nach oben

NeuroMolecular Medicine

Erschienen in:

26.03.2021 | Original Paper

The Effect of Ulinastatin to the Learning and Memory in Zebrafish

verfasst von: Ji Won Yoo, Sung-Hwa Sohn, Yeon-Hwa Kim, Too Jae Min

Erschienen in: NeuroMolecular Medicine | Ausgabe 4/2021

Einloggen, um Zugang zu erhalten

Abstract

Previous study indicated that Ulinastatin (UTI) increased glutamine uptake by upregulation of glutamate transporters in astrocytes. These glutamate transporters have important role to improve cognitive function in hippocampus. In this study, we wanted to demonstrate whether UTI could improve learning and memory by using zebrafish behavior model and bio-markers. Zebrafish were 6–8 months of age and were 2.5–3.5 cm long. They were divided into four groups by control, 1X PBS-injected control, UTI 10,000, and 50,000 injected group. All PBS and UTI injected zebrafish were anesthetized by Tricainemethanesulphonate. We measured total time, distance moved, and frequency in each compartment of T-maze. We also measured the expression levels of glutamate transporter levels and cognitive bio-markers such as c-fos, c-jun, BDNF. UTI affected the learning and memory in zebrafish in a dose-dependent manner. In 50,000 unit/kg UTI-treated zebrafish, there were increases of time, distance, and frequency in target compartment. In 50,000 unit/kg UTI-treated zebrafish, there was an increase of time in target compartment. There was no difference among control, PBS-injected, and UTI 10,000 unit/kg-treated groups. EAAT4 glutamate transporter, c-fos and BDNF were significantly increased in 50,000 unit/kg UTI-treated group. UTI-enhanced learning and memory in zebrafish. The expressions of EAAT4 glutamate transporter, c- fos and BDNF in zebrafish were highly correlated may play a role.

Abe, K., & Saito, H. (2001). Possible linkage between glutamate transporter and mitogen-activated protein kinase cascade in cultured rat cortical astrocytes. Journal of Neurochemistry, 76(1), 217–223. https://doi.org/10.1046/j.1471-4159.2001.00062.xCrossRefPubMed

Baranov, D., Bickler, P. E., Crosby, G. J., Culley, D. J., Eckenhoff, M. F., Eckenhoff, R. G., et al. (2009). Consensus statement: First International Workshop on Anesthetics and Alzheimer’s disease. Anesthesia and Analgesia, 108(5), 1627–1630. https://doi.org/10.1213/ane.0b013e318199dc72CrossRefPubMedPubMedCentral

Bekinschtein, P., Cammarota, M., Igaz, L. M., Bevilaqua, L. R., Izquierdo, I., & Medina, J. H. (2007). Persistence of long-term memory storage requires a late protein synthesis- and BDNF- dependent phase in the hippocampus. Neuron, 53(2), 261–277. https://doi.org/10.1016/j.neuron.2006.11.025CrossRefPubMed

Bekinschtein, P., Cammarota, M., & Medina, J. H. (2014). BDNF and memory processing. Neuropharmacology, 76, 677–683. https://doi.org/10.1016/j.neuropharm.2013.04.024CrossRefPubMed

Chatterjee, D., & Gerlai, R. (2009). High precision liquid chromatography analysis of dopaminergic and serotoninergic responses to acute alcohol exposure in zebrafish. Behavioural Brain Research, 200(1), 208–213.CrossRefPubMedPubMedCentral

Chen, C. C., Wang, S. S., & Lee, F. Y. (2007). Action of antiproteases on the inflammatory response in acute pancreatitis. Journal of the Pancreas: JOP, 8(4 Suppl), 488–494.PubMed

Chen, H. M., Huang, H. S., Ruan, L., He, Y. B., & Li, X. J. (2014). Ulinastatin attenuates cerebral ischemia-reperfusion injury in rats. International Journal of Clinical and Experimental Medicine, 7(5), 1483–1489.PubMedPubMedCentral

Danbolt, N. C. (2001). Glutamate uptake. Progress in Neurobiology, 65(1), 1–105.CrossRefPubMed

