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Journal of Cancer Research and Clinical Oncology

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01.05.2010 | Original Paper

The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer

verfasst von: Torben F. Hansen, Karen-Lise G. Spindler, Karen A. Lorentzen, Dorte A. Olsen, Rikke F. Andersen, Jan Lindebjerg, Ivan Brandslund, Anders Jakobsen

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 5/2010

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Abstract

Purpose

The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) −460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue.

Methods

Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT–PCR and protein analysis by ELISA.

Results

The T-allele in the −460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either.

Conclusion

The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.

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Titel: The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer
verfasst von: Torben F. Hansen
Karen-Lise G. Spindler
Karen A. Lorentzen
Dorte A. Olsen
Rikke F. Andersen
Jan Lindebjerg
Ivan Brandslund
Anders Jakobsen
Publikationsdatum: 01.05.2010
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 5/2010
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-009-0714-1

Springer Medizin

The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer