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Medical Oncology

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01.06.2016 | Original Paper

Rap2B promotes cell proliferation, migration and invasion in prostate cancer

verfasst von: Jiehui Di, Huan Cao, Juangjuan Tang, Zheng Lu, Keyu Gao, Zhesi Zhu, Junnian Zheng

Erschienen in: Medical Oncology | Ausgabe 6/2016

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Abstract

Rap2B, a member of the Ras family of small GTP-binding proteins, reportedly presents a high level of expression in various human tumors and plays a significant role in the development of tumor. However, the function of Rap2B in prostate cancer (PCa) remains unclear. We elucidated the stimulative role of Rap2B in PCa cell proliferation, migration and invasion by means of the CCK-8 cell proliferation assay, cell cycle analysis and transwell migration assay. Western blot analysis uncovered that elevated Rap2B leads to increased phosphorylation levels of FAK, suggesting that FAK-dependent pathway might be responsible for the effect of Rap2B on PCa cells migration and invasion. Inversely, FAK-specific inhibitor (PF-573228) can abort Rap2B-induced FAK phosphorylation. In vivo experiment confirmed that Rap2B positively regulated PCa growth and metastasis, as well as the expression of phosphorylated FAK. Collectively, these findings shed light on Rap2B as a potential therapeutic target for PCa.

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Titel: Rap2B promotes cell proliferation, migration and invasion in prostate cancer
verfasst von: Jiehui Di
Huan Cao
Juangjuan Tang
Zheng Lu
Keyu Gao
Zhesi Zhu
Junnian Zheng
Publikationsdatum: 01.06.2016
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 6/2016
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-016-0771-7

Springer Medizin

Rap2B promotes cell proliferation, migration and invasion in prostate cancer

Abstract

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