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Journal of Cancer Research and Clinical Oncology

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01.01.2009 | Original Paper

Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma

verfasst von: Salvatore De Maria, Giuseppe Pannone, Pantaleo Bufo, Angela Santoro, Rosario Serpico, Salvatore Metafora, Corrado Rubini, Daniela Pasquali, Silvana M. Papagerakis, Stefania Staibano, Gaetano De Rosa, Ernesto Farina, Monica Emanuelli, Andrea Santarelli, Maria Ada Mariggiò, Lucio Lo Russo, Lorenzo Lo Muzio

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 1/2009

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Abstract

Purpose

Survivin, an inhibitor of apoptosis protein and a cell cycle regulator, has been detected in the majority of human cancers. Five splice variants (survivin, survivin-2α, survivin-2B, survivin-3B, and survivin-ΔEx3) have been identified; their expressions have been investigated here.

Methods

By means of RT real-time PCR and immunohistochemistry, we have evaluated survivin isoform expressions at both mRNA and protein levels in human normal oral tissue, precancerous lesions, and oral squamous cell carcinoma (OSCC). Their correlations with the pathological findings have also been analyzed.

Results

Expression levels of all survivin transcript variants were markedly elevated in OSCC when compared to normal tissues. One-way analysis of variance (ANOVA) revealed highly significant up-regulation of survivin (P = 0.001), survivin-ΔEx3 (P = 0.001) and survivin-2B (P = 0.004), whereas survivin-3B showed a minor increase in OSCC compared to normal mucosa.

Conclusions

Our findings suggest that survivin isoforms deregulation may have significant implications in tumor aggressiveness and prognosis.

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Titel: Survivin gene-expression and splicing isoforms in oral squamous cell carcinoma
verfasst von: Salvatore De Maria
Giuseppe Pannone
Pantaleo Bufo
Angela Santoro
Rosario Serpico
Salvatore Metafora
Corrado Rubini
Daniela Pasquali
Silvana M. Papagerakis
Stefania Staibano
Gaetano De Rosa
Ernesto Farina
Monica Emanuelli
Andrea Santarelli
Maria Ada Mariggiò
Lucio Lo Russo
Lorenzo Lo Muzio
Publikationsdatum: 01.01.2009
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 1/2009
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0433-z

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