nach oben

Lasers in Medical Science

Erschienen in:

02.06.2022 | Original Article

The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study

verfasst von: Pornnutchar Sethasathien, Pornpen Tantivitayakul, Thitiwan Teparat-Burana

Erschienen in: Lasers in Medical Science | Ausgabe 6/2022

Einloggen, um Zugang zu erhalten

Abstract

In vitro bacterial elimination using the erbium, chromium: yttrium–scandium–gallium–garnet (Er,Cr:YSGG) laser against periodontopathic bacteria was investigated. Bacterial suspensions of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis were spread on agar plates and the Er,Cr:YSGG laser was applied at 40 mJ pulse energy for durations of 30 s, 60 s, and 90 s. The agar plates were incubated, and growth inhibition zones were assessed. Optimal laser irradiation durations to achieve maximal bacterial elimination were evaluated using laser ablation on the bacterial colonies. The remaining viable bacteria were determined by the colony-forming unit (CFU) counting method. Growth inhibition zones were observed at all irradiation durations for both A. actinomycetemcomitans and P. gingivalis. Mean logarithmic values of CFU/ml after bacterial colony irradiation for 0 s (control), 12 s × 1 lap, 24 s × 1 lap, 48 s × 1 lap, and 24 s × 2 laps were 8.82 ± 0.35, 7.31 ± 0.94, 6.32 ± 0.61, 3.17 ± 2.90, and 0.00, respectively, for A. actinomycetemcomitans and 9.83 ± 0.50, 9.42 ± 0.11, 6.90 ± 1.60, 2.33 ± 3.19, and 0.00 for P. gingivalis. Significant differences were found between the control group and the two irradiated groups 48 s × 1 lap and 24 s × 2 laps (p < 0.05), and also between irradiated groups 12 s × 1 lap and 24 s × 2 laps (p < 0.05). An Er,Cr:YSGG laser with power setting 1.5 W and 30 Hz frequency showed potential for bacterial elimination against A. actinomycetemcomitans and P. gingivalis in vitro. Significant bacterial elimination (> 99.99%) was observed after 48 s of irradiation.

Papapanou PN, Sanz M, Buduneli N, Dietrich T, Feres M, Fine DH, Flemmig TF, Garcia R, Giannobile WV, Graziani F (2018) Periodontitis: consensus report of workgroup 2 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. J Periodontol 89:S173–S182. https://doi.org/10.1002/JPER.17-0721CrossRefPubMed

Socransky S, Haffajee A, Cugini M, Smith C, Kent R (1998) Microbial complexes in subgingival plaque. J Clin Periodontol 25(2):134–144. https://doi.org/10.1111/j.1600-051x.1998.tb02419.xCrossRefPubMed

Wara-Aswapati N, Pitiphat W, Chanchaimongkon L, Taweechaisupapong S, Boch J, Ishikawa I (2009) Red bacterial complex is associated with the severity of chronic periodontitis in a Thai population. Oral Dis 15(5):354–359. https://doi.org/10.1111/j.1601-0825.2009.01562.xCrossRefPubMed

Torrungruang K, Bandhaya P, Likittanasombat K, Grittayaphong C (2009) Relationship between the presence of certain bacterial pathogens and periodontal status of urban Thai adults. J Periodontol 80(1):122–129. https://doi.org/10.1902/jop.2009.080248CrossRefPubMed

Dzink J, Tanner A, Haffajee A, Socransky S (1985) Gram negative species associated with active destructive periodontal lesions. J Clin Periodontol 12(8):648–659. https://doi.org/10.1111/j.1600-051x.1985.tb00936.xCrossRefPubMed

Slots J, Ting M (1999) Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment. Periodontol 2000 20(1):82–121. https://doi.org/10.1111/j.1600-0757.1999.tb00159.xCrossRefPubMed

Bollen CM, Mongardini C, Papaioannou W, Van Steenberghe D, Quirynen M (1998) The effect of a one-stage full-mouth disinfection on different intra-oral niches clinical and microbiological observations. J Clin Periodontol 25(1):56–66. https://doi.org/10.1111/j.1600-051x.1998.tb02364.xCrossRefPubMed

Kepic TJ, O’Leary TJ, Kafrawy AH (1990) Total calculus removal: an attainable objective? J Periodontol 61(1):16–20. https://doi.org/10.1902/jop.1990.61.1.16CrossRefPubMed

Waerhaug J (1978) Healing of the dento-epithelial junction following subgingival plaque control: ii: as observed on extracted teeth. J Periodontol 49(3):119–134. https://doi.org/10.1902/jop.1978.49.3.119CrossRefPubMed

10.

