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Journal of Assisted Reproduction and Genetics

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01.11.2011 | Embryo Biology

Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection

verfasst von: Hiroyuki Tomari, Kou Honjou, Yumi Nagata, Toshitaka Horiuchi

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 11/2011

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Abstract

Purpose

To investigate the relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection (ICSI).

Methods

Zygotes that had cleaved to two-cell stage by 27 h post-ICSI were classified as early cleaving and the remainder as late cleaving. Meiotic spindle parameters previously imaged using the PolScope were compared between the two groups.

Results

Of 384 embryos, 163 were classed as early cleaving and 221 as late cleaving. The rate of blastocyst formation or pregnancy by Day 2 embryo transfer was significantly higher following early cleavage than after late cleavage (52.4% vs. 24.4% or 32.6% vs. 11.4%). Spindle areas (108.0 vs. 89.8 μm²), lengths (14.7 vs. 13.4 μm) and PolScope retardance were also significantly greater in the early cleaving group.

Conclusions

Meiotic spindle parameters determine the timing of the first zygotic cleavage and are strong indicators of human embryo developmental potential.

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Titel: Relationship between meiotic spindle characteristics in human oocytes and the timing of the first zygotic cleavage after intracytoplasmic sperm injection
verfasst von: Hiroyuki Tomari
Kou Honjou
Yumi Nagata
Toshitaka Horiuchi
Publikationsdatum: 01.11.2011
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 11/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9634-5

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