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European Journal of Nutrition

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01.08.2015 | Original Contribution

Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil

verfasst von: Paola Priore, Donatella Caruso, Luisa Siculella, Gabriele V. Gnoni

Erschienen in: European Journal of Nutrition | Ausgabe 5/2015

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Abstract

Purpose

Regular consumption of extra virgin olive oil (EVOO) is associated with a low incidence of atherosclerotic diseases. The phenolic component contributes to the hypolipidemic action of EVOO, although the biochemical mechanisms leading this beneficial outcome are not fully understood. Since liver plays a pivotal role in the whole body lipid homeostasis, we investigated the short-term effects of EVOO extract, with a high phenol content (HPE), on lipid synthesis in primary rat hepatocytes. Refined olive oil extract, with a low phenol content, was used throughout this study as a control.

Methods

Olive oil phenols isolated with methanolic extractions were subsequently analyzed by high performance liquid chromatography, electrospray ionization tandem mass spectrometry, and gas chromatography mass spectrometry. Rat hepatocytes were obtained from collagenase perfusion of liver. A colorimetric assay was performed to exclude cytotoxicity of the extracts. Radioenzymatic methods were used in order to investigate hepatic lipid metabolism.

Results

HPE, dose- (0.1–50 μg/mL) and time-dependently (0.5–4 h) inhibited both lipogenesis and cholesterogenesis (n = 6, P < 0.05), as well as triglycerides synthesis (n = 5, P < 0.05). We showed that these effects are attributable to a short-term modulation by HPE of the key enzymes implicated in the abovementioned pathways (n = 5, P < 0.05).

Conclusions

The decrease in hepatic lipid synthesis may represent a potential mechanism underlying the hypolipidemic effect of EVOO phenols.

Tijburg LB, Mattern T, Folts JD, Weisgerber UM, Katan MB (1997) Tea flavonoids and cardiovascular disease: a review. Crit Rev Food Sci Nutr 37:771–785CrossRef

Covas MI, Konstantinidou V, Fitó M (2009) Olive oil and cardiovascular health. J Cardiovasc Pharmacol 54:477–482CrossRef

Yusuf S, Hawken S, Ounpuu S, Dans T, Avezum A, Lanas F, McQueen M, Budaj A, Pais P, Varigos J, Lisheng L (2004) Effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries (the INTERHEART study): case-control study. Lancet 364:937–952CrossRef

Galli C, Visioli F (1999) Antioxidant and other activities of phenolics in olives/olive oil, typical components of the Mediterranean diet. Lipids 34:S23–S26CrossRef

Visioli F, Poli A, Galli C (2002) Antioxidant and other biological activities of phenols from olives and olive oil. Med Res Rev 22:65–75CrossRef

Visioli F, Galli C (2002) Biological properties of olive oil phytochemicals. Crit Rev Food Sci Nutr 42:209–221CrossRef

Pérez-Jiménez F, Ruano J, Perez-Martinez P, Lopez-Segura F, Lopez-Miranda J (2007) The influence of olive oil on human health: not a question of fat alone. Mol Nutr Food Res 51:1199–1208CrossRef

Boskou D (2000) Mediterranean diets. In: Visioli F, Simopoulos AP (eds) World review nutrition and diet, vol 87. Karger, Basel, pp 56–77

Fragaki G, Spyros A, Siragakis G, Salivaras E, Dais P (2005) Detection of extra virgin olive oil adulteration with lampante olive oil and refined olive oil using nuclear magnetic resonance spectroscopy and multivariate statistical analysis. J Agric Food Chem 53:2810–2816CrossRef

10.

Weinbrenner T, Fitó M, de la Torre R, Saez GT, Rijken P, Tormos C, Coolen S, Albaladejo MF, Abanades S, Schroder H, Marrugat J, Covas MI (2004) Olive oils high in phenolic compounds modulate oxidative/antioxidative status in men. J Nutr 134:2314–2321

11.

Coni E, Di Benedetto R, Di Pasquale M, Roberta M, Modesti D, Mattei R, Carlini EA (2000) Protective effect of oleuropein, an olive oil biophenol, on low density lipoprotein oxidizability in rabbits. Lipids 35:45–54CrossRef

12.

Dell’Agli M, Maschi O, Galli GV, Fagnani R, Dal Cero E, Caruso D, Bosisio E (2008) Inhibition of platelet aggregation by olive oil phenols via cAMP-phosphodiesterase. Br J Nutr 99:945–951

13.

Petroni A, Blasevich M, Salami M, Papini N, Montedero GF, Galli C (1995) Inhibition of platelet aggregation and eicosanoid production by phenolic components of olive oil. Thromb Res 78:151–160CrossRef

14.

De la Puerta R, Ruiz-Gutierrez V, Hoult JR (1999) Inhibition of leukocyte 5-lipoxygenase by phenolics from virgin olive oil. Biochem Pharmacol 57:445–449CrossRef

15.

