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08.11.2019 | Original Article

Proteomic and phosphoproteomic analyses reveal several events involved in the early stages of bovine herpesvirus 1 infection

verfasst von: Marcos J. Magalhães-Junior, Maria Cristina Baracat-Pereira, Lorena K. J. Pereira, Camilo E. Vital, Marcus R. Santos, Pricila S. Cunha, Kenner M. Fernandes, Gustavo C. Bressan, Juliana L. R. Fietto, Abelardo Silva-Júnior, Márcia R. Almeida

Erschienen in: Archives of Virology | Ausgabe 1/2020

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Abstract

Herpesviruses are predicted to express more than 80 proteins during their infection cycle. The proteins synthesized by the immediate early genes and early genes target signaling pathways in host cells that are essential for the successful initiation of a productive infection and for latency. In this study, proteomic and phosphoproteomic tools showed the occurrence of changes in Madin-Darby bovine kidney cells at the early stage of the infection by bovine herpesvirus 1 (BoHV-1). Proteins that had already been described in the early stage of infection for other herpesviruses but not for BoHV-1 were found. For example, stathmin phosphorylation at the initial stage of infection is described for the first time. In addition, two proteins that had not been described yet in the early stages of herpesvirus infections in general were ribonuclease/angiogenin inhibitor and Rab GDP dissociation inhibitor beta. The biological processes involved in these cellular responses were repair and replication of DNA, splicing, microtubule dynamics, and inflammatory responses. These results reveal pathways that might be used as targets for designing antiviral molecules against BoHV-1 infection.

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Titel: Proteomic and phosphoproteomic analyses reveal several events involved in the early stages of bovine herpesvirus 1 infection
verfasst von: Marcos J. Magalhães-Junior
Maria Cristina Baracat-Pereira
Lorena K. J. Pereira
Camilo E. Vital
Marcus R. Santos
Pricila S. Cunha
Kenner M. Fernandes
Gustavo C. Bressan
Juliana L. R. Fietto
Abelardo Silva-Júnior
Márcia R. Almeida
Publikationsdatum: 08.11.2019
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 1/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04452-1

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