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Clinical Oral Investigations
Erschienen in: Clinical Oral Investigations 1/2018

25.02.2017 | Original Article

Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors

verfasst von: Natacha M. M. da Costa, Adriane S. de Siqueira, André L. R. Ribeiro, Maria S. da Silva Kataoka, Ruy G. Jaeger, Sérgio M. de Alves-Júnior, Andrew M. Smith, João de Jesus Viana Pinheiro

Erschienen in: Clinical Oral Investigations | Ausgabe 1/2018

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Abstract

Objectives

Odontogenic cysts and tumors are the most relevant lesions that affect the gnathic bones. These lesions have in common the formation of cystic areas and this common feature may suggest involvement of similar mechanisms. The hypoxia inducible factor 1 alpha (HIF-1α), a responsive protein to hypoxia and caspase-3, an irreversible apoptosis marker, may contribute to cyst formation. Thus, this study aimed to investigate the immunoexpression of these proteins in odontogenic cysts and tumors.

Material and methods

Twenty cases of ameloblastoma, keratocystic odontogenic tumor (KOT) (n = 20), radicular cyst (RC) (n = 18), dentigerous cyst (DC) (n = 11), calcifying cystic odontogenic tumor (n = 8), and dental follicle (DF) (n = 10) were used to investigate HIF-1α and caspase-3 expression in sequential serial cuts by immunohistochemistry.

Results

HIF-1α was overexpressed in RC, DC, and ameloblastoma when compared with DF. The basal and sometimes the lower suprabasal layer showed no or very low expression in DC, KOT, and ameloblastoma, the last also showing strong expression in solid epithelial areas and initial cystic formation regions. Caspase-3 was found to be overexpressed in all lesions, with the highest expression in odontogenic cysts compared to tumors. HIF-1α and caspase-3 were localized in similar areas of the same lesions, especially in the epithelium surrounding cystic formations.

Conclusions

This study showed distinct immunoexpression of HIF-1α and caspase-3 in odontogenic cyst and tumors, with higher expression observed in odontogenic cysts.

Clinical relevance

These findings suggest a possible correlation between hypoxia, apoptosis, and cystogenesis, leading to understand the mechanisms responsible to cystic formation in odontogenic lesions.
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Metadaten
Titel
Role of HIF-1α and CASPASE-3 in cystogenesis of odontogenic cysts and tumors
verfasst von
Natacha M. M. da Costa
Adriane S. de Siqueira
André L. R. Ribeiro
Maria S. da Silva Kataoka
Ruy G. Jaeger
Sérgio M. de Alves-Júnior
Andrew M. Smith
João de Jesus Viana Pinheiro
Publikationsdatum
25.02.2017
Verlag
Springer Berlin Heidelberg
Erschienen in
Clinical Oral Investigations / Ausgabe 1/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-017-2090-6

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