nach oben

International Journal of Hematology

Erschienen in:

07.07.2017 | Original Article

Screening novel autoantigens targeted by serum IgG autoantibodies in immunorelated pancytopenia by SEREX

verfasst von: Shanfeng Hao, Rong Fu, Huaquan Wang, Zonghong Shao

Erschienen in: International Journal of Hematology | Ausgabe 5/2017

Einloggen, um Zugang zu erhalten

Abstract

Immunorelated pancytopenia (IRP) is characterized by pancytopenia caused by autoantibody-mediated destruction or suppression of bone marrow. However, the autoantigens targeted by autoantibodies in IRP remain unclear. In the present study, we screened novel autoantigens in IRP by serological analysis of recombinant cDNA expression libraries and compared anti-UQCR10 antibody levels between IRP and normal controls detected by immunoblotting. Our results indicate that we successfully constructed the K562 cDNA library, which we used to screen seven candidate autoantigens expressed in haematopoietic cells of IRP: ferritin, light polypeptide, ubiquinol-cytochrome c reductase, complex III subunit X (UQCR10), multifunctional methyl-transferase subunit TRM112-like protein isoform 1 (TRMT112), hemoglobin gamma-G, stathmin 1 (STMN1), transcript variant 3, phosphoglycerate kinase 1 (PGK1), and trafficking protein particle complex subunit 4 (TRAPPC4). Six of 17 (35.29%) IRP patients exhibited positive reactivity to UQCR10 antigen, while only one of 10 (10%) of normal controls reacted to UQCR10 antigen. The IRP patients with positive reactivity to UQCR10 antigen exhibited significantly improved total efficiency (6/6) compared with those with negative reactivity (5/11). Thus, UQCR10 may be implicated as one of the autoantigens involved in development of IRP.

He H, Shao Z, Liu H, Song L, Tian P, Cao Z, et al. Immunorelated pancytopenia. Zhonghua Xue Ye Xue Za Zhi. 2001;22:79–82.PubMed

Yue LZ, Shao ZH. Research progress on the red cell diseases in China. Chin Med J (Engl). 2012;125:2746–51.

He H, Shao Z, Cao Z, Tian P, Cui W, Liu H, et al. Category of bone marrow mononuclear cells Coombs test. Zhonghua Xue Ye Xue Za Zhi. 2000;22:550.

Fu R, Shao ZH, Liu H, He H, Jia HR, Sun J, et al. Category, quantity and clinical significance of autoantibodies on bone marrow hematopoietic cells in patients with immunorelated cytopenia. Zhonghua Xue Ye Xue Za Zhi. 2003;24:177–80.PubMed

Wang YH, Fu R, Dong SW, Liu H, Shao ZH. Erythroblastic islands in the bone marrow of patients with immune-related pancytopenia. PLoS One. 2014;9:e95143.CrossRefPubMedPubMedCentral

Liu H, Fu R, Wang Y, Liu H, Li L, Wang H, et al. Detection and analysis of autoantigens targeted by autoantibodies in immunorelated pancytopenia. Clin Dev Immunol. 2013;2013:297678.PubMedPubMedCentral

Shao Y, Fu R, Liu H, Wang Y, Ding S, Wang H, et al. IgG autoantibody subclasses altered in immuno-related hemocytopenia. Cell Immunol. 2015;294:13–20.CrossRefPubMed

Fu R, Shao ZH, Liu H, Wu YH, Wang HQ, Xing LM. Role of B lymphocyte and its subpopulations in pathogenesis of immunorelated pancytopenia. Chin Med Sci J. 2007;22:199–202.PubMed

Fu R, Shao Z, He H, Liu H, Jia H, Sun J, et al. Quantity and apoptosis-related protein level of B lymphocyte in patients with immunorelated pancytopenia. Zhonghua Xue Ye Xue Za Zhi. 2002;23:236–8.PubMed

10.

Fu R, Shao ZH, Liu H, He H, Sun J, Zhao MF, et al. Proliferation of bone marrow hematopoietic stem cells and function of T helper lymphocytes of patients with immuno-related pancytopenia. Zhonghua Xue Ye Xue Za Zhi. 2004;25:213–6.PubMed

11.

