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Odontology

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01.02.2019 | Original Article

Structural and biomechanical changes to dentin extracellular matrix following chemical removal of proteoglycans

verfasst von: Ana Paula Farina, Cristina M. P. Vidal, Doglas Cecchin, Thaiane R. Aguiar, Ana K. Bedran-Russo

Erschienen in: Odontology | Ausgabe 3/2019

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Abstract

Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly to type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induces pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.

Bertassoni LE, Swain MV. The contribution of proteoglycans to the mechanical behavior of mineralized tissues. J Mech Behav Biomed Mater. 2014;38:91–104.CrossRefPubMed

Imbeni V, Kruzicm JJ, Marshall GW, Marshall SJ, Ritchie RO. The dentin-enamel junction and the fracture of human teeth. Nat Mater. 2005;4:229–32.CrossRefPubMed

Bertassoni LE, Orgel JP, Antipova O, Swain MV. The dentin organic matrix—limitations of restorative dentistry hidden on the nanometer scale. Acta Biomater. 2012;8:2419–33.CrossRefPubMedPubMedCentral

Dechichi P, Biffi JC, Moura CC, de Ameida AW. A model of the early mineralization process of mantle dentin. Micron. 2007;38:486–91.CrossRefPubMed

Goldberg M, Ono M, Septier D, Bonnefoix M, Kilts TM, Bi Y, et al. Fibromodulin-deficient mice reveal dual functions for fibromodulin in regulating dental tissue and alveolar bone formation. Cells Tissues Organs. 2009;189:198–202.CrossRefPubMed

Waddington RJ, Hall RC, Embery G, Lloyd DM. Changing profiles of proteoglycans in the transition of predentine to dentine. Matrix Biol. 2003;22:153–61.CrossRefPubMed

Ho SP, Sulyanto RM, Marshall SJ, Marshall GW. The cementum-dentin junction also contains glycosaminoglycans and collagen fibrils. J Struct Biol. 2005;151:69–78.CrossRefPubMed

Bourdon MA, Oldberg A, Pierschbacher M, Ruoslahti E. Molecular cloning and sequence analysis of a chondroitin sulfate proteoglycan cDNA. Proc Natl Acad Sci. 1985;82:1321–5.CrossRefPubMedPubMedCentral

Scott JE. Alcian blue. Now you see it, now you don’t. Eur J Oral Sci. 1996;104:2–9.CrossRefPubMed

10.

Vogel KG, Paulsson M, Heinegård D. Specific inhibition of type I and type II collagen fibrillogenesis by the small proteoglycan of tendon. Biochem J. 1984;223:587–97.CrossRefPubMedPubMedCentral

11.

Hedbom E, Heinegård D. Binding of fibromodulin and decorin to separate sites on fibrillar collagens. J Biol Chem. 1993;268:27307–12.PubMed

12.

Kobe B, Deisenhofer J. The leucine-rich repeat: a versatile binding motif. Trends Biochem Sci. 1994;19:415–21.CrossRefPubMed

13.

Schönherr E, Witsch-Prehm P, Harrach B, Robenek H, Rauterberg J, Kresse H. Interaction of biglycan with type I collagen. J Biol Chem. 1995;270:2776–83.CrossRefPubMed

14.

Septier D, Hall RC, Lloyd D, Embery G, Goldberg M. Quantitative immunohistochemical evidence of a functional gradient of chondroitin 4-sulphate/dermatan sulphate, developmentally regulated in the predentine of rat incisor. Histochem J. 1998;30:275–84.CrossRefPubMed

15.

Iozzo RV. Matrix proteoglycans: from molecular design to cellular function. Annu Rev Biochem. 1998;67:609–52.CrossRefPubMed

16.

Goldberg M, Septier D, Oldberg A, Young MF, Ameye LG. Fibromodulin-deficient mice display impaired collagen fibrillogenesis in predentin as well as altered dentin mineralization and enamel formation. J Histochem Cytochem. 2006;54:525–37.CrossRefPubMed

17.

Bedran-Russo AK, Pashley DH, Agee K, Drummond JL, Miescke KJ. Changes in stiffness of demineralized dentin following application of collagen crosslinkers. J Biomed Mater Res B Appl Biomater. 2008;86:330–34.CrossRefPubMed

18.

Mazzoni A, Pashley DH, Ruggeri A Jr, Vita F, Falconi M, Di Lenarda R, Breschi L. Adhesion to chondroitinase ABC treated dentin. J Biomed Mater Res B Appl Biomater. 2008;86:228–36.CrossRefPubMed

19.

Chandrasekhar S, Esterman MA, Hoffman HA. Microdetermination of proteoglycans and glycosaminoglycans in the presence of guanidine hydrochloride. Anal Biochem. 1987;161:103–08.CrossRefPubMed

20.

Barbosa I, Garcia S, Barbier-Chassefière V, Caruelle JP, Martelly I, Papy-García D. Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies. Glycobiology. 2003;13:647–53.CrossRefPubMed

21.

Yang Y, Rupani A, Bagnaninchi P, Wimpenny I, Weightman A. Study of optical properties and proteoglycan content of tendons by polarization sensitive optical coherence tomography. J Biomed Opt. 2012;17:081417.CrossRefPubMed

22.

Bedran-Russo AK, Castellan CS, Shinohara MS, Hassan L, Antunes A. Characterization of biomodified dentin matrices for potential preventive and reparative therapies. Acta Biomater. 2011;7:1735–41.CrossRefPubMed

23.

