Background
Methods
Cell culture
Flow cytometry
Statistics
Results
The distribution of cells in different cell cycle phases (%)1
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
A549 | FaDu | H1299 | ||||||||||
<2C2
| G0 + 1
| S | G2 + M | <2C | G0 + 1
| S | G2 + M | <2C | G0 + 1
| S | G2 + M | |
5-fluorouracil (μM) | ||||||||||||
1 | 2 ± 1 | 62 ± 2 | 27 ± 1 | 11 ± 1 | 2 ± 1 | 51 ± 3 | 31 ± 2 | 16 ± 1 | 2 ± 1 | 56 ± 2 | 31 ± 2 | 15 ± 1 |
3 | 2 ± 1 | 57 ± 2 |
33 ± 1
| 10 ± 1 | 2 ± 1 |
55 ± 3
|
26 ± 2
| 19 ± 1 | 2 ± 1 |
52 ± 2
|
34 ± 2
| 14 ± 1 |
10 | 2 ± 1 |
31 ± 2
|
61 ± 2
| 8 ± 2 | 2 ± 1 |
28 ± 1
|
52 ± 1
| 20 ± 1 | 2 ± 1 |
52 ± 2
|
32 ± 2
| 16 ± 2 |
30 | 3 ± 1 |
46 ± 2
|
47 ± 2
| 7 ± 2 | 3 ± 1 | 48 ± 2 |
44 ± 1
| 18 ± 1 | 5 ± 1 |
50 ± 2
|
33 ± 2
| 17 ± 2 |
Camptothecin (nM) | ||||||||||||
10 | 2 ± 1 |
42 ± 4
| 24 ± 2 |
28 ± 3
| 6 ± 2 |
18 ± 1
|
24 ± 2
|
58 ± 3
| 2 ± 1 |
17 ± 4
|
40 ± 9
|
43 ± 9
|
30 |
8 ± 2
|
16 ± 2
|
68 ± 4
| 16 ± 4 |
10 ± 3
|
6 ± 1
|
77 ± 3
| 17 ± 3 |
12 ± 2
|
11 ± 3
|
69 ± 3
| 19 ± 2 |
100 |
32 ± 4
|
14 ± 2
|
69 ± 4
| 17 ± 2 |
17 ± 2
|
21 ± 3
|
68 ± 4
|
11 ± 1
|
17 ± 3
|
22 ± 1
|
56 ± 4
|
22 ± 3
|
300 |
45 ± 6
|
45 ± 4
|
45 ± 4
| 10 ± 2 |
15 ± 3
|
40 ± 3
|
50 ± 3
|
10 ± 2
|
15 ± 2
|
35 ± 3
|
40 ± 4
|
25 ± 2
|
Nutlin-3 (μM) | ||||||||||||
1 | 2 ± 1 | 61 ± 2 | 28 ± 2 | 11 ± 1 | 2 ± 1 | 40 ± 4 | 38 ± 3 | 22 ± 3 | 2 ± 2 | 57 ± 1 | 29 ± 2 | 14 ± 1 |
3 | 2 ± 1 |
71 ± 2
|
13 ± 1
|
16 ± 1
| 2 ± 1 | 44 ± 2 | 34 ± 2 | 22 ± 2 | 2 ± 1 | 57 ± 3 | 25 ± 2 | 18 ± 2 |
10 | 2 ± 1 |
69 ± 1
|
7 ± 3
|
24 ± 3
| 4 ± 1 | 45 ± 3 | 34 ± 2 | 21 ± 2 | 4 ± 1 | 54 ± 4 | 27 ± 4 | 19 ± 2 |
30 |
5 ± 1
|
68 ± 2
|
6 ± 1
|
26 ± 2
|
8 ± 1
|
48 ± 3
|
32 ± 2
| 20 ± 2 |
7 ± 1
| 63 ± 2 |
19 ± 2
| 18 ± 1 |
Roscovitine (μM) | ||||||||||||
1 | 2 ± 1 | 60 ± 2 | 24 ± 2 | 16 ± 1 | 2 ± 1 | 41 ± 4 | 39 ± 4 | 20 ± 2 | 2 ± 1 |
47 ± 1
|
36 ± 3
| 17 ± 2 |
3 | 2 ± 1 | 59 ± 4 | 20 ± 3 |
21 ± 1
| 2 ± 1 | 43 ± 1 | 32 ± 1 |
25 ± 1
| 2 ± 1 |
52 ± 1
|
33 ± 1
| 15 ± 2 |
10 | 5 ± 1 | 59 ± 4 | 20 ± 3 |
21 ± 1
| 4 ± 1 |
38 ± 2
|
27 ± 2
|
35 ± 2
| 4 ± 2 |
49 ± 2
| 29 ± 1 |
22 ± 1
|
30 |
10 ± 4
|
51 ± 5
| 24 ± 3 |
24 ± 3
|
10 ± 3
|
34 ± 3
| 30 ± 2 |
36 ± 2
|
11 ± 4
|
43 ± 3
| 26 ± 2 |
31 ± 3
|
Taxol (nM) | ||||||||||||
1 |
9 ± 2
| 60 ± 4 | 25 ± 4 | 15 ± 2 | 21 ± 2 | 39 ± 3 | 34 ± 6 | 27 ± 4 |
13 ± 6
| 48 ± 6 | 30 ± 3 | 22 ± 5 |
3 |
36 ± 5
|
41 ± 3
| 32 ± 5 |
27 ± 2
|
30 ± 5
|
22 ± 4
| 35 ± 5 |
43 ± 7
|
35 ± 6
|
40 ± 1
|
40 ± 1
|
20 ± 1
|
10 |
35 ± 6
|
22 ± 4
| 29 ± 3 |
51 ± 6
|
33 ± 6
|
12 ± 2
|
19 ± 4
|
69 ± 4
|
42 ± 5
|
34 ± 4
|
34 ± 4
|
32 ± 7
|
30 |
16 ± 4
|
15 ± 2
|
17 ± 3
|
68 ± 5
|
16 ± 3
|
4 ± 1
|
11 ± 2
|
85 ± 2
|
44 ± 4
|
18 ± 1
|
36 ± 4
|
46 ± 7
|
100 |
10 ± 2
|
18 ± 4
|
18 ± 3
|
64 ± 5
|
8 ± 2
|
5 ± 1
|
9 ± 3
|
87 ± 3
|
30 ± 4
|
21 ± 3
| 23 ± 3 |
55 ± 2
|
Control | ||||||||||||
- 3
| 2 ± 1 | 62 ± 1 | 26 ± 1 | 12 ± 1 | 2 ± 1 | 46 ± 2 | 36 ± 1 | 18 ± 1 | 2 ± 1 | 59 ± 2 | 26 ± 2 | 15 ± 1 |