Dong, X., Zhang, D., Zhang, L., Li, W., & Meng, X. (2012). Osthole improves synaptic plasticity in the hippocampus and cognitive function of Alzheimer’s disease rats via regulating glutamate. Neural Regeneration Research, 7(30), 2325–2332. https://doi.org/10.3969/j.issn.1673-5374.2012.30.001CrossRefPubMedPubMedCentral

Duerson, K., Woltjer, R. L., Mookherjee, P., Leverenz, J. B., Montine, T. J., Bird, T. D., et al. (2009). Detergent-insoluble EAAC1/EAAT3 aberrantly accumulates in hippocampal neurons of Alzheimer’s disease patients. Brain Pathology, 19(2), 267–278. https://doi.org/10.1111/j.1750-3639.2008.00186.xCrossRefPubMed

Gibbs, M. E., Hertz, L., & Ng, K. T. (2004). Inhibition of short-term memory formation in the chick by blockade of extracellular glutamate uptake. Neurobiology of Learning and Memory, 81(2), 115–119. https://doi.org/10.1016/j.nlm.2003.10.002CrossRefPubMed

Gomez, Y., Vargas, J. P., Portavella, M., & Lopez, J. C. (2006). Spatial learning and goldfish telencephalon NMDA receptors. Neurobiology of Learning and Memory, 85(3), 252–262. https://doi.org/10.1016/j.nlm.2005.11.006CrossRefPubMed

Hargreaves, E. L., Cote, D., & Shapiro, M. L. (1997). A dose of MK801 previously shown to impair spatial learning in the radial maze attenuates primed burst potentiation in the dentate gyrus of freely moving rats. Behavioral Neuroscience, 111(1), 35–48. https://doi.org/10.1037//0735-7044.111.1.35CrossRefPubMed

Howe, K., Clark, M. D., Torroja, C. F., Torrance, J., Berthelot, C., Muffato, M., et al. (2013). The zebrafish reference genome sequence and its relationship to the human genome. Nature, 496(7446), 498–503. https://doi.org/10.1038/nature12111CrossRefPubMedPubMedCentral

Inage, Y. W., Itoh, M., Wada, K., & Takashima, S. (1998). Expression of two glutamate transporters, GLAST and EAAT4, in the human cerebellum: their correlation in development and neonatal hypoxic-ischemic damage. Journal of Neuropathology and Experimental Neurology, 57(6), 554–562. https://doi.org/10.1097/00005072-199806000-00003CrossRefPubMed

Inoue, K., & Takano, H. (2010). Urinary trypsin inhibitor as a therapeutic option for endotoxin-related inflammatory disorders. Expert Opinion on Investigational Drugs, 19(4), 513–520. https://doi.org/10.1517/13543781003649533CrossRefPubMed

Karnad, D. R., Bhadade, R., Verma, P. K., Moulick, N. D., Daga, M. K., Chafekar, N. D., et al. (2014). Intravenous administration of ulinastatin (human urinary trypsin inhibitor) in severe sepsis: a multicenter randomized controlled study. Intensive Care Medicine, 40(6), 830–838. https://doi.org/10.1007/s00134-014-3278-8CrossRefPubMedPubMedCentral

Kim, Y.-H., Lee, K. S., Park, A. R., & Min, T. J. (2017). Adding preferred color to a conventional reward method improves the memory of zebrafish in the T-maze behavior model. Animal Cells and Systems, 21(6), 374–381.CrossRef

Kim, Y. S., Won, Y. J., Lim, B. G., Min, T. J., Kim, Y. H., & Lee, I. O. (2020). Neuroprotective effects of magnesium L-threonate in a hypoxic zebrafish model. BMC Neuroscience, 21(1), 29. https://doi.org/10.1186/s12868-020-00580-6CrossRefPubMedPubMedCentral

Kobayashi, H. (2006). Endogenous anti-inflammatory substances, inter-alpha-inhibitor and bikunin. Biological Chemistry, 387(12), 1545–1549. https://doi.org/10.1515/BC.2006.192CrossRefPubMed

Leppa, S., Saffrich, R., Ansorge, W., & Bohmann, D. (1998). Differential regulation of c-Jun by ERK and JNK during PC12 cell differentiation. EMBO Journal, 17(15), 4404–4413. https://doi.org/10.1093/emboj/17.15.4404CrossRef