Fu R, Wang HL, Chen J, Wang J, Li LJ, Liu H, et al. Study of the quantity and function of Th17 cells in the blood cytopenic patients with positive BMMNC-Coombs test. Zhonghua Xue Ye Xue Za Zhi. 2010;31:684–7.PubMed

12.

Fu R, Chen J, Wang HL, Wang J, Li LJ, Liu H, et al. Quantity and function of regulatory T cells in hemocytopenic patients with positive BMMNC-Coombs test. Zhonghua Yi Xue Za Zhi. 2010;90:2989–93.PubMed

13.

Teng GS, Fu R, Liu H, Wang HL, Wang YH, Ruan EB, et al. Expression of CD80 and CD86 on dendritic cells of patients with immune related pancytopenia and its clinical significance. Zhonghua Xue Ye Xue Za Zhi. 2012;33:865–8.PubMed

14.

Teng GS, Fu R, Liu H, Wang HL, Wang YH, Ruan EB, et al. Quantity and subtypes of dendritic cells in patients with immune related pancytopenia and their clinical significance. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2012;20:722–6.PubMed

15.

Yu H, Fu R, Wang YH, Wang HQ, Liu H, Li LJ, et al. Preliminary study on the quantity and function of T follicular helper cells in the cytopenic patients with positive BMMNC-Coombs test. Zhonghua Xue Ye Xue Za Zhi. 2013;34:606–9.PubMed

16.

Yu H, Zhang J, Fu R, Liu H, Wang H, Ding K, et al. Increased frequency of bone marrow T follicular helper cells in patients with immune-related pancytopenia. Clin Dev Immunol. 2013;2013:730450.PubMedPubMedCentral

17.

Wang YH, Fu R, Shao ZH. A pilot study of memory B lymphocytes in relapsed immune-related pancytopenia patients. Clin Lab. 2014;60:729–33.PubMed

18.

Wang YH, Fu R, Shao ZH, Wang HQ, Xing LM, Liu H, et al. Study on quantity and function of bone marrow macrophages in patients with BMMNC-Coombs Test(+) pancytopenia. Zhonghua Xue Ye Xue Za Zhi. 2009;30:538–42.PubMed

19.

Wang YH, Fu R, Shao ZH, Xing LM, Wang HQ, Wu YH, et al. Expression of bone marrow macrophages antigen activation and its clinical significance in pancytopenia patients with positive bone marrow mononuclear cells-Coombs test. Zhonghua Nei Ke Za Zhi. 2010;49:146–9.PubMed

20.

Chen J, Fu R, Li LJ, Liu H, Wang YH, Wang HL, et al. Variation in complement level and its significance in cytopenia patients with positive BMMNC-Coombs. Zhonghua Xue Ye Xue Za Zhi. 2009;30:454–7.PubMed

21.

Valent P, Horny HP, Bennett JM, Fonatsch C, Germing U, Greenberg P, et al. Definitions and standards in the diagnosis and treatment of the myelodysplastic syndromes: consensus statements and report from a working conference. Leuk Res. 2007;31:727–36.CrossRefPubMed

22.

Malcovati L, Cazzola M. The shadowlands of MDS: idiopathic cytopenias of undetermined significance (ICUS) and clonal hematopoiesis of indeterminate potential (CHIP). Hematology Am Soc Hematol Educ Program. 2015;2015:299–307.PubMed

23.

Schroeder T, Ruf L, Bernhardt A, Hildebrandt B, Aivado M, Aul C, et al. Distinguishing myelodysplastic syndromes (MDS) from idiopathic cytopenia of undetermined significance (ICUS): HUMARA unravels clonality in a subgroup of patients. Ann Oncol. 2010;21:2267–71.CrossRefPubMed

24.

Fu R, Liu H, Wang Y, Liu H, He H, Chen J, et al. Distinguishing immunorelated haemocytopenia from idiopathic cytopenia of undetermined significance (ICUS): a bone marrow abnormality mediated by autoantibodies. Clin Exp Immunol. 2014;177:412–8.CrossRefPubMedPubMedCentral

25.

