The online version of this article (https://doi.org/10.1186/s12879-018-2965-4) contains supplementary material, which is available to authorized users.
Zika virus (ZIKV) has been identified in several body fluids of infected individuals. In most cases, it remained detected in blood from few days to 1 week after the onset of symptoms, and can persist longer in urine and in semen. ZIKV infection can have dramatic consequences such as microcephaly and Guillain-Barré syndrome. ZIKV sexual transmission has been documented. A better understanding of ZIKV presence and persistence across biologic compartments is needed to devise rational measures to prevent its transmission.
This observational cohort study will recruit non-pregnant participants aged 18 years and above with confirmed ZIKV infection [positive reverse transcriptase-polymerase chain reaction (RT-PCR) test in blood and/or urine]: symptomatic men and women in ZIKV infection acute phase, and their symptomatic or asymptomatic household/sexual infected contacts. Specimens of blood, urine, semen, vaginal secretion/menstrual blood, rectal swab, oral fluids, tears, sweat, urine and breast milk (if applicable) will be collected at pre-established intervals and tested for ZIKV RNA presence by RT-PCR, other co-infection (dengue, Chikungunya, HIV, hepatitis B and C, syphilis), antibody response (including immunoglobulins M and G), plaque reduction neutralization test (if simultaneously positive for ZIKV and dengue), and ZIKV culture and RNA sequencing. Data on socio-demographic characteristics and comorbidities will be collected in parallel. Participants will be followed up for 12 months.
This prolonged longitudinal follow-up of ZIKV infected persons with regular biologic testing and data collection will offer a unique opportunity to investigate the presence and persistence of ZIKV in various biologic compartments, their clinical and immunological correlates as well as the possibility of ZIKV reactivation/reinfection over time. This valuable information will substantially contribute to the body of knowledge on ZIKV infection and serve as a base for the development of more effective recommendation on the prevention of ZIKV transmission.
NCT03106714. Registration Date: April, 7, 2017
Additional file 1: Questionnaire_screening. (PDF 370 kb)12879_2018_2965_MOESM1_ESM.pdf
Additional file 2: Questionnaire_enrolment_female. (PDF 460 kb)12879_2018_2965_MOESM2_ESM.pdf
Additional file 3: Questionnaire_enrolment_male. (PDF 444 kb)12879_2018_2965_MOESM3_ESM.pdf
Additional file 4: Questionnaire_follow.up_female. (PDF 311 kb)12879_2018_2965_MOESM4_ESM.pdf
Additional file 5: Questionnaire_follow.up_male. (PDF 299 kb)12879_2018_2965_MOESM5_ESM.pdf
Additional file 6: Consultation_form_1st_visit. (PDF 608 kb)12879_2018_2965_MOESM6_ESM.pdf
Additional file 7: Consultation_form_2st_visit. (PDF 350 kb)12879_2018_2965_MOESM7_ESM.pdf
WHO Emergencies. Zika situation report. 2 June 2016. http://www.who.int/emergencies/zika-virus/situation-report/2-june-2016/en/. Accessed 8 Jun 2016.
PAHO Case Definitions. http://www.paho.org/hq/index.php?option=com_content&view=article&id=11117&Itemid=41532&lang=en. Accessed 26 Apr 2016.
Brasil P, Calvet GA, Siqueira AM, Wakimoto M, de Sequeira PC, Nobre A, Quintana Mde S, Mendonca MC, Lupi O, de Souza RV, et al. Zika virus outbreak in Rio de Janeiro, Brazil: clinical characterization, epidemiological and Virological aspects. PLoS Negl Trop Dis. 2016;10(4):e0004636. CrossRefPubMedPubMedCentral
Rapid risk assessment: Zika virus epidemic in the Americas: potential association with microcephaly and Guillain-Barré syndrome. Stockholm: European Centre for Disease Prevention and Control, December 10, 2015. http://ecdc.europa.eu/en/publications/Publications/zika-virus-americas-association-with-microcephaly-rapid-risk-assessment.pdf. Accessed 8 Jun 2016.
Pan American Health Organization/World Health Organization. Neurological syndrome, congenital malformations and Zika virus infection. Implications for public health in the Americas. Epidemiological Alert 2015. http://www.paho.org/hq/index.php?option=com_docman&task=doc_view&Itemid=270&gid=32879&lang=en. Accessed 8 Jun 2016.
WHO Media Center. WHO Director-General summarizes the outcome of the Emergency Committee regarding clusters of microcephaly and Guillain-Barré syndrome. 01/02/2016. http://www.who.int/mediacentre/news/statements/2016/emergency-committee-zika-microcephaly/en/ Accessed 12 May 2016.
World Health Organization. Microcephaly Fact sheet. http://www.who.int/mediacentre/factsheets/microcephaly/en/. Accessed 4 May 2016.
Hills SL, Russell K, Hennessey M, et al. Transmission of Zika Virus Through Sexual Contact with Travelers to Areas of Ongoing Transmission — Continental United States, 2016. MMWR Morb Mortal Wkly Rep. 2016;65:215–216. http://dx.doi.org/10.15585/mmwr.mm6508e2. Accessed 19 Jan 2018.
Calisher CH, Karabatsos N, Dalrymple JM, Shope RE, Porterfield JS, Westaway EG, Brandt WE: Antigenic relationships between flaviviruses as determined by cross-neutralization tests with polyclonal antisera. J. Gen. Virol 1989, 70 ( Pt 1):37-43.
Freour T, Mirallie S, Hubert B, Splingart C, Barriere P, Maquart M, Leparc-Goffart I. Sexual transmission of Zika virus in an entirely asymptomatic couple returning from a Zika epidemic area, France, April 2016. Euro Surveill. 2016;21(23). https://doi.org/10.2807/1560-7917.ES.2016.21.23.30254.
From the Centers for Disease Control and Prevention. Possible West Nile virus transmission to an infant through breast-feeding--Michigan, 2002. JAMA. 2002;288(16):1976–7.
WHO Interim Guidance. Breastfeeding in the context of Zika virus. 2016. http://apps.who.int/iris/bitstream/10665/204473/1/WHO_ZIKV_MOC_16.5_eng.pdf?ua=1. Accessed 8 May 2016.
Prefeitura do Rio de Janeiro. Clinicas de familia: a nova rede de atendimento da cidade. http://www.rio.rj.gov.br/web/sms/clinicas-da-familia. Accessed 8 May 2016.
Portal Brasil. http://www.brasil.gov.br/saude/2011/08/unidades-ajudam-a-desafogar-os-prontos-socorros. Accessed 8 May 2016.
- Study on the persistence of Zika virus (ZIKV) in body fluids of patients with ZIKV infection in Brazil
Guilherme Amaral Calvet
Edna Oliveira Kara
Silvana Pereira Giozza
Camila Helena Aguiar Bôtto-Menezes
Rafael Freitas de Oliveira Franca
Marcus Vinicius Guimarães de Lacerda
Marcia da Costa Castilho
Patrícia Carvalho de Sequeira
Maeve Brito de Mello
Ximena Pamela Diaz Bermudez
Adele Schwartz Benzaken
Ana Maria Bispo de Filippis
Nathalie Jeanne Nicole Broutet
for the ZIKABRA Study Team
- BioMed Central
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