Fleischer HC, Mellonig JT, Brayer WK, Gray JL, Barnett JD (1989) Scaling and root planing efficacy in multirooted teeth. J Periodontol 60(7):402–409. https://doi.org/10.1902/jop.1989.60.7.402CrossRefPubMed

11.

Adriaens PA, Edwards CA, De Boever JA, Loesche WJ (1988) Ultrastructural observations on bacterial invasion in cementum and radicular dentin of periodontally diseased human teeth. J Periodontol 59(8):493–503. https://doi.org/10.1902/jop.1988.59.8.493CrossRefPubMed

12.

Sbordone L, Ramaglia L, Gulletta E, Iacono V (1990) Recolonization of the subgingival microflora after scaling and root planing in human periodontitis. J Periodontol 61(9):579–584. https://doi.org/10.1902/jop.1990.61.9.579CrossRefPubMed

13.

Magnusson I, Lindhe J, Yoneyama T, Liljenberg B (1984) Recolonization of a subgingival microbiota following scaling in deep pockets. J Clin Periodontol 11(3):193–207. https://doi.org/10.1111/j.1600-051x.1984.tb01323.xCrossRefPubMed

14.

Renvert S, Wikström M, Dahlén G, Slots J, Egelberg J (1990) Effect of root debridement on the elimination of Actinobacillus actinomycetemcomitans and Bacteroides gingivalis from periodontal pockets. J Clin Periodontol 17(6):345–350. https://doi.org/10.1111/j.1600-051x.1990.tb00029.xCrossRefPubMed

15.

Loesche W, Syed S, Schmidt E, Morrison E (1985) Bacterial profiles of subgingival plaques in periodontitis. J Periodontol 56(8):447–456. https://doi.org/10.1902/jop.1985.56.8.447CrossRefPubMed

16.

Drisko CH (1996) Non-surgical pocket therapy: pharmacotherapeutics. Ann Periodontol 1(1):491–566. https://doi.org/10.1902/annals.1996.1.1.491CrossRefPubMed

17.

Drisko CH (2001) Nonsurgical periodontal therapy. Periodontol 2000 25(1):77–88. https://doi.org/10.1034/j.1600-0757.2001.22250106.xCrossRefPubMed

18.

Berezow AB, Darveau RP (2011) Microbial shift and periodontitis. Periodontol 2000 55(1):36–47. https://doi.org/10.1111/j.1600-0757.2010.00350.xCrossRefPubMedPubMedCentral

19.

Slots J, Ting M (2002) Ting M (2002) Systemic antibiotics in the treatment of periodontal disease. Periodontol 2000 28(1):106–176. https://doi.org/10.1034/j.1600-0757.2002.280106.xCrossRefPubMed

20.

Aoki A, Sasaki KM, Watanabe H, Ishikawa I (2004) Lasers in nonsurgical periodontal therapy. Periodontol 2000 36(1):59–97. https://doi.org/10.1111/j.1600-0757.2004.03679.xCrossRefPubMed

21.

Coluzzi DJ (2004) Fundamentals of dental lasers: science and instruments. Dental Clinics 48(4):751–770. https://doi.org/10.1016/j.cden.2004.05.003CrossRefPubMed

22.

Ando Y, Aoki A, Watanabe H, Ishikawa I (1996) Bactericidal effect of erbium YAG laser on periodontopathic bacteria. Lasers Surg Med: The Official Journal of the American Society for Laser Medicine and Surgery 19(2):190–200. https://doi.org/10.1002/(SICI)1097-0266(199903)20:3%3c205::AID-SMJ17%3e3.0.CO;2-HCrossRef

23.

Akiyama F, Aoki A, Miura-Uchiyama M, Sasaki KM, Ichinose S, Umeda M, Ishikawa I, Izumi Y (2011) In vitro studies of the ablation mechanism of periodontopathic bacteria and decontamination effect on periodontally diseased root surfaces by erbium: yttrium–aluminum–garnet laser. Lasers Med Sci 26(2):193–204. https://doi.org/10.1007/s10103-010-0763-3CrossRefPubMed

24.

