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World Journal of Urology

Erschienen in:

01.02.2009 | Original Article

Protective effect of trapidil on long-term histologic damage in a rat model of testicular ischemia-reperfusion injury

verfasst von: Murat Bozlu, Deniz Acar, Selahittin Cayan, Savas Aktas, Ahmet Tunckiran

Erschienen in: World Journal of Urology | Ausgabe 1/2009

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Abstract

Objectives

Trapidil is an antianginal compound with a broad spectrum of pharmacological activities. In recent years, it has been used successfully to decrease ischemia-reperfusion injury in several organ systems. We evaluated the effect of trapidil on the long-term histologic damage in testicular ischemia-reperfusion injury.

Methods

Adult male Wistar rats were divided into three groups of six rats each. One group underwent 2 h of testicular torsion; one received pretreatment with trapidil before detorsion; and one group underwent sham operation. All rats underwent bilateral orchiectomy 60 days after the experiment. The mean seminiferous tubular diameter, germinal epithelial cell thickness, and mean testicular biopsy score were determined by histological examination of each testis.

Results

Testicular torsion–detorsion caused a significant decrease in the mean seminiferous tubular diameter, germinal epithelial cell thickness, and mean testicular biopsy score in the ipsilateral testes, but not in the contralateral testes. The animals treated with trapidil had a significant increase in these histological parameters as compared to the torsion–detorsion group.

Conclusion

Trapidil administration before reperfusion may have the potential to decrease the long-term histologic damage that occurs after experimental testicular torsion. Trapidil is used as an antianginal drug and additional clinical studies are required to elucidate the protective role of trapidil in patients with testicular torsion.

Visser AJ, Heyns CF (2003) Testicular function after torsion of the spermatic cord. BJU Int 92:200–203PubMedCrossRef

Cuzzocrea S, Riley DP, Caputi AP, Salvemini D (2001) Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev 53:135–159PubMed

Lysiak JJ, Nguyen QA, Kirby JL, Turner TT (2003) Ischemia-reperfusion of the murine testis stimulates the expression of proinflammatory cytokines and activation of c-jun N-terminal kinase in a pathway to E-selectin expression. Biol Reprod 69:202–210PubMedCrossRef

Maresta A, Balducelli M, Cantini L, Casari A, Chioin R, Fabbri M, Fontanelli A, Monici Preti PA, Repetto S, De Servi S (1994) Trapidil (triazolopyrimidine), platelet-derived growth factor antagonist, reduced restenosis after percutaneous transluminal coronary angioplasty: Results of the randomized, double-blind STARC study. Circulation 90:2710–2715PubMed

Avlan D, Tamer L, Ayaz L, Polat A, Ozturk C, Ozturhan H, Camdeviren H, Aksoyek S (2006) Effects of trapidil on renal ischemia-reperfusion injury. J Pediatr Surg 41:1686–1693PubMedCrossRef

Gocer AI, Ildan F, Tuna M, Polat S, Tamer L, Erman T, Kaya M (2001) Effects of trapidil on ATPase, lipid peroxidation, and correlation with ultrastructure in experimental spinal cord injury. Neurosurg Rev 24:136–142PubMedCrossRef

Bagdatoglu C, Saray A, Surucu HS, Ozturk H, Tamer L (2002) Effect of trapidil in ischemia/reperfusion injury of peripheral nerves. Neurosurgery 51:212–220PubMedCrossRef

Colak T, Polat A, Bagdatoglu O, Kanik A, Turkmenoglu O, Aydin S (2003) Effect of trapidil in ischemia/reperfusion injury on rat small intestine. J Invest Surg 16:167–176PubMedCrossRef

Somuncu S, Cakmak M, Erdogan S, Caglayan O, Akman H, Kaya M (2006) Protective effects of trapidil in ischemia-reperfusion injury due to testicular torsion and detorsion: an experimental study. Int J Urol 13:601–605PubMedCrossRef

10.

Bozlu M, Eskandari G, Çayan S, Canpolat B, Akbay E, Atik U (2003) The effect of poly(adenosine diphosphate-ribose) polymerase inhibitors on biochemical changes in testicular ischemia-reperfusion injury. J Urol 169:1870–1873PubMedCrossRef

11.

Bozlu M, Coskun B, Cayan S, Acar D, Aktas S, Ulusoy E, Akbay E (2004) Inhibition of poly(adenosine diphosphate-ribose) polymerase decreases long-term histologic damage in testiscular ischemia-reperfusion injury. Urology 63:791–795PubMedCrossRef

12.

Tunckiran A, Cayan S, Bozlu M, Yilmaz N, Acar D, Akbay E (2005) Protective effect of vascular endothelial growth factor on histologic changes in testicular ischemia-reperfusion injury. Fertil Steril 84:468–473PubMedCrossRef

13.

