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Journal of Cardiovascular Translational Research

Erschienen in:

09.03.2017 | Original Article

Regulation of A-Kinase-Anchoring Protein 12 by Heat Shock Protein A12B to Prevent Ventricular Dysfunction Following Acute Myocardial Infarction in Diabetic Rats

verfasst von: Vaithinathan Selvaraju, Sumanth C Suresh, Mahesh Thirunavukkarasu, Jayakanthan Mannu, Jocelyn L.C. Foye, Premendu P Mathur, J. Alexander Palesty, Juan A. Sanchez, David W. McFadden, Nilanjana Maulik

Erschienen in: Journal of Cardiovascular Translational Research | Ausgabe 2/2017

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Abstract

We examined the effects of overexpressing HSPA12B on angiogenesis and myocardial function by intramyocardial administration of adenovirus encoding HSPA12B (Ad. HSPA12B) in a streptozotocin-induced diabetic rat subjected to myocardial infarction. Rats were divided randomly into six groups: control sham (CS) + Ad.LacZ, control myocardial infarction (CMI) + Ad.LacZ, control MI + Ad.HSPA12B, diabetic sham (DS) + Ad.LacZ, diabetic MI + Ad.LacZ and diabetic MI + Ad.HSPA12B. Following MI or sham surgery, the respective groups received either Ad.LacZ or Ad.HSPA12B via intramyocardial injections. We observed increased capillary and arteriolar density along with reduced fibrosis and preserved heart functions in DMI-AdHSPA12B compared to DMI-AdLacZ group. Western blot analysis demonstrated enhanced HSPA12B, vascular endothelial growth factor (VEGF), thioredoxin-1 (Trx-1) expression along with decreased expression of thioredoxin interacting protein (TXNIP) and A kinase anchoring protein 12 (AKAP12) in the DMI-AdHSPA12B compared to DMI-AdLacZ group. Our findings reveal that HSPA12B overexpression interacts with AKAP12 and downregulate TXNIP in diabetic rats following acute MI.

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Titel: Regulation of A-Kinase-Anchoring Protein 12 by Heat Shock Protein A12B to Prevent Ventricular Dysfunction Following Acute Myocardial Infarction in Diabetic Rats
verfasst von: Vaithinathan Selvaraju
Sumanth C Suresh
Mahesh Thirunavukkarasu
Jayakanthan Mannu
Jocelyn L.C. Foye
Premendu P Mathur
J. Alexander Palesty
Juan A. Sanchez
David W. McFadden
Nilanjana Maulik
Publikationsdatum: 09.03.2017
Verlag: Springer US
Erschienen in: Journal of Cardiovascular Translational Research / Ausgabe 2/2017
Print ISSN: 1937-5387
Elektronische ISSN: 1937-5395
DOI: https://doi.org/10.1007/s12265-017-9734-4

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