Folwaczny M, Mehl A, Aggstaller H, Hickel R (2002) Antimicrobial effects of 2.94 μm Er: YAG laser radiation on root surfaces: an in vitro study. J Clin Periodontol 29(1):73–78. https://doi.org/10.1034/j.1600-051x.2002.290111.xCrossRefPubMed

25.

Tomasi C, Schander K, Dahlén G, Wennström JL (2006) Short-term clinical and microbiologic effects of pocket debridement with an Er: YAG laser during periodontal maintenance. J Periodontol 77(1):111–118. https://doi.org/10.1902/jop.2006.77.1.111CrossRefPubMed

26.

Zhou X, Lin M, Zhang D, Song Y, Wang Z (2019) Efficacy of Er:YAG laser on periodontitis as an adjunctive non-surgical treatment: a split-mouth randomized controlled study. J Clin Periodontol 46(5):539–547. https://doi.org/10.1111/jcpe.13107CrossRefPubMed

27.

Zengin Celik T, Saglam E, Ercan C, Akbas F, Nazaroglu K, Tunali M (2019) Clinical and microbiological effects of the use of erbium: yttrium-aluminum-garnet laser on chronic periodontitis in addition to nonsurgical periodontal treatment: a randomized clinical trial-6 months follow-up. Photobiomodul Photomed Laser Surg 37(3):182–190. https://doi.org/10.1089/photob.2018.4510CrossRefPubMed

28.

Sgolastra F, Petrucci A, Gatto R, Monaco A (2012) Efficacy of Er: YAG laser in the treatment of chronic periodontitis: systematic review and meta-analysis. Lasers Med Sci 27(3):661–673. https://doi.org/10.1007/s10103-011-0928-8CrossRefPubMed

29.

Joseph S (1997) The physics of surgical laser. Oral Maxillofacial Surg Clin North Am 9(1):33–47CrossRef

30.

Magaz VR, Alemany AS, Alfaro FH, Molina JN (2016) Efficacy of Adjunctive Er, Cr:YSGG laser application following scaling and root planing in periodontally diseased patients. Int J Periodontics Restorative Dent 36(5):715–721. https://doi.org/10.11607/prd.2660

31.

Üstün K, Hatipoğlu M, Daltaban Ö, Felek R, Firat M (2018) Clinical and biochemical effects of erbium, chromium: yttrium, scandium, gallium, garnet laser treatment as a complement to periodontal treatment. Niger J Clin Pract 21(9):1150–1157PubMed

32.

Kim H-W, Lee W-P, Yu S-J, Kim B-O (2017) Non-surgical periodontal treatment using the erbium, chromium: yttrium–scandium–gallium–garnet (Er, Cr: YSGG) laser in chronic periodontitis. Oral Biol Res 41(3):173–179. https://doi.org/10.21851/obr.41.03.201709.173CrossRef

33.

Sezen D, Hatipoğlu M, Üstün K (2020) Evaluation of the clinical and biochemical efficacy of erbium, chromium: ytrium-scandium-gallium-garnet (ER, CR: YSGG) laser treatment in periodontitis. Lasers Med Sci 35(7):1567–1575. https://doi.org/10.1007/s10103-020-02990-8CrossRefPubMed

34.

Li M-M, Jia J-H, Wu M-X, Zhao C-Y, Jia L-Y, Shi H, Zhang X-L (2020) Clinical effectiveness of Er, Cr: YSGG lasers in non-surgical treatment of chronic periodontitis: a meta-analysis of randomized controlled trials. Lasers Med Sci 36(4):1–13. https://doi.org/10.1007/s10103-020-03156-2CrossRef

35.

Dyer B, Sung EC (2012) Minimally invasive periodontal treatment using the Er, Cr: YSGG laser. A 2-year retrospective preliminary clinical study. The open dentistry journal 6:74. https://doi.org/10.2174/1874210601206010074CrossRefPubMedPubMedCentral

36.

