The effect of zingiber officinale on prooxidant-antioxidant balance and glycemic control in diabetic patients with ESRD undergoing hemodialysis: a double-blind randomized control trial

End-Stage Renal Disease (ESRD) is a severe form of kidney failure which may require dialysis or a kidney transplant [1]. Various factors may exacerbate renal failure, but diabetes has the most substantial influence [2]. In Iran, the prevalence of ESRD is predicted to rise by around 12% every year, with 48.5 percent of these patients getting hemodialysis [3, 4]. Between 2005 and 2021, the prevalence of diabetes in Iran's population expanded from 7.7% to 14.3%, and 29.2% of the population had prediabetes [2, 3, 5, 6]. This trend implies that the rise in ESRD cases in Iran is linked to the growth of diabetes.

ESRD, without a doubt, necessitates a certain treatment approach, but diabetes is another restriction that requires special care [2, 6]. Poor glycemic management in diabetic ESRD patients was shown to raise renal pressure and inflammation, worsen clinical status and increase mortality risk [1, 2, 6].

Regarding the importance of glycemic control in the patients suffering from diabetes and ESRD undergoing hemodialysis, the constraints in terms of kidney dysfunction, limited medical treatment pathways, and finding an economically effective medical supplement, which could cover both pathways, seems necessary [2, 7‐9]. Among all methods of designing an effective medical supplement, herbal medicine seems to be the most popular method [10]. The evidence shows that more than 400 herbal medications, especially spices, could be helpful in the glycemic control of diabetes patients [10, 11].

One of these plants, ginger (Zingiber officinale Rosco), is thought to help manage glucose levels considerably [10, 12]. Ginger has various health benefits in addition to glycemic management, and it is widely utilized in traditional Chinese, Ayurvedic, and Tibb Unani medicine [13‐15].

The antioxidant, anti-tumor, and anti-inflammatory properties of ginger are thought to be responsible for its health benefits [13, 14]. Ginger was indicated in many clinical investigations to improve fasting blood glucose (FBG), serum insulin, and insulin resistance [12, 16], as well as lower urea and creatinine levels [17‐19]. Other evidence revealed that ginger might effectively treat renal disorders [20]. All of them, however, are still under study and will need more thorough research to validate or refute the present notion [20].

This study aimed to assess the effect of ginger supplementation on glycemic control, renal function, and prooxidant-antioxidant balance in patients with diabetes and ESRD undergoing hemodialysis, taking into account all of the potential benefits of ginger in glycemic control and improving health outcomes [13, 14, 21, 22].

A total of 44 people aged between 31 and 77 years old were randomly assigned to either a ginger supplement (n = 22) or a placebo (n = 22) in a randomized placebo-controlled double-blind study. The intention-to-treat analysis included 41 of the 44 randomized patients. Table 1 shows the baseline characteristics of study participants; Obviously, there were no significant variations in age, gender, height, weight, BMI, dialysis frequency, or medicines (p > 0.05).

To the best of our knowledge, this is the first clinical trial aimed to investigate the effects of ginger supplementation on diabetic ESRD patients undergoing hemodialysis. According to current findings, short-term ginger supplementation for eight weeks in this group of patientsmay improve glycemic control. Besides, it mar have beneficial effects on some renal function parameters. Furthermore, the results showed no significant changes in energy, macronutrients, and micronutrient intake between the two groups at the end of the study compared to the beginning, indicating the diet was not a notable confounder in this study.

Blood glucose control in diabetic patients, particularly those on hemodialysis, may be difficult under the shadow of of hypoglycemia. Moreover, these patients' treatment choices are limited due to impaired renal function and the buildup of medication metabolites in the body [27]. Current results on the efficacy of ginger in lowering FBG and HOMA-IR (by 24% and 28%) are in line with the previous research [16]. The coefficient of variation (CV%) relative to the mean for FBS is 32% in the ginger group versus 23% in the placebo group. Also, the calculated ranges (i.e., minimum and maximum) in the ginger and placebo groups at the baseline are 185 and 135, and at the endpoint, they are 120 and 129, respectively. In this regard, we used our randomization process to make the group similar by stratifying on FBS categories. Afterward, we adjusted the dissimilarities between groups in the multivariable statistical modeling, ANCOVA. Based on our literature review, only one study was conducted to assess the hypoglycemic effect of ginger in ESRD patients [16], which indicated the positive effects of ginger in reducing FBS; besides, some animal studies demonstrated that ginger decreased blood glucose levels in kidney dysfunction [28‐32]. No significant impact on serum insulin levels was observed in the present study that was in accordance with the study done by Mozaffari-Khosravi et al., showed no significant effect of ginger on serum insulin levels in diabeticpatients [33]. Mahluji et al., on the other hand, discovered that eating two grams of ginger per day for two months in patients with type 2 diabetes had no impact on FBG but may lower blood insulin and insulin resistance [34].

