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Inflammation

Erschienen in:

01.06.2015

Preventive Effects of Protocatechuic Acid on LPS-Induced Inflammatory Response in Human Gingival Fibroblasts via Activating PPAR-γ

verfasst von: Yimin Wang, Jun Zhou, Sheng Fu, Cheng Wang, Bing Zhou

Erschienen in: Inflammation | Ausgabe 3/2015

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Abstract

Protocatechuic acid (PCA), a major metabolite of anthocyanins, has been shown to have antioxidant, antitumoral, and anti-inflammatory activities. In this study, we investigated the anti-inflammatory effects of PCA on lipopolysaccharide (LPS)-induced inflammatory response in human gingival fibroblasts (HGFs). The effects of PCA on LPS-induced inflammatory cytokines IL-6 and chemokines IL-8 in HGFs were detected by ELISA. The expression of NF-κB and PPAR-γ was detected by Western blot analysis. Our results demonstrated that PCA suppressed IL-6 and IL-8 production in LPS-stimulated HGFs. We also found that PCA inhibited LPS-induced NF-κB activation. Furthermore, the inhibition of PCA on LPS-induced IL-6 and IL-8 production can be reversed by PPAR-γ antagonist GW9662. In conclusion, the anti-inflammatory mechanism of PCA is associated with activating PPAR-γ, thereby inhibiting LPS-induced inflammatory response.

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Titel: Preventive Effects of Protocatechuic Acid on LPS-Induced Inflammatory Response in Human Gingival Fibroblasts via Activating PPAR-γ
verfasst von: Yimin Wang
Jun Zhou
Sheng Fu
Cheng Wang
Bing Zhou
Publikationsdatum: 01.06.2015
Verlag: Springer US
Erschienen in: Inflammation / Ausgabe 3/2015
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-014-0073-1

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