Gupta M, Lamba A, Verma M, Faraz F, Tandon S, Chawla K, Koli D (2013) Comparison of periodontal open flap debridement versus closed debridement with Er, Cr: YSGG laser. Aust Dent J 58(1):41–49. https://doi.org/10.1111/adj.12021CrossRefPubMed

37.

Clem D, Heard R, McGuire M, Scheyer ET, Richardson C, Toback G, Gwaltney C, Gunsolley JC (2020) Comparison of Er, Cr: YSGG laser to minimally invasive surgical technique in the treatment of intrabony defects: six-month results of a multicenter, randomized, controlled study. J Periodontol 92(4):496–506. https://doi.org/10.1002/JPER.20-0028CrossRefPubMed

38.

Gutknecht N, Van Betteray C, Ozturan S, Vanweersch L, Franzen R (2015) Laser supported reduction of specific microorganisms in the periodontal pocket with the aid of an Er, Cr: YSGG laser: a pilot study. Sci World J 2015:450258. https://doi.org/10.1155/2015/450258CrossRef

39.

Strever JM, Lee J, Ealick W, Peacock M, Shelby D, Susin C, Mettenberg D, El-Awady A, Rueggeberg F, Cutler CW (2017) Erbium, chromium: yttrium-scandium-gallium-garnet laser effectively ablates single-species biofilms on titanium disks without detectable surface damage. J Periodontol 88(5):484–492. https://doi.org/10.1902/jop.2016.160529CrossRefPubMed

40.

Biolase (2021) Effectively manage periodontitis with repair. brochure, Foothill Ranch, California, USA. https://www.biolase.com/media/21-2052-REPAIRProtocolGuide_r1dig.pdf. Accessed 15 Apr 2022

41.

Ge L, Zhang Y, Shu R (2017) Er, Cr: YSGG laser application for the treatment of periodontal furcation involvements. Photomed Laser Surg 35(2):92–97. https://doi.org/10.1089/pho.2016.4145CrossRefPubMed

42.

Dereci Ö, Hatipoğlu M, Sindel A, Tozoğlu S, Üstün K (2016) The efficacy of Er, Cr: YSGG laser supported periodontal therapy on the reduction of peridodontal disease related oral malodor: a randomized clinical study. Head Face Med 12(1):1–7. https://doi.org/10.1186/s13005-016-0116-yCrossRef

43.

Kelbauskiene S, Baseviciene N, Goharkhay K, Moritz A, Machiulskiene V (2011) One-year clinical results of Er, Cr: YSGG laser application in addition to scaling and root planing in patients with early to moderate periodontitis. Lasers Med Sci 26(4):445–452. https://doi.org/10.1007/s10103-010-0799-4CrossRefPubMed

44.

Wilson C, Lukowicz R, Merchant S, Valquier-Flynn H, Caballero J, Sandoval J, Okuom M, Huber C, Brooks TD, Wilson E, Clement B, Wentworth CD, Holmes AE (2017) Quantitative and qualitative assessment methods for biofilm growth: A Mini-review. Res Rev J Eng Technol 6(4)

45.

Chen C, Slots J (1999) Microbiological tests for Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. Periodontol 2000 20(1):53–64. https://doi.org/10.1111/j.1600-0757.1999.tb00157.xCrossRefPubMed

Titel: The effect of Er,Cr:YSGG laser on periodontopathic bacteria elimination: an in vitro study
verfasst von: Pornnutchar Sethasathien
Pornpen Tantivitayakul
Thitiwan Teparat-Burana
Publikationsdatum: 02.06.2022
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 6/2022
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03573-5

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2022

A histological evaluation of the mice oral mucosal tissue wounds excised with diode laser, Er:YAG laser, and cold scalpel

Long-term results of micropulse laser trabeculoplasty with 577-nm yellow wavelength in patients with uncontrolled primary open-angle glaucoma and pseudoexfoliation glaucoma

Effects of high-frequency near infrared laser irradiation on experimental tooth movement–induced pain in rats

Laser ablation of pancreatic cancer using a cylindrical light diffuser

Evaluation of Er,Cr:YSGG laser irradiation for debonding of zirconia hybrid abutment crowns from titanium bases

Nitric oxide storage levels modulate vasodilation and the hypotensive effect induced by photobiomodulation using an aluminum gallium arsenide (AlGaAs) diode laser (660 nm)