The phenols, polyphenols, and flavonoids in ginger are thought to have hypoglycemic effects [35]. Ginger seems to help with insulin resistance by increasing GLUT4 translocation from the cytosol to the cell membrane [36]. Besides, ginger's inhibition of the hepatic glucose 6-phosphatase enzyme activity may lower blood glucose levels [37]. While studies on the effect of ginger supplementation on insulin levels in the blood have produced mixed results [38], some active constituents of ginger, such as 6-gingerol and 6-shogaol, may affect insulin resistance by upregulating adiponectin and peroxisome proliferator-activated receptor, which improves insulin sensitivity and glycemic control [39]. It has been established that the PPAR-γ agonists increase plasma levels of adiponectin in diabetes [40], and adiponectin enhances insulin sensitivity by reducing inflammation and oxidative stress [25, 41]. Hence, ginger may have beneficial effects on maintaining the homeostasis of glucose.

In this study, ginger reduced urea levels consistent with prior animal studies [42‐44]. This suggests that ginger may have some mild renoprotectve effects in diabetic patients with ESRD; however, there are very few human studies to support this claim. In this study, serum creatinine levels decreased by 8% in the ginger group, but the differences did not reach a significant level.These findings were consistent with some animal studies [31, 45, 46]. in which, the administration of ginger caused to lower serum creatinine levels [43, 47, 48]. This disagreement may be due to the differences in the amount and form of ginger administered in animal studies.

The renoprotective properties in lowering the serum levels of urea may be attributed to polyphenols and flavonoids in ginger [49]. In earlier studies, free radicals have been linked to renal failure in various ways [18, 50]. Ginger improves kidney function by scavenging free radicals [51]. According to Uz et al., ginger administration raised the levels of several antioxidant enzymes (serum superoxide dismutase (SOD) and glutathione peroxidase (GPx)) that defend against oxygen free radicals in rats suffering from Renal Ischemia/Reperfusion damage [18]. Urea can lead to functional changes in the kidney by increasing the production of free radicals and apoptosis [52]. While creatinine is filtered at the glomerulus and eliminated from the plasma by the kidneys, urea reabsorption is hypothesized to occur as a consequence of water reabsorption [53]. Therefore, ginger might have influenced creatinine excretion and urea reabsorption in the nephrons.

In a live organism, there are several antioxidant and oxidant factors, and a disturbance of the pro-oxidant/antioxidant equilibrium may result in tissue harm. The pro-oxidant antioxidant balance showed substantial promise for mortality prediction in individuals with chronic renal disease, and oxidative stress in diabetic patients were much higher than in healthy individuals [54‐56]. According to the current study, ginger caused lowering serum levels of PAB by 10%, although the differences between the two study groups were not statistically significant. The presence of polyphenols and flavonoids in ginger is thought to be responsible for its antioxidant properties [57]. No study was found about the effects of ginger on serum levels of PAB in patients undergoing hemodialysis. However, two human studies have been conducted on the effect of ginger on malondialdehyde (MDA) levels in patients with ESRD, and their results were contradictory. Although Imani et al. observed no effect of ginger on serum levels of MDA in peritoneal dialysis patients, ginger caused lower MDA levels in ESRD patients in the Seddik et al. study [16, 58].

Hyperglycemia increases the production of Reactive oxygen species (ROS), and ginger can reduce ROS levels by lowering serum blood glucose concentrations [42]. In addition, the antioxidant activity of ginger could be explained through the following possible mechanisms: 1) Increasing nuclear factor erythroid-related factor 2 (Nrf2) signaling by ginger's bioactive compounds (such as 6-shogaol) [59]. 2) Inhibition of protein kinase C [60]. 3) Inhibition of the polyol pathway [61]. 4) Reducing the production of advanced glycation products (AGEs) can reduce these compounds' destructive effects in increasing ROS production [62].

The results of the present study indicated that diabetic ESRD patients undergoing hemodialysis who were 18 years of age or older and with no defined history of acute gastrointestinal diseases, thyroid abnormalities, gallstones, or ginger products sensitivity may benefit from ginger supplementation. This study was the first one that evaluated the PAB in kidney disease patients. Moreover, a high percentage of patients' adherence to the study protocol can be considered as another study's strength, indicating negligible side effects of ginger in this group of patients. We did not assess glycosylated hemoglobin, components of the body's antioxidant system such as antioxidant enzymes of SOD, GPx, catalase, serum levels of MDA, and some indicators of the body's oxidant status in urine due to financial constraints. Moreover, it seems that the study's short supplementation duration of eight weeks and the small number of patients included in the study were another drawbacks and the causes of certain non-statistically significant alterations at the conclusion.

In this study, ginger could result in lower blood glucose levels, enhanced insulin sensitivity, and lower serum urea levels with no effect on prooxidant-antioxidant balance (PAB) in patients with diabetes and ESRD who were receiving hemodialysis. However, further studies with a more extended intervention period and various doses and formes of ginger are needed.