Levenson, J., Weeber, E., Selcher, J. C., Kategaya, L. S., Sweatt, J. D., & Eskin, A. (2002). Long-term potentiation and contextual fear conditioning increase neuronal glutamate uptake. Nature Neuroscience, 5(2), 155–161. https://doi.org/10.1038/nn791CrossRefPubMed

Li, H. F., Zhao, S. X., Xing, B. P., & Sun, M. L. (2015). Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning. Neural Regeneration Research, 10(3), 467–472. https://doi.org/10.4103/1673-5374.153698CrossRefPubMedPubMedCentral

Lili, X., Zhiyong, H., & Jianjun, S. (2013). A preliminary study of the effects of ulinastatin on early postoperative cognition function in patients undergoing abdominal surgery. Neuroscience Letters, 541, 15–19. https://doi.org/10.1016/j.neulet.2013.02.008CrossRefPubMed

Lopez, J. C., Bingman, V. P., Rodriguez, F., Gomez, Y., & Salas, C. (2000). Dissociation of place and cue learning by telencephalic ablation in goldfish. Behavioral Neuroscience, 114(4), 687–699. https://doi.org/10.1037//0735-7044.114.4.687CrossRefPubMed

Lopez, J. C., Broglio, C., Rodriguez, F., Thinus-Blanc, C., & Salas, C. (2000). Reversal learning deficit in a spatial task but not in a cued one after telencephalic ablation in goldfish. Behavioural Brain Research, 109(1), 91–98. https://doi.org/10.1016/s0166-4328(99)00167-9CrossRefPubMed

Lu, B., Nagappan, G., & Lu, Y. (2014). BDNF and synaptic plasticity, cognitive function, and dysfunction. Handbook of Experimental Pharmacology, 220, 223–250. https://doi.org/10.1007/978-3-642-45106-5_9CrossRefPubMed

Lv, Z. T., Huang, J. M., Zhang, J. M., Zhang, J. M., Guo, J. F., & Chen, A. M. (2016). Effect of Ulinastatin in the Treatment of Postperative Cognitive Dysfunction: Review of Current Literature. BioMed Research International, 2016, 2571080. https://doi.org/10.1155/2016/2571080CrossRefPubMedPubMedCentral

Malinowska, M., Niewiadomska, M., & Wesierska, M. (2016). Spatial memory formation differentially affects c-Fos expression in retrosplenial areas during place avoidance training in rats. Acta Neurobiol Exp (Wars), 76(3), 244–265. https://doi.org/10.21307/ane-2017-024CrossRef

Miglio, G., Varsaldi, F., Dianzani, C., Fantozzi, R., & Lombardi, G. (2005). Stimulation of group I metabotropic glutamate receptors evokes calcium signals and c-jun and c-fos gene expression in human T cells. Biochemical Pharmacology, 70(2), 189–199. https://doi.org/10.1016/j.bcp.2005.04.038CrossRefPubMed

Min, T. J., Kim, W. Y., Kim, Y. S., Yoo, J. W., Lee, H. J., & Lee, I.-O. (2016). Ulinastatin up-regulates glutamate transporters in glial cells. International Journal of Clinical and Experimental Medicine, 9(2), 2367–2374.

Miyashita, T., Kikuchi, E., Horiuchi, J., & Saitoe, M. (2018). Long-Term Memory Engram Cells Are Established by c-Fos/CREB Transcriptional Cycling. Cell Reports, 25(10), 2716-2728.e3. https://doi.org/10.1016/j.celrep.2018.11.022CrossRefPubMed

Moller, J. T., Cluitmans, P., Rasmussen, L. S., Houx, P., Rasmussen, H., Canet, J., et al. (1998). Long-term postoperative cognitive dysfunction in the elderly ISPOCD1 study. ISPOCD investigators. International Study of Post-Operative Cognitive Dysfunction. Lancet, 351(9106), 857–861. https://doi.org/10.1016/s0140-6736(97)07382-0CrossRefPubMed

