBC of P. jacquemontiana
Extract/Fractions (μg /ml) | |||||||||
---|---|---|---|---|---|---|---|---|---|
PJM | PJH | PJC | |||||||
Isolate(s) | MIC | MBC | MBC/MIC | MIC | MBC | MBC/MIC | MIC | MBC | MBC/MIC |
Staphylococcus aureus | |||||||||
MDR (6301) | 32 | 64 | 02 | 64 | 256 | 04 | 128 | 512 | 04 |
MDR (3884) | 16 | 64 | 04 | 128 | 256 | 02 | 128 | 256 | 02 |
MRSA (12861) | 08 | 32 | 04 | 16 | 128 | 08 | 32 | 128 | 04 |
MDR (9861) | 32 | 64 | 02 | 32 | 128 | 04 | 128 | 256 | 02 |
(5764) | 16 | 128 | 08 | 128 | 256 | 02 | 64 | 256 | 04 |
(31414) | 08 | 32 | 04 | 16 | 128 | 08 | 32 | 256 | 08 |
Staphylococcus lugdenesis | |||||||||
MDR (6197) | 32 | 128 | 04 | 64 | 128 | 02 | 512 | 512 | 01 |
(4338) | 16 | 64 | 04 | 64 | 256 | 04 | – | – | – |
Escherichia coli | |||||||||
MDR (52331) | 32 | 128 | 04 | 64 | 128 | 02 | 256 | 512 | 02 |
(22244) | 08 | 64 | 08 | 32 | 64 | 02 | 64 | 256 | 04 |
“a” | 08 | 32 | 04 | – | – | – | – | – | – |
“b” | 08 | 32 | 04 | – | – | – | – | – | – |
Klebsiella pneumoniae | |||||||||
MDR (82431) | 64 | 256 | 04 | 128 | 512 | 04 | 128 | 512 | 04 |
ESBL MDR (87005) | 16 | 64 | 04 | 64 | 256 | 04 | 256 | 512 | 02 |
(34529) | 08 | 32 | 04 | 08 | 64 | 08 | 32 | 128 | 04 |
(35967) | 08 | 32 | 04 | – | – | – | – | – | – |
“a” | 08 | 64 | 08 | – | – | – | – | – | – |
Pseudomonas aeruginosa | |||||||||
(27853) | 08 | 64 | 08 | 32 | 128 | 04 | 128 | 256 | 02 |
Coagulase negative staphylococci | |||||||||
(12731) | 64 | 128 | 02 | 64 | 256 | 04 | 128 | 512 | 04 |
Extract/Fractions (μg/ml) | Antibiotic used (μg/ml) | ||||||||
---|---|---|---|---|---|---|---|---|---|
PJE | PJB | Cefixime | |||||||
Clinical Isolate(s) | MIC | MBC | MBC/MIC | MIC | MBC | MBC/MIC | MIC | MBC | MBC/MIC |
Staphylococcus aureus | |||||||||
MDR (6301) | 32 | 64 | 02 | 128 | 256 | 02 | 16 | 64 | 04 |
MDR (3884) | 08 | 32 | 04 | 64 | 256 | 04 | 08 | 16 | 02 |
MRSA (12861) | 08 | 64 | 08 | 32 | 128 | 04 | 64 | 64 | 01 |
MDR (9861) | 32 | 128 | 04 | 64 | 128 | 02 | 64 | 128 | 02 |
(5764) | 32 | 128 | 04 | 64 | 256 | 04 | 32 | 64 | 02 |
(31414) | 16 | 64 | 04 | 08 | 64 | 08 | 08 | 16 | 02 |
Staphylococcus lugdenesis | |||||||||
MDR (6197) | 32 | 128 | 04 | 512 | 512 | 01 | 32 | 32 | 01 |
(4338) | 16 | 64 | 04 | 32 | 128 | 04 | 08 | 32 | 04 |
Escherichia coli | |||||||||
MDR (52331) | 64 | 128 | 02 | 128 | 128 | 01 | 128 | 128 | 01 |
(22244) | 08 | 32 | 04 | 16 | 64 | 04 | 16 | 32 | 02 |