Carluccio MA, Siculella L, Ancora MA, Massaro M, Scoditti E, Storelli C, Visioli F, Distante A, De Caterina R (2003) Olive oil and red wine antioxidant polyphenols inhibit endothelial activation: antiatherogenic properties of Mediterranean diet phytochemicals. Arterioscler Thromb Vasc Biol 23:622–629CrossRef

16.

Carluccio MA, Massaro M, Scoditti E, De Caterina R (2007) Vasculoprotective potential of olive oil components. Mol Nutr Food Res 51:1225–1234CrossRef

17.

Jemai H, Bouaziz M, Fki I, El Feki A, Sayadi S (2008) Hypolipidemic and antioxidant activities of oleuropein and its hydrolysis derivative-rich extracts from Chemlali olive leaves. Chem Biol Interact 176:88–98CrossRef

18.

Covas MI, Nyyssönen K, Poulsen HE, Kaikkonen J, Zunft HJ, Kiesewetter H, Gaddi A, de la Torre R, Mursu J, Bäumler H, Nascetti S, Salonen JT, Fitó M, Virtanen J, Marrugat J (2006) EUROLIVE Study Group. The effect of polyphenols in olive oil on heart disease risk factors: a randomized trial. Ann Intern Med 145:333–341CrossRef

19.

Visioli F, Caruso D, Grande S, Bosisio R, Villa M, Galli G, Sirtori C, Galli C (2005) Virgin Olive Oil Study (VOLOS): vasoprotective potential of extra virgin olive oil in mildly dyslipidemic patients. Eur J Nutr 44:121–127CrossRef

20.

Ramirez-Tortosa MC, Urbano G, Lopez-Jurado M, Nestares T, Gomez MC, Mir A, Ros E, Mataix J, Gil A (1999) Extra-virgin olive oil increases the resistance of LDL to oxidation more than refined olive oil in free-living men with peripheral vascular disease. J Nutr 129:2177–2183

21.

Priore P, Sicullella L, Gnoni GV (2014) Extra virgin olive oil phenols down-regulate lipid synthesis in primary-cultured rat-hepatocytes. J Nutr Biochem 25:683–691CrossRef

22.

Del Coco L, Schena FP, Fanizzi FP (2012) 1H nuclear magnetic resonance study of olive oils commercially available as Italian products in the United States of America. Nutrients 4:343–355CrossRef

23.

Montedoro GF, Servili M, Baldioli M, Miniati E (1992) Simple and hydrolysable phenolic compounds in virgin olive oil. 1. Their extraction, separation, and quantitative and semiquantitative evaluation by HPLC. J Agric Food Chem 40:1571–1576CrossRef

24.

Cardoso SM, Guyot S, Marnet N, Lopes-da-Silva JA, Renard CMGC, Coimbra MA (2005) Characterization of phenolic extracts from olive pulp and olive pomace by electrospray mass spectrometry. J Sci Food Agric 85:21–32CrossRef

25.

Visioli F, Galli C, Bornet F, Mattei A, Patelli R, Galli G, Caruso D (2000) Olive oil phenolics are dose-dependently absorbed in humans. FEBS Lett 468:159–160CrossRef

26.

Mangiullo R, Gnoni A, Leone A, Gnoni GV, Papa S, Zanotti F (2008) Structural and functional characterization of F(o)F(1)-ATP synthase on the extracellular surface of rat hepatocytes. Biochim Biophys Acta 1777:1326–1335CrossRef

27.

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRef

28.

Priore P, Giudetti AM, Natali F, Gnoni GV, Geelen MJH (2007) Metabolism and short-term metabolic effects of conjugated linoleic acids in rat hepatocytes. Biochim Biophys Acta 1771:1299–1307CrossRef

29.

Giudetti AM, Leo M, Geelen MJ, Gnoni GV (2005) Short-term stimulation of lipogenesis by 3,5-L-diiodothyronine in cultured rat hepatocytes. Endocrinology 146:3959–3966CrossRef

30.

Geelen MJH (2005) The use of digitonin-permeabilized mammalian cells for measuring enzyme activities in the course of studies on lipid metabolism. Anal Biochem 347:1–9CrossRef

31.

Natali F, Siculella L, Salvati S, Gnoni GV (2007) Oleic acid is a potent inhibitor of fatty acid and cholesterol synthesis in C6 glioma cells. J Lipid Res 48:1966–1975CrossRef

32.

Gnoni GV, Paglialonga G, Siculella L (2009) Quercetin inhibits fatty acid and triacylglycerol synthesis in rat-liver cells. Eur J Clin Invest 39:761–768CrossRef

33.

Gnoni GV, Paglialonga G (2009) Resveratrol inhibits fatty acid and triacylglycerol synthesis in rat hepatocytes. Eur J Clin Invest 39:211–218CrossRef

34.