Monje, P., Hernandez-Losa, J., Lyons, R. J., Castellone, M. D., & Gutkind, J. S. (2005). Regulation of the transcriptional activity of c-Fos by ERK. A novel role for the prolyl isomerase PIN1. Journal of Biological Chemistry, 280(42), 35081–35084. https://doi.org/10.1074/jbc.C500353200CrossRef

Monk, T. G., Weldon, B. C., Garvan, C. W., Dede, D. E., van der Aa, M. T., Heilman, K. M., et al. (2008). Predictors of cognitive dysfunction after major noncardiac surgery. Anesthesiology, 108(1), 18–30. https://doi.org/10.1097/01.anes.0000296071.19434.1eCrossRefPubMed

Newman, M. F., Kirchner, J. L., Phillips-Bute, B., Gaver, V., Grocott, H., Jones, R. H., et al. (2001). Longitudinal assessment of neurocognitive function after coronary-artery bypass surgery. New England Journal of Medicine, 344(6), 395–402. https://doi.org/10.1056/NEJM200102083440601CrossRef

Panja, D., & Bramham, C. R. (2014). BDNF mechanisms in late LTP formation: A synthesis and breakdown. Neuropharmacology, 76, 664–676. https://doi.org/10.1016/j.neuropharm.2013.06.024CrossRefPubMed

Pita-Almenar, J. D., Collado, M. S., Colbert, C. M., & Eskin, A. (2006). Different mechanisms exist for the plasticity of glutamate reuptake during early long-term potentiation (LTP) and late LTP. Journal of Neuroscience, 26(41), 10461–10471. https://doi.org/10.1523/JNEUROSCI.2579-06.2006CrossRefPubMed

Portavella, M., Torres, B., & Salas, C. (2004). Avoidance response in goldfish: emotional and temporal involvement of medial and lateral telencephalic pallium. Journal of Neuroscience, 24(9), 2335–2342. https://doi.org/10.1523/JNEUROSCI.4930-03.2004CrossRefPubMed

Price, C. C., Garvan, C. W., & Monk, T. G. (2008). Type and severity of cognitive decline in older adults after noncardiac surgery. Anesthesiology, 108(1), 8–17. https://doi.org/10.1097/01.anes.0000296072.02527.18CrossRefPubMed

Riedel, G., Platt, B., & Micheau, J. (2003). Glutamate receptor function in learning and memory. Behavioural Brain Research, 140(1–2), 1–47. https://doi.org/10.1016/s0166-4328(02)00272-3CrossRefPubMed

Rio, M. J., & Velez-Pardo, C. (2008). Paraquat induces apoptosis in human lymphocytes: protective and rescue effects of glucose, cannabinoids and insulin-like growth factor-1. Growth Factors, 26(1), 49–60. https://doi.org/10.1080/08977190801984205CrossRefPubMed

Santello, M., & Volterra, A. (2009). Synaptic modulation by astrocytes via Ca2+-dependent glutamate release. Neuroscience, 158(1), 253–259. https://doi.org/10.1016/j.neuroscience.2008.03.039CrossRefPubMed

Shams, S., Rihel, J., Ortiz, J. G., & Gerlai, R. (2017). The zebrafish as a promising tool for modeling human brain disorders: A review based upon an IBNS Symposium. Neuroscience and Biobehavioral Reviews. https://doi.org/10.1016/j.neubiorev.2017.09.002CrossRefPubMed

Shan, X., Zhang, X., Li, X., Xu, G., & Li, Z. (2015). Effect of ulinastatin on postoperative cognitive function in the elderly with fracture. Zhonghua Yi Xue Za Zhi, 95(20), 1586–1589.PubMed

Shu, Y., Yang, Y., Qiu, W., Lu, Z., Li, Y., Bao, J., et al. (2011). Neuroprotection by ulinastatin in experimental autoimmune encephalomyelitis. Neurochemical Research, 36(11), 1969–1977. https://doi.org/10.1007/s11064-011-0520-4CrossRefPubMed

Sommer, C., Gass, P., & Kiessling, M. (1995). Selective c-JUN expression in CA1 neurons of the gerbil hippocampus during and after acquisition of an ischemia-tolerant state. Brain Pathology, 5(2), 135–144. https://doi.org/10.1111/j.1750-3639.1995.tb00587.xCrossRefPubMed