Johnsen SG (1970) Testicular biopsy score count-A method for registration of spermatogenesis in human testis: normal values and result in 352 hypogonadal males. Hormones 1:2–25PubMedCrossRef

14.

Nieder J, Claus P, Auguntin W (1995) Effect of trapidil on the PGI₂ and TxA₂ synthesis in human umbilical veins perfused in vitro. Prostaglandins 49:311–318PubMedCrossRef

15.

Zhou L, Ismaili J, Stordeur P, Thielemans K, Goldman M, Pradier O (1999) Inhibition of the CD40 pathway of monocyte activation by triazolopyrimidine. Clin Immunol 93:232–238PubMedCrossRef

16.

Arap MA, Vicentini FC, Cocuzza M, Hallak J, Athayde K, Lucon AM, Arap S, Srougi M (2007) Late hormonal levels, semen parameters, and presence of antisperm antibodies in patients treated for testicular torsion. J Androl 28:528–532PubMedCrossRef

17.

Adivarekar PK, Bhagwat SS, Raghavan V, Bandivdekar AH (2005) Effect of Lomodex-MgSO(4) in the prevention of reperfusion injury following unilateral testicular torsion: an experimental study in rats. Pediatr Surg Int 21:184–190PubMedCrossRef

18.

Becker EJ Jr, Turner TT (1995) Endocrine and exocrine effects of testicular torsion in the prepubertal and adult rat. J Androl 16:342–351PubMed

19.

Payabvash S, Kiumehr S, Tavangar SM, Dehpour AR (2008) Ethyl pyruvate reduces germ cell-specific apoptosis and oxidative stress in rat model of testicular torsion/detorsion. J Pediatr Surg 43:705–712PubMedCrossRef

20.

Can C, Tore F, Tuncel N, Uysal O, Gurer F, Ak D, Tuncel M (2004) Protective effect of vasoactive intestinal peptide on testicular torsion-detorsion injury: association with heparin-containing mast cells. Urology 63:195–200PubMedCrossRef

21.

Prillaman HM, Turner TT (1997) Rescue of testicular function after acute experimental torsion. J Urol 157:340–345PubMedCrossRef

22.

Kehinde EO, Anim JT, Mojiminiyi OA, Al-Awadi F, Shihab-Eldeen A, Omu AE, Fatinikun T, Prasad A, Abraham M (2005) Allopurinol provides long-term protection for experimentally induced testicular torsion in a rabbit model. BJU Int 96:175–180PubMedCrossRef

23.

Lysiak JJ, Nguyen QA, Turner TT (2002) Peptide and nonpeptide reactive oxygen scavengers provide partial rescue of the testis after torsion. J Androl 23:400–409PubMed

24.

Turner TT, Tung KS, Tomomasa H, Wilson LW (1997) Acute testicular ischemia results in germ cell-specific apoptosis in the rat. Biol Reprod 57:1267–1274PubMedCrossRef

25.

Savas C, Ozogul C, Karaoz E, Bezir M (2002) Ischemia, whether from ligation or torsion, causes ultrastructural changes on the contralateral testis. Scand J Urol Nephrol 36:302–306PubMedCrossRef

26.

Uz E, Sogut S, Sahin S, Var A, Ozyurt H, Gulec M, Akyol O (2002) The protective role of caffeic acid phenethyl ester (CAPE) on testicular tissue after testicular torsion and detorsion. World J Urol 20:264–270PubMed

27.

Unsal A, Devrim E, Guven C, Eroglu M, Durak I, Bozoklu A, Balbay MD (2004) Propofol attenuates reperfusion injury after testicular torsion and detorsion. World J Urol 22:461–465PubMedCrossRef

28.

Yazihan N, Ataoglu H, Koku N, Erdemli E, Sargin AK (2007) Protective role of erythropoietin during testicular torsion of the rats. World J Urol 25:531–536PubMedCrossRef

29.

Beheshtian A, Salmasi AH, Payabvash S, Kiumehr S, Ghazinezami B, Rahimpour S, Tavangar SM, Dehpour AR (2008) Protective effects of sildenafil administration on testicular torsion/detorsion damage in rats. World J Urol 26:197–202PubMedCrossRef

30.

Turner TT (1987) On unilateral testicular and epidymal torsion: no effect on the contralateral testis. J Urol 138:1285–1290PubMed

Titel: Protective effect of trapidil on long-term histologic damage in a rat model of testicular ischemia-reperfusion injury
verfasst von: Murat Bozlu
Deniz Acar
Selahittin Cayan
Savas Aktas
Ahmet Tunckiran
Publikationsdatum: 01.02.2009
Verlag: Springer-Verlag
Erschienen in: World Journal of Urology / Ausgabe 1/2009
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-008-0323-7

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