Fabiani R, Sepporta MV, Mazza T, Rosignoli P, Fuccelli R, De Bartolomeo A, Crescimanno M, Taticchi A, Esposto S, Servili M, Morozzi G (2011) Influence of cultivar and concentration of selected phenolic constituents on the in vitro chemopreventive potential of olive oil extracts. J Agric Food Chem 59:8167–8174CrossRef

35.

Vissers MN, Zock PL, Katan MB (2004) Bioavailability and antioxidant effects of olive oil phenols in humans: a review. Eur J Clin Nutr 58:955–965CrossRef

36.

de la Torre R (2008) Bioavailability of olive oil phenolic compounds in humans. Inflammopharmacology 16:245–247CrossRef

37.

Dell’Agli M, Fagnani R, Galli GV, Maschi O, Gilardi F, Bellosta S, Crestani M, Bosisio E, De Fabiani E, Caruso D (2010) Olive oil phenols modulate the expression of metalloproteinase 9 in THP-1 cells by acting on nuclear factor-kappaB signaling. J Agric Food Chem 58:2246–2252CrossRef

38.

Tunstall-Pedoe H, Kuulasmaa K, Mahonen M, Tolonen H, Ruokokoski E, Amouyel P (1999) Contribution of trends in survival and coronary-event rates to changes in coronary heart disease mortality: 10-year results from 37 WHO MONICA Project populations. Lancet 353:1547–1557CrossRef

39.

De Lorgeril M, Salen P, Martin JL, Monjaud I, Delaye J, Mamelle N (1999) Mediterranean diet, traditional risk factors, and the rate of cardiovascular complications after myocardial infarction: final report of the Lyon Diet Heart Study. Circulation 99:779–785CrossRef

40.

Martín-Peláez S, Covas MI, Fitó M, Kušar A, Pravst I (2013) Health effects of olive oil polyphenols: recent advances and possibilities for the use of health claims. Mol Nutr Food Res 57:760–771CrossRef

41.

Cicerale S, Lucas L, Keast R (2010) Biological activities of phenolic compounds present in virgin olive oil. Int J Mol Sci 11:458–479CrossRef

42.

Gimeno E, Fitó M, Lamuela-Raventós RM, Castellote AI, Covas M, Farré M, de La Torre-Boronat MC, López-Sabater MC (2002) Effect of ingestion of virgin olive oil on human low-density lipoprotein composition. Eur J Clin Nutr 56:114–120CrossRef

43.

Davis RA, Hui TY (2001) 2000 George Lyman Duff Memorial Lecture: atherosclerosis is a liver disease of the heart. Arterioscler Thromb Vasc Biol 21:887–898CrossRef

44.

Verschuren WM, Jacobs DR, Bloemberg BP, Kromhout D, Menotti A, Aravanis C, Blackburn H, Buzina R, Dontas AS, Fidanza F, Karvonen MJ, Nedelijković S, Nissinen A, Toshima H (1995) Serum total cholesterol and long-term coronary heart disease mortality in different cultures. Twenty-five-year follow-up of the seven countries study. JAMA 274:131–136CrossRef

45.

Fitó M, de la Torre R, Covas MI (2007) Olive oil and oxidative stress. Mol Nutr Food Res 51:1215–1224

46.

Semenkovich CF (1997) Regulation of fatty acid synthase (FAS). Prog Lipid Res 36:43–53CrossRef

47.

Jemai H, Fki I, Bouaziz M, Bouallagui Z, El Feki A, Isoda H, Sayadi S (2008) Lipid-lowering and antioxidant effects of hydroxytyrosol and its triacetylated derivative recovered from olive tree leaves in cholesterol-fed rats. J Agric Food Chem 56:2630–2636CrossRef

48.

Park S, Choi Y, Um SJ, Yoon SK, Park T (2011) Oleuropein attenuates hepatic steatosis induced by high-fat diet in mice. J Hepatol 54:984–993CrossRef

49.

Mangge H, Becker K, Fuchs D, Gostner JM (2014) Antioxidants, inflammation and cardiovascular disease. World J Cardiol 6:462–477CrossRef

50.

Williams RJ, Spencer JP, Rice-Evans C (2004) Flavonoids: antioxidants or signalling molecules? Free Radic Biol Med 36:838–849CrossRef

Titel: Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil
verfasst von: Paola Priore
Donatella Caruso
Luisa Siculella
Gabriele V. Gnoni
Publikationsdatum: 01.08.2015
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nutrition / Ausgabe 5/2015
Print ISSN: 1436-6207
Elektronische ISSN: 1436-6215
DOI: https://doi.org/10.1007/s00394-014-0761-5

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Rapid down-regulation of hepatic lipid metabolism by phenolic fraction from extra virgin olive oil

Abstract

Purpose

Methods

Results

Conclusions

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