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Journal of Cancer Research and Clinical Oncology

Erschienen in:

01.10.2015 | Original Article – Cancer Research

p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading

verfasst von: Jiehui Di, Hui Huang, Yan Wang, Debao Qu, Juanjuan Tang, Qian Cheng, Zheng Lu, Yanping Zhang, Junnian Zheng

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 10/2015

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Abstract

Purpose

Cell migration requires spatiotemporal integration of signals that target cytoskeletal. Previous studies have indicated that Rho GTPases are crucial regulators of actin dynamics. As homologs of Rho proteins, the role of Rap2B in the regulation of cytoskeleton and its cell signaling pathway remains unknown.

Methods

The cellular functions of Rap2B were monitored by Western blotting and immunofluorescence staining in order to characterize the protein level and the cell shape.

Results

Here, we show that expression of Rap2B was induced by nocodazole in a p53-dependent manner. However, Rap2B itself is not necessary for p53-dependent cell cycle arrest. We evidenced that over-expression of Rap2B may inhibit cell spreading by disrupting actin dynamics upon nocodazole treatment, but Rap2B (C180A) mutant does not. In contrast, knockdown of Rap2B promoted cell spreading.

Conclusions

Altogether, these results revealed that Rap2B plays a pivotal role in cytoskeleton reorganization and subsequently inhibits cell spreading, which could be responsible for cancer metastasis.

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Titel: p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading
verfasst von: Jiehui Di
Hui Huang
Yan Wang
Debao Qu
Juanjuan Tang
Qian Cheng
Zheng Lu
Yanping Zhang
Junnian Zheng
Publikationsdatum: 01.10.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 10/2015
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-015-1948-8

Springer Medizin

p53 target gene Rap2B regulates the cytoskeleton and inhibits cell spreading