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Erschienen in: Archives of Virology 12/2016

19.09.2016 | Brief Report

Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid

verfasst von: S. J. Harper, N. Killiny, S. Tatineni, S. Gowda, S. J. Cowell, T. Shilts, W. O. Dawson

Erschienen in: Archives of Virology | Ausgabe 12/2016

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Abstract

Vector transmission is an important part of the viral infection cycle, yet for many viruses little is known about this process, or how viral sequence variation affects transmission efficacy. Here we examined the effect of substituting genes from the highly transmissible FS577 isolate of citrus tristeza virus (CTV) in to the poorly transmissible T36-based infectious clone. We found that introducing p65 or p61 sequences from FS577 significantly increased transmission efficacy. Interestingly, replacement of both genes produced a greater increase than either gene alone, suggesting that CTV transmission requires the concerted action of co-evolved p65 and p61 proteins.
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Metadaten
Titel
Sequence variation in two genes determines the efficacy of transmission of citrus tristeza virus by the brown citrus aphid
verfasst von
S. J. Harper
N. Killiny
S. Tatineni
S. Gowda
S. J. Cowell
T. Shilts
W. O. Dawson
Publikationsdatum
19.09.2016
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 12/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-016-3070-x

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