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Journal of Cancer Research and Clinical Oncology

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01.12.2007 | Original Paper

The influence of different microenvironments on melanoma invasiveness and microcirculation patterns: an animal experiment study in the mouse model

verfasst von: Baocun Sun, Shiwu Zhang, Danfang Zhang, Yanjun Gu, Wenchao Zhang, Xiulan Zhao

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 12/2007

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Abstract

Object

To investigate the influence of different microenvironments on tumor microcirculation patterns and invasiveness capability.

Methods

Melanoma B16 cells were injected into the abdominal cavity and skeletal muscle of C57 mice synchronously. CK-18 expression in melanoma was assessed to distinguish the malignant phenotype of tumors in the abdominal cavity from that in the skeletal muscle. HIF-1α, MMP-2 and MMP-9 expression and mRNA levels of MMP-2 and MMP-9 was detected to compare the mRNA levels of MMP-2 and MMP-9 from the two microenvironments. Cells positive for each immunohistochemical stain and the vessels representative of each type of microcirculation pattern were counted in two microenvironments.

Results

CK-18 and HIF-1α expression of melanoma were significantly higher in the skeletal muscle than in the abdominal cavity (P < 0.05). Compared with the abdominal cavity, melanoma cells in the skeletal muscle overexpressed MMP-2 and MMP-9 (P < 0.05). Real time-PCR results also showed that MMP-2 and MMP-9 mRNA levels of melanoma were higher in the skeletal muscle than in the abdominal cavity (P < 0.05). VM channels and endothelium-dependent vessels were the major microcirculation pattern in the skeletal muscle and in the abdominal cavity, respectively.

Conclusion

Different microenvironments affect invasiveness and blood supply patterns of melanoma.

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Titel: The influence of different microenvironments on melanoma invasiveness and microcirculation patterns: an animal experiment study in the mouse model
verfasst von: Baocun Sun
Shiwu Zhang
Danfang Zhang
Yanjun Gu
Wenchao Zhang
Xiulan Zhao
Publikationsdatum: 01.12.2007
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 12/2007
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-007-0245-6

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