Background
Materials and methods
Sample selection and preparation
Prediction of antigenicity
Tertiary structure prediction
Results
Characterization of HBV mutants
Prediction of antigenicity
Position | M54923 | Pattern T123A* | Pattern T123N** | Pattern M133L* | Pattern M133I** | Pattern M133T** | Pattern M133V** | Pattern T143M* | Pattern T143L** | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | Residue | Antigenic Index | |
110 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 | Ile | -0.2 |
111 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 |
112 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 | Gly | 0.35 |
113 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 | Ser | 0.8 |
114 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 | Ser | 1 |
115 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 | Thr | 0.4 |
116 | Thr | 1.05 | Thr | 1.05 | Thr |
0.8
| Thr | 1.05 | Thr | 1.05 | Thr | 1.05 | Thr | 1.05 | Thr | 1.05 | Thr | 1.05 |
117 | Ser | 1.3 | Ser | 1.3 | Ser |
1.05
| Ser | 1.3 | Ser | 1.3 | Ser | 1.3 | Ser | 1.3 | Ser | 1.3 | Ser | 1.3 |
118 | Thr | 1.2 | Thr | 1.2 | Thr |
0.95
| Thr | 1.2 | Thr | 1.2 | Thr | 1.2 | Thr | 1.2 | Thr | 1.2 | Thr | 1.2 |
119 | Gly | 2.05 | Gly | 2.05 | Gly |
1.6
| Gly | 2.05 | Gly | 2.05 | Gly | 2.05 | Gly | 2.05 | Gly | 2.05 | Gly | 2.05 |
120 | Pro | 2.5 | Pro | 2.5 | Pro |
2.05
| Pro | 2.5 | Pro | 2.5 | Pro | 2.5 | Pro | 2.5 | Pro | 2.5 | Pro | 2.5 |
121 | Cys | 2.25 | Cys | 2.25 | Cys |
2.5
| Cys | 2.25 | Cys | 2.25 | Cys | 2.25 | Cys | 2.25 | Cys | 2.25 | Cys | 2.25 |
122 | Lys | 2 | Lys |
1.6
| Lys |
2.25
| Lys | 2 | Lys | 2 | Lys | 2 | Lys | 2 | Lys | 2 | Lys | 2 |
123
| Thr | 0.35 |
Ala
|
0.4
|
Asn
|
2
| Thr | 0.35 | Thr | 0.35 | Thr | 0.35 | Thr | 0.35 | Thr | 0.35 | Thr | 0.35 |
124 | Cys | 0.25 | Cys |
0.15
| Cys |
1.5
| Cys | 0.25 | Cys | 0.25 | Cys | 0.25 | Cys | 0.25 | Cys | 0.25 | Cys | 0.25 |
125 | Thr | 0.45 | Thr |
0.65
| Thr |
0.9
| Thr | 0.45 | Thr | 0.45 | Thr | 0.45 | Thr | 0.45 | Thr | 0.45 | Thr | 0.45 |
126 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 | Thr | 0.25 |
127 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 | Pro | 0.4 |
128 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 | Ala | 0.8 |
129 | Gln | 0.8 | Gln | 0.8 | Gln |
0.4
| Gln | 0.8 | Gln | 0.8 | Gln | 0.8 | Gln | 0.8 | Gln | 0.8 | Gln | 0.8 |
130 | Gly | 0.65 | Gly | 0.65 | Gly | 0.65 | Gly | 0.65 | Gly |
0.25
| Gly |
0.8
| Gly |
0.25
| Gly | 0.65 | Gly | 0.65 |
131 | Thr | 0.35 | Thr | 0.35 | Thr | 0.35 | Thr | 0.35 | Thr |
-0.05
| Thr | 0.35 | Thr |
-0.05
| Thr | 0.35 | Thr | 0.35 |
132 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser |
-0.05
| Ser |
0.65
| Ser |
-0.05