“a” | 08 | 64 | 08 | 32 | 128 | 04 | 16 | 32 | 02 |
“b” | 08 | 64 | 08 | 32 | 128 | 04 | 08 | 32 | 04 |
Klebsiella pneumoniae | |||||||||
MDR (82431) | 32 | 256 | 08 | 32 | 128 | 04 | 128 | 128 | 01 |
ESBL MDR (87005) | 16 | 64 | 04 | 32 | 256 | 08 | 64 | 128 | 02 |
(34529) | 08 | 32 | 04 | 08 | 64 | 08 | 08 | 16 | 02 |
(35967) | 08 | 64 | 08 | 16 | 128 | 08 | 64 | 128 | 02 |
“a” | 08 | 64 | 08 | 16 | 64 | 04 | 02 | 08 | 04 |
Pseudomonas aeruginosa | |||||||||
(27853) | 16 | 64 | 04 | 08 | 128 | 16 | 08 | 64 | 08 |
Coagulase negative staphylococci | |||||||||
(12731) | 32 | 128 | 04 | 32 | 128 | 04 | 64 | 128 | 02 |
Antifungal activity of P. jacquemontiana
Extract/fractions (1000 μg/disc) | ||||||
---|---|---|---|---|---|---|
Fungal isolates | PJM | PJH | PJC | PJE | PJB | PJA |
F. solani ATCC 36031 | 20.33 ± 1.25 | – | – | 24.83 ± 1.04 | 17.33 ± 1.52 | – |
A. niger ATCC 6275 | 22.66 ± 2.08 | – | – | 21.33 ± 1.52 | 23.66 ± .57 | – |
A. flavus ATCC 204304 | 29 ± 1.0 | – | – | 23.33 ± 1.25 | 15.16 ± 1.04 | – |
M. piriformis ATCC 52554 | 26.66 ± 0.57 | – | – | 18.66 ± 0.28 | 24 ± 2.0 | – |
C. albicans (90028) | 23.33 ± 1.25 | – | – | 25.5 ± 0.5 | 25.83 ± 0.28 | – |
D. hansenii
| 25 ± 1.0 | – | – | 26.83 ± 0.76 | 21.66 ± 0.28 | – |
W. anomalus (KU949596) | 27.33 ± 0.76 | – | – | 25.16 ± 1.60 | 22.5 ± 1.32 | – |
W. anomalus (KU949595) | 25.33 ± 1.52 | – | – | 28 ± 1.0 | 19.83 ± 1.25 | – |
MIC and MFC of P. jacquemontiana
Extract/Fractions (μg/ml) | Standard (μg/ml) | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
PJM | PJE | PJB | Clotrimazole | |||||||||
Clinical Isolate(s) | MIC | MFC | MFC/MIC | MIC | MFC | MFC/MIC | MIC | MFC | MFC/MIC | MIC | MFC | MFC/MIC |
F. solani ATCC 36031 | 32 | 128 | 04 | 32 | 64 | 02 | 32 | 128 | 04 | 04 | 08 | 02 |
A. niger ATCC 6275 | 16 | 32 | 02 | 16 | 128 | 08 | 128 | 256 | 02 | 32 | 64 | 02 |
A. flavus ATCC 204304 | 08 | 32 | 04 | 32 | 256 | 08 | 128 | 512 | 04 | 32 | 128 | 04 |
M. piriformis ATCC 52554 | 32 | 128 | 04 | 08 | 128 | 16 | 32 | 64 | 02 | 08 | 16 | 02 |
C. albicans (90028) | 64 | 256 | 04 | 64 | 256 | 04 | 64 | 128 | 02 | 16 | 64 | 04 |
D. hansenii
| 64 | 256 | 04 | 64 | 512 | 08 | 32 | 512 | 16 | 64 | 128 | 02 |
W. anomalus (KU949596) | 08 | 64 | 08 | 32 | 64 | 02 | 32 | 256 | 08 | 08 | 32 | 04 |
W. anomalus (KU949595) | 16 | 32 | 02 | 08 | 32 | 04 | 64 | 512 | 08 | 16 | 64 | 04 |