Teather, L. A., Packard, M. G., Smith, D. E., Ellis-Behnke, R. G., & Bazan, N. G. (2005). Differential induction of c-Jun and Fos-like proteins in rat hippocampus and dorsal striatum after training in two water maze tasks. Neurobiology of Learning and Memory, 84(2), 75–84. https://doi.org/10.1016/j.nlm.2005.03.006CrossRefPubMed

von Bohlen Und Halbach, O., & von Bohlen Und Halbach, V. (2018). BDNF effects on dendritic spine morphology and hippocampal function. Cell and Tissue Research, 373(3), 729–741. https://doi.org/10.1007/s00441-017-2782-xCrossRef

Yamada, K., & Nabeshima, T. (2003). Brain-derived neurotrophic factor/TrkB signaling in memory processes. Journal of Pharmacological Sciences, 91(4), 267–270. https://doi.org/10.1254/jphs.91.267CrossRefPubMed

Titel: The Effect of Ulinastatin to the Learning and Memory in Zebrafish
verfasst von: Ji Won Yoo
Sung-Hwa Sohn
Yeon-Hwa Kim
Too Jae Min
Publikationsdatum: 26.03.2021
Verlag: Springer US
Erschienen in: NeuroMolecular Medicine / Ausgabe 4/2021
Print ISSN: 1535-1084
Elektronische ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-021-08653-3

Leitlinien kompakt für die Neurologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Neurologie

Schützt Olivenöl vor dem Tod durch Demenz?

10.05.2024 Morbus Alzheimer Nachrichten

Konsumieren Menschen täglich 7 Gramm Olivenöl, ist ihr Risiko, an einer Demenz zu sterben, um mehr als ein Vierten reduziert – und dies weitgehend unabhängig von ihrer sonstigen Ernährung. Dafür sprechen Auswertungen zweier großer US-Studien.

Bluttest erkennt Parkinson schon zehn Jahre vor der Diagnose

10.05.2024 Parkinson-Krankheit Nachrichten

Ein Bluttest kann abnorm aggregiertes Alpha-Synuclein bei einigen Menschen schon zehn Jahre vor Beginn der motorischen Parkinsonsymptome nachweisen. Mit einem solchen Test lassen sich möglicherweise Prodromalstadien erfassen und die Betroffenen früher behandeln.

Darf man die Behandlung eines Neonazis ablehnen?

08.05.2024 Gesellschaft Nachrichten

In einer Leseranfrage in der Zeitschrift Journal of the American Academy of Dermatology möchte ein anonymer Dermatologe bzw. eine anonyme Dermatologin wissen, ob er oder sie einen Patienten behandeln muss, der eine rassistische Tätowierung trägt.

Wartezeit nicht kürzer, aber Arbeit flexibler

Psychotherapie Medizin aktuell

Fünf Jahren nach der Neugestaltung der Psychotherapie-Richtlinie wurden jetzt die Effekte der vorgenommenen Änderungen ausgewertet. Das Hauptziel der Novellierung war eine kürzere Wartezeit auf Therapieplätze. Dieses Ziel wurde nicht erreicht, es gab jedoch positive Auswirkungen auf andere Bereiche.

Update Neurologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Klimawandel und Gesundheit: Was Sie in der Hausarztpraxis wissen müssen und tun können

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 4/2021

Assessment of Peripheral Blood Lymphocytes in Parents of Autistic Children by Cytokinesis Block Micronucleus Assay

Gene Expression Analysis Identifies Cholesterol Metabolism Dysregulation in Hippocampus of Phenytoin-Resistant Pentylenetetrazol-Kindled Epileptic Mice

Vitamin D3 Supplement Attenuates Blood–Brain Barrier Disruption and Cognitive Impairments in a Rat Model of Traumatic Brain Injury

Correction to: Specific Susceptibility to COVID-19 in Adults with Down Syndrome

Activation of ephrinb1/EPHB2/MAP-2/NMDAR Mediates Hippocampal Neurogenesis Promoted by Transcranial Direct Current Stimulation in Cerebral-Ischemic Mice