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European Journal of Medical Research

Erschienen in:

01.12.2010 | Research

Spinning around or stagnation - what do osteoblasts and chondroblasts really like?

verfasst von: C Zilkens, T Lögters, B Bittersohl, R Krauspe, S Lensing-Höhn, M Jäger

Erschienen in: European Journal of Medical Research | Ausgabe 1/2010

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Abstract

Objective

The influcence of cytomechanical forces in cellular migration, proliferation and differentation of mesenchymal stem cells (MSCs) is still poorly understood in detail.

Methods

Human MSCs were isolated and cultivated onto the surface of a 3 × 3 mm porcine collagen I/III carrier. After incubation, cell cultures were transfered to the different cutures systems: regular static tissue flasks (group I), spinner flasks (group II) and rotating wall vessels (group III). Following standard protocols cells were stimulated lineage specific towards the osteogenic and chondrogenic lines. To evaluate the effects of applied cytomechanical forces towards cellular differentiation distinct parameters were measured (morphology, antigen and antigen expression) after a total cultivation period of 21 days in vitro.

Results

Depending on the cultivation technique we found significant differences in both gen and protein expression.

Conclusion

Cytomechanical forces with rotational components strongly influence the osteogenic and chondrogenic differentiation.

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Titel: Spinning around or stagnation - what do osteoblasts and chondroblasts really like?
verfasst von: C Zilkens
T Lögters
B Bittersohl
R Krauspe
S Lensing-Höhn
M Jäger
Publikationsdatum: 01.12.2010
Verlag: BioMed Central
Erschienen in: European Journal of Medical Research / Ausgabe 1/2010
Elektronische ISSN: 2047-783X
DOI: https://doi.org/10.1186/2047-783X-15-1-35

Springer Medizin

Abstract

Objective

Methods

Results

Conclusion

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