Background
Method
Animal and surgical procedure
Study groups
Operative procedure and technique
Experiment-1: inflammatory markers production
Experiment-2: Histopahological Assessment
Immunohistochemistry
Analysis of HSP
Analysis of IL-6
Analysis of MPO
Statistical analysis
Results
Histopathological and immunohistochemical evaluation
H&E | Grade | Ischemic control | Sham | Heparinized | High dose-heparinized |
P values
| ||||
---|---|---|---|---|---|---|---|---|---|---|
Number | % | Number | % | Number | % | Number | % | |||
Edema | 0 | 0 | 0 | 3 | 42.9 | 4 | 57.1 | 4 | 57.1 | 0.032 |
1 | 7 | 71.4 | 4 | 57.1 | 3 | 42.9 | 3 | 42.9 | ||
Inflammation | 1 | 4 | 57.1 | 7 | 100 | 6 | 85.7 | 7 | 100 | 0.012 |
2 | 3 | 42.9 | 0 | 0 | 1 | 14.3 | 0 | 0 | ||
Cell Degeneration | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 14.3 | <0.0001 |
1 | 0 | 0 | 7 | 100 | 6 | 85.7 | 6 | 85.7 | ||
2 | 6 | 85.7 | 0 | 0 | 1 | 14.3 | 0 | 0 | ||
3 | 1 | 14.3 | 0 | 0 | 0 | 0 | 0 | 0 | ||
Congestion | 0 | 0 | 0 | 1 | 14.3 | 0 | 0 | 0 | 0 | 0.124 |
1 | 3 | 42.9 | 6 | 85.7 | 6 | 85.7 | 7 | 100 | ||
2 | 3 | 42.9 | 0 | 0 | 1 | 14.3 | 0 | 0 | ||
3 | 1 | 14.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Grade | Ischemic control | Sham | heparinized | High dose heparinized |
P value
| |||||
---|---|---|---|---|---|---|---|---|---|---|
Number | % | Number | % | Number | % | Number | % | |||
MPO | 0 | 0 | 0 | 4 | 57.1 | 2 | 28.6 | 5 | 71.4 | 0.019 |
1 | 5 | 71.4 | 3 | 42.9 | 5 | 71.4 | 2 | 28.6 | ||
2 | 2 | 28.6 | 0 | 0 | 0 | 0 | 0 | 0 | ||
HSP | 0 | 0 | 0 | 4 | 57.1 | 2 | 28.6 | 2 | 28.6 | 0.050 |
1 | 5 | 71.4 | 3 | 42.9 | 5 | 71.4 | 5 | 71.4 | ||
2 | 2 | 28.6 | 0 | 0 | 0 | 0 | 0 | 0 | ||
IL-6 | 0 | 0 | 0 | 4 | 57.1 | 2 | 28.6 | 4 | 57.1 | 0.089 |
1 | 6 | 85.7 | 3 | 42.9 | 5 | 71.4 | 3 | 42.9 | ||
2 | 1 | 14.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Grade | High dose-heparinized | Sham | İschemic control | Heparinized |
P value
| |||||
---|---|---|---|---|---|---|---|---|---|---|
Number | % | Number | % | Number | % | Number | % | |||
Edema | 0 | 4 | 57.1 | 5 | 71.4 | 5 | 71.49 | 5 | 71.4 | 0.613 |
1 | 3 | 42.9 | 2 | 28.6 | 2 | 28.6 | 2 | 28.6 | ||
Inflammation | 0 | 3 | 42.9 | 5 | 71.4 | 3 | 42.9 | 5 | 71.4 | 0.499 |
1 | 4 | 57.1 | 2 | 28.6 | 4 | 57.1 | 2 | 28.6 | ||
Degeneration | 0 | 4 | 57.1 | 6 | 85.7 | 0 | 0 | 1 | 14.3 | 0.005 |
1 | 3 | 42.9 | 1 | 14.3 | 6 | 85.7 | 6 | 85.7 | ||
2 | 0 | 0 | 0 | 0 | 1 | 14.3 | 0 | 0 | ||
Congession | 0 | 4 | 57.1 | 5 | 71.4 | 6 | 85.7 | 6 | 85.7 | 0.558 |
1 | 3 | 42.9 | 2 | 28.6 | 1 | 14.3 | 1 | 14.3 |
Grade | Ischemic control | Sham | Heparinized | High dose-heparinized |
P value
| |||||
---|---|---|---|---|---|---|---|---|---|---|
Number | % | Number | % | Number | % | Number | % | |||
MPO | 0 | 0 | 0 | 4 | 57.1 | 4 | 57.1 | 4 | 57.1 | 0.023 |
1 | 7 | 100 | 3 | 42.9 | 3 | 42.9 | 3 | 42.9 | ||
HSP | 0 | 2 | 28.6 | 5 | 71.4 | 4 | 57.1 | 5 | 71.4 | 0.489 |
1 | 4 | 57.1 | 2 | 28.6 | 3 | 42.9 | 2 | 28.6 | ||
2 | 1 | 14.3 | 0 | 0 | 0 | 0 | 0 | 0 | ||
IL-6 | 0 | 1 | 14.3 | 4 | 57.1 | 3 | 42.9 | 5 | 71.4 | 0.143 |
1 | 6 | 85.7 | 3 | 42.9 | 4 | 57.1 | 2 | 28.6 |