Krebs P, Kurrer M, Sahin U, Tureci O, Ludewig B. Autoimmunity seen through the SEREX-scope. Autoimmun Rev. 2003;2:339–45.CrossRefPubMed

26.

Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS. A multipotential leukemia cell line (K-562) of human origin. Proc Soc Exp Biol Med. 1981;166:546–50.CrossRefPubMed

27.

Shao ZH, Fu R. Immuno-related pancytopenia: a newly identified disease. Natl Med J China. 2005;40:5–9.

28.

Schagger H, Brandt U, Gencic S, von Jagow G. Ubiquinol-cytochrome-c reductase from human and bovine mitochondria. Methods Enzymol. 1995;260:82–96.CrossRefPubMed

29.

Meunier B, Fisher N, Ransac S, Mazat JP, Brasseur G. Respiratory complex III dysfunction in humans and the use of yeast as a model organism to study mitochondrial myopathy and associated diseases. Biochim Biophys Acta. 2013;1827:1346–61.CrossRefPubMed

30.

Shichijo K, Mitsui T, Kunishige M, Kuroda Y, Masuda K, Matsumoto T. Involvement of mitochondria in myasthenia gravis complicated with dermatomyositis and rheumatoid arthritis: a case report. Acta Neuropathol. 2005;109:539–42.CrossRefPubMed

31.

Li X, Fang P, Mai J, Choi ET, Wang H, Yang XF. Targeting mitochondrial reactive oxygen species as novel therapy for inflammatory diseases and cancers. J Hematol Oncol. 2013;6:19.CrossRefPubMedPubMedCentral

32.

Sena LA, Li S, Jairaman A, Prakriya M, Ezponda T, Hildeman DA, et al. Mitochondria are required for antigen-specific T cell activation through reactive oxygen species signaling. Immunity. 2013;38:225–36.CrossRefPubMedPubMedCentral

33.

Pazmandi K, Agod Z, Kumar BV, Szabo A, Fekete T, Sogor V, et al. Oxidative modification enhances the immunostimulatory effects of extracellular mitochondrial DNA on plasmacytoid dendritic cells. Free Radic Biol Med. 2014;77:281–90.CrossRefPubMed

Titel: Screening novel autoantigens targeted by serum IgG autoantibodies in immunorelated pancytopenia by SEREX
verfasst von: Shanfeng Hao
Rong Fu
Huaquan Wang
Zonghong Shao
Publikationsdatum: 07.07.2017
Verlag: Springer Japan
Erschienen in: International Journal of Hematology / Ausgabe 5/2017
Print ISSN: 0925-5710
Elektronische ISSN: 1865-3774
DOI: https://doi.org/10.1007/s12185-017-2287-0

Neu im Fachgebiet Onkologie

25.04.2024 | Nierenkarzinom | Nachrichten

Springer Medizin

Screening novel autoantigens targeted by serum IgG autoantibodies in immunorelated pancytopenia by SEREX

Abstract

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 5/2017

MiR-155 enhances phagocytic activity of β-thalassemia/HbE monocytes via targeting of BACH1

Retrospective analysis of prognostic factors for Waldenstrӧm macroglobulinemia: a multicenter cooperative study in Japan

Efficacy and safety of full-length pegylated recombinant factor VIII with extended half-life in previously treated patients with hemophilia A: comparison of data between the general and Japanese study populations

Steroid-resistant autoimmune myelofibrosis in a patient with autoimmune hepatitis and Evans syndrome complicated with increased expression of TGF-β in the bone marrow: a case report

Romidepsin in Japanese patients with relapsed or refractory peripheral T-cell lymphoma: a phase I/II and pharmacokinetics study

Congenital immature pure erythroid leukemia with E-cadherin expression

Neu im Fachgebiet Onkologie

Adjuvante Immuntherapie verlängert Leben bei RCC

Alectinib verbessert krankheitsfreies Überleben bei ALK-positivem NSCLC

Bei Senioren mit Prostatakarzinom auf Anämie achten!

ICI-Therapie in der Schwangerschaft wird gut toleriert

Update Onkologie