Background
Methods
Ethics statement
Participants
Sample collection
Metabolomic analysis
Sample pretreatment
Gas chromatography–mass spectrometry (GC-MS) analysis
Chromatographic condition
Quadrupole–time-of-flight conditions
Data analysis
Results
Clinical characteristics
Inter-group PCA analysis
Inter-group PLS-DA analysis
Inter-group volcano plot analysis
Metabolites with significant differences between groups
Adduct | Description | Fold change |
P-value | VIP | m/z | rt s |
---|---|---|---|---|---|---|
(M + H)+ | N-Acetyl-L-alanine | 1.44 | 0.031 | 1.62 | 132.0651 | 62.14 |
(M + H)+ | N1-Methyl-2-pyridone-5-carboxamide | 0.67 | 0.033 | 1.73 | 153.0652 | 207.81 |
(M + H)+ | L-Tyrosine | 1.14 | 0.031 | 1.09 | 182.0812 | 66.74 |
(M + H)+ | L-Phenylalanine | 1.25 | 0.014 | 1.58 | 166.0857 | 241.44 |
(M + H)+ | L-Palmitoylcarnitine | 0.79 | 0.025 | 1.39 | 400.3424 | 619.41 |
(M + H)+ | L-Methionine | 1.12 | 0.038 | 1.08 | 150.0578 | 66.42 |
(M + H)+ | L-Arginine | 1.22 | 0.094 | 1.09 | 175.1186 | 49.01 |
(M + H-H2O)+ | Isomaltose | 1.22 | 0.010 | 1.34 | 325.1125 | 50.74 |
(M + H)+ | Hydrocortisone | 0.76 | 0.060 | 1.01 | 363.2165 | 426.53 |
(M + H)+ | Biliverdin | 0.71 | 0.011 | 1.43 | 583.2533 | 488.13 |
KEGG metabolic pathway analysis of different metabolites
ID | Metabolic pathway name | Cpd |
---|---|---|
map01230 | Biosynthesis of amino acids (7) | C00062,C00073,C00078,C00079,C00082,C00148,C00624 |
map00970 | Aminoacyl-tRNA biosynthesis (6) | C00062,C00073,C00078,C00079,C00082,C00148 |
map01130 | Biosynthesis of antibiotics (6) | C00062,C00078,C00079,C00082,C00148,C00624 |
map05230 | Central carbon metabolism in cancer (6) | C00062,C00073,C00078,C00079,C00082,C00148 |
map04974 | Protein digestion and absorption (6) | C00062,C00073,C00078,C00079,C00082,C00148 |
map01060 | Biosynthesis of plant secondary metabolites (6) | C00062,C00073,C00078,C00079,C00082,C00408 |
map01210 | 2-Oxocarboxylic acid metabolism (5) | C00073,C00078,C00079,C00082,C00624 |
map00966 | Glucosinolate biosynthesis (4) | C00073,C00078,C00079,C00082 |
map04978 | Mineral absorption (4) | C00073,C00078,C00079,C00148 |
map02010 | ABC transporters (4) | C00062,C00079,C00148,C01487 |
map01063 | Biosynthesis of alkaloids derived from shikimate pathway (3) | C00078,C00079,C00082 |
map01070 | Biosynthesis of plant hormones (3) | C00073,C00078,C00079 |
map01064 | Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid (3) | C00062,C00079,C00408 |
map00400 | Phenylalanine, tyrosine and tryptophan biosynthesis (3) | C00078,C00079,C00082 |