Bedran-Russo AK, Pereira PN, Duarte WR, Okuyama K, Yamauchi M. Removal of dentin matrix proteoglycans by trypsin digestion and its effect on dentin bonding. J Biomed Mater Res B Appl Biomater. 2008;85:261–6.CrossRefPubMed

24.

Castellan CS, Pereira PN, Viana G, Chen SN, Pauli GF, Bedran-Russo AK. Solubility study of phytochemical cross-linking agents on dentin stiffness. J Dent. 2010;38:431–6.CrossRefPubMedPubMedCentral

25.

Reddy GK, Enwemeka CS. A simplified method for the analysis of hydroxyproline in biological tissues. Clin Biochem. 1996;29:225–9.CrossRefPubMed

26.

Vogel KG, Peters JA. Histochemistry defines a proteoglycan-rich layer in bovine flexor tendon subjected to bending. J Musculoskelet Neuronal Interact. 2005;5:64–9.PubMed

27.

Mazzocca AD, McCarthy MB, Ledgard FA, Chowaniec DM, McKinnon WJ Jr, Delaronde S, et al. Histomorphologic changes of the long head of the biceps tendon in common shoulder pathologies. Arthroscopy. 2013;29:972–81.CrossRefPubMed

28.

Fisher MC, Li Y, Seghatoleslami MR, Dealy CN, Kosher RA. Heparan sulfate proteoglycans including syndecan-3 modulate BMP activity during limb cartilage differentiation. Matrix Biol. 2006;25:27–39.CrossRefPubMed

29.

Scott JE. Proteoglycan histochemistry a valuable tool for connective tissue biochemists. Coll Relat Res. 1985;5:541–75.CrossRefPubMed

30.

Rabau MY, Dayan D. Polarization microscopy of picrosirius red stained sections: a useful method for qualitative evaluation of intestinal wall collagen. Histol Histopathol. 1994;9:525–8.PubMed

31.

Yamauchi N, Nagaoka H, Yamauchi S, Teixeira FB, Miguez P, Yamauchi M. Immunohistological characterization of newly formed tissues after regenerative procedure in immature dog teeth. J Endod. 2011;37:1636–41.CrossRefPubMed

32.

Ribeiro JF, dos Anjos EH, Mello ML, de Campos Vidal B. Skin collagen fiber molecular order: a pattern of distributional fiber orientation as assessed by optical anisotropy and image analysis. PLoS One. 2013;8:e54724.CrossRefPubMedPubMedCentral

33.

Dolber PC, Spach MS. Picrosirius red staining of cardiac muscle following phosphomolybdic acid treatment. Stain Technol. 1987;62:23–6.CrossRefPubMed

34.

Diaz Encarnacion MM, Griffin MD, Slezak JM, Bergstralh EJ, Stegall MD, Velosa JA, et al. Correlation of quantitative digital image analysis with the glomerular filtration rate in chronic allograft nephropathy. Am J Transplant. 2004;4:248–56.CrossRefPubMed

35.

Lattouf R, Younes R, Lutomski D, Naaman N, Godeau G, Senni K, et al. Picrosirius red staining: a useful tool to appraise collagen networks in normal and pathological tissues. J Histochem Cytochem. 2014;62:751–8.CrossRefPubMed

36.

Provenzano PP, Vanderby R Jr. Collagen fibril morphology and organization: implications for force transmission in ligament and tendon. Matrix Biol. 2006;25:71–84.CrossRefPubMed

37.

Zhang G, Ezura Y, Chervoneva I, Robinson PS, Beason DP, Carine ET, et al. Decorin regulates assembly of collagen fibrils and acquisition of biomechanical properties during tendon development. J Cell Biochem. 2006;98:1436–49.CrossRefPubMed

38.

Bertassoni LE, Stankoska K, Swain MV. Insights into the structure and composition of the peritubular dentin organic matrix and the lamina limitans. Micron. 2012;43:229–36.CrossRefPubMed

39.

Scott JE. Proteoglycan–fibrillar collagen interactions. Biochem J. 1988;252:313–23.CrossRefPubMedPubMedCentral

40.

Breschi L, Gobbi P, Lopes M, Prati C, Falconi M, Teti G, Mazzotti G. Immunocytochemical analysis of dentin: a double-labeling technique. J Biomed Mater Res A. 2003;67:11–7.CrossRefPubMed

41.

Hsueh MF, Khabut A, Kjellström S, Önnerfjord P, Kraus VB. Elucidating the molecular composition of cartilage by proteomics. J Proteome Res. 2016;15:374–88.CrossRefPubMedPubMedCentral

42.

Goldberg M, Takagi M. Dentine proteoglycans: composition, ultrastructure and functions. Histochem J. 1993;25:781–806.CrossRefPubMed

Titel: Structural and biomechanical changes to dentin extracellular matrix following chemical removal of proteoglycans
verfasst von: Ana Paula Farina
Cristina M. P. Vidal
Doglas Cecchin
Thaiane R. Aguiar
Ana K. Bedran-Russo
Publikationsdatum: 01.02.2019
Verlag: Springer Japan
Erschienen in: Odontology / Ausgabe 3/2019
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-018-00408-0

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Structural and biomechanical changes to dentin extracellular matrix following chemical removal of proteoglycans

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