Erschienen in:
01.12.1999 | Paper Report
Post-transcriptional regulation of TNF gene expression in vivo
verfasst von:
Andy Clark
Erschienen in:
Arthritis Research & Therapy
|
Ausgabe 1/2000
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Excerpt
The 3'-untranslated region (UTR) of tumor necrosis factor (TNF) mRNA contains an adenosine-uracil-rich element (ARE), with seven repeats of the pentamer sequence AUUUA. Such sequences are known to participate in the regulation of mRNA stability and translation. On the basis of transfection experiments using reporter gene constructs and immortalised myeloid cell lines, the TNF 3'-UTR appears to participate in post-transcriptional regulation of TNF gene expression. However, the role of the TNF 3'-UTR in the regulation of gene expression has not been directly demonstrated in vivo. To generate a murine cell line bearing a precise deletion of the 69 bp ARE from the TNF 3'-UTR. To examine the physiology of this 'knockout' line, and any effects upon TNF gene expression. …