| Ser | 0.35 | Ser | 0.35 |
133
| Met | -0.2 | Met | -0.2 | Met | -0.2 |
Leu
|
-0.05
|
Ile
|
-0.6
|
Thr
|
0.8
|
Val
|
-0.45
| Met | -0.2 | Met | -0.2 |
134 | Phe | -0.2 | Phe | -0.2 | Phe | -0.2 | Phe | -0.2 | Phe | -0.2 | Phe |
-0.05
| Phe | -0.2 | Phe | -0.2 | Phe | -0.2 |
135 | Pro | 0.2 | Pro | 0.2 | Pro | 0.2 | Pro | 0.2 | Pro | 0.2 | Pro |
0.35
| Pro | 0.2 | Pro | 0.2 | Pro | 0.2 |
136 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 | Ser | 0.2 |
137 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 | Cys | 0.2 |
138 | Cys | 0.64 | Cys | 0.64 | Cys | 0.64 | Cys | 0.64 | Cys | 0.64 | Cys | 0.64 | Cys | 0.64 | Cys |
0.3
| Cys |
0.3
|
139 | Cys | 1.18 | Cys | 1.18 | Cys | 1.18 | Cys | 1.18 | Cys | 1.18 | Cys | 1.18 | Cys | 1.18 | Cys |
0.5
| Cys |
0.8
|
140 | Thr | 1.27 | Thr | 1.27 | Thr | 1.27 | Thr | 1.27 | Thr |
1.67
| Thr | 1.27 | Thr | 1.27 | Thr |
0.41
| Thr |
0.7
|
141 | Lys | 2.36 | Lys | 2.36 | Lys | 2.36 | Lys | 2.36 | Lys | 2.36 | Lys | 2.36 | Lys | 2.36 | Lys |
1.47
| Lys |
1.75
|
142 | Pro | 3.4 | Pro | 3.4 | Pro | 3.4 | Pro | 3.4 | Pro | 3.4 | Pro | 3.4 | Pro | 3.4 | Pro |
2.33
| Pro |
2.6
|
143
| Thr | 2.86 | Thr | 2.86 | Thr | 2.86 | Thr | 2.86 | Thr | 2.86 | Thr | 2.86 | Thr | 2.86 |
Met
|
2.74
|
Leu
|
3
|
144 | Asp | 2.57 | Asp | 2.57 | Asp | 2.57 | Asp | 2.57 | Asp | 2.57 | Asp | 2.57 | Asp | 2.57 | Asp |
3.1
| Asp |
2.45
|
145 | Gly | 1.93 | Gly | 1.93 | Gly | 1.93 | Gly | 1.93 | Gly | 1.93 | Gly | 1.93 | Gly | 1.93 | Gly |
2.49
| Gly |
2.15
|
146 | Asn | 1.59 | Asn | 1.59 | Asn | 1.59 | Asn | 1.59 | Asn | 1.59 | Asn | 1.59 | Asn | 1.59 | Asn |
1.58
| Asn |
1.25
|
147 | Cys | 0.1 | Cys | 0.1 | Cys | 0.1 | Cys | 0.1 | Cys | 0.1 | Cys | 0.1 | Cys | 0.1 | Cys |
0.72
| Cys |
0.4
|
148 | Thr | -0.6 | Thr | -0.6 | Thr | -0.6 | Thr | -0.6 | Thr | -0.6 | Thr | -0.6 | Thr | -0.6 | Thr |
-0.29
| Thr | -0.6 |
149 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 | Cys | -0.6 |
150 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 | Ile | -0.6 |
151 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 | Pro | -0.6 |
152 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 | Ile | -0.45 |
153 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 | Pro | -0.05 |
154 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 |
155 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 | Ser | 0.35 |
156 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 | Trp | -0.2 |
157 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 |
158 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 | Phe | -0.6 |
159 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 | Ala | -0.6 |
160 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 | Lys | -0.6 |