Background
Methods
Groups
Ethics
Inclusion criteria
Exclusion criteria
Collection of gastric specimens and histopathologic examination
Questionnaire
Oral examination
Collection of dental biofilm and saliva specimens
Preparation of DNA from dental biofilm and saliva specimens and amplification of 16S rRNA and 23S rRNA genes
Evaluation of Streptococcus mutans and Lactobacilli levels in saliva samples
Evaluation of salivary flow rate, pH, and buffering capacity
Statistical analysis
Results
Detection rates of H.pylori in gastric, dental biofilm and saliva samples
Dyspeptic children who underwent endoscopy (Study group) (n = 70) | Children without dyspepsia (Control Group) (n = 30) |
P
| |||
---|---|---|---|---|---|
Endoscopic Examination Results | Gastric H.pylori (−) (n = 12, 17%) | Gastric H.pylori (+) (n = 58, 83%) | No endoscopic examination | ||
Saliva | 16 S RNA (+) | 10 (83.3%) | 46 (79.3%) | 14 (46.7%)
*
|
a0.004 |
23 S RNA (+) | 10 (83.3%) | 55 (94.8%) | 25 (83.3%) |
a0.167 | |
16 S RNA+ 23 S RNA (+) | 9 (75.0%) | 44 (75.9%) | 12 (40.0%)
*
|
a0.003 | |
Dental Biofilm | 16 S RNA (+) | 10 (83.3%) | 49 (84.5%) | 30 (100%) |
a0.070 |
23 S RNA (+) | 10 (83.3%) | 53 (91.4%) | 5 (16.7%)
*
|
a0.001 | |
16 S RNA+ 23 S RNA (+) | 8 (66.7%) | 45 (77.6%) | 5 (16.7%)
*
|
a0.001 | |
Halitosis (BANA Test Results) | Negative | (n = 1, 8.3%) | (n = 4, 6.9%) | (n = 12, 40%) |
a0.001 |
Weak Positive | (n = 5, 41.7%) | (n = 34, 58.6%) | (n = 14, 46.7%) | ||
Positive | (n = 6, 50%) | (n = 20, 34.5%) | (n = 4, 13.3%)
*
| ||
Oral Findings | DMFT | 4.5 ± 4.86 | 5.31 ± 3.99
**
| 2.19 ± 2.14 |
b0.001 |
DMFS | 7.1 ± 4.7 | 7.62 ± 7.7
**
| 2.27 ± 2.16 |
b0.001 | |
dmft | 4.67 ± 2.58 | 3 ± 2.79
**
| 7.41 ± 4.68 |
b0.001 | |
dmfs | 9.5 ± 8.57 | 5.32 ± 5.38
**
| 15.59 ± 10.91 |
b0.001 | |
Gingival Index | 0.42 ± 0.77 | 0.27 ± 0.49 | 0.04 ± 0.09 |
b0.113 | |
Plaque Index | 0.63 ± 0.36
**
| 0.78 ± 0.62
**
| 0.34 ± 0,3 |
b0.001 | |
Salivary Flow Rate | 5.04 ± 2.17 | 6.55 ± 2.83 | 5.85 ± 2.17 |
c0.139 | |
Saliva pH | 7.4 ± 0.32 | 7.45 ± 0.34 | 7.61 ± 0.38 |
c0.096 |
Halitosis
Dental Biofilm |
p
| Saliva |
p
| ||||
---|---|---|---|---|---|---|---|
16S + 23S RNA (+) (n = 58) n (%) | 16S + 23S RNA (−) (n = 42) n (%) | 16S + 23S RNA (+) (n = 65) n (%) | 16S + 23S RNA (−) (n = 35) n (%) | ||||
Halitosis (BANA Test Results) | Negative | 5 (8.6%)
*
| 12 (28.6%) |
a0.007 | 6 (9.2%)
**
| 11 (31.4%) |
a0.014 |
Weak Positive | 30 (51.7%)
*
| 23 (54.8%) |
a0.007 | 36 (55.4%)
**
| 17 (48.6%) |
a0.014 | |
Positive | 23 (39.7%)
*
| 7 (16.7%) |
a0.007 | 23 (35.4%)
**
| 7 (20%) |
a0.014 | |
Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | ||||
Oral Findings | DMFT | 5.22 ± 4.2
*
| 3.03 ± 2.98 |
b0.005 | 4.72 ± 3.85 | 3.53 ± 3.91 |
b0.092 |
DMFS | 7.43 ± 7.91
*
| 3.19 ± 3.06 |
b0.001 | 6.67 ± 7.43
*
| 3.73 ± 4.38 |
b0.024 | |
dmft | 4.1 ± 3.77 | 6.1 ± 4.5 |
b0.066 | 3.94 ± 4.13
*
| 6.69 ± 3.95 |
b0.004 | |
dmfs | 7.33 ± 7.24
*
| 13.13 ± 11.05 |
b0.023 | 7.26 ± 8
*
| 14.35 ± 10.52 |
b0.002 | |
Gingival Index | 0.27 ± 0.49 | 0.15 ± 0.44 |
b0.059 | 0.27 ± 0.51
*
| 0.12 ± 0.36 |
b0.005 | |
Plaque Index | 0.72 ± 0.6
*
| 0.51 ± 0.43 |
b0.049 | 0.71 ± 0.59 | 0.50 ± 0.42 |
b0.088 | |
Salivary Flow Rate | 6.48 ± 2.93 | 5.71 ± 2.02 |
c0.146 | 6.48 ± 2.89 | 5.57 ± 1.85 |
c0.097 | |
Saliva pH | 7.42 ± 0.32
*
| 7.59 ± 0.37 |
c0.019 | 7.42 ± 0.31
*
| 7.62 ± 0.39 |
c0.009 |
Oral parameters
Odds Ratio | %95 CI |
p
| |||
---|---|---|---|---|---|
Lower | Upper | ||||
Step 1 | DMFT | 0.098 | 0.005 | 1.829 | 0.120 |
DMFS | 10.000 | 0.619 | 161.469 | 0.105 | |
dmfs | 0.906 | 0.828 | 0.992 | 0.033 | |
Plaque Index | 1.505 | 0.421 | 5.374 | 0.529 | |
pH | 0.055 | 0.005 | 0.651 | 0.021* | |
Step 2 | DMFT | 1.007 | 0.005 | 1.671 | 0.105 |
DMFS | 11.294 | 0.684 | 186.402 | 0.090 | |
dmfs | 0.904 | 0.826 | 0.990 | 0.029* | |
pH | 0.053 | 0.005 | 0.630 | 0.020* |
Odds Ratio | %95 CI |
p
| |||
---|---|---|---|---|---|
Lower | Upper | ||||
Step 1 | DMFS | 1.283 | 0.929 | 1.772 | 0.130 |
dmft | 1.023 | 0.679 | 1.540 | 0.913 | |
dmfs | 0.869 | 0.715 | 1.056 | 0.157 | |
Gingival Index | 2.176 | 0.231 | 20.507 | 0.497 | |
pH | 0.492 | 0.047 | 5.114 | 0.552 | |
Step 2 | DMFS | 1.279 | 0.934 | 1.751 | 0.125 |
dmfs | 0.877 | 0.794 | 0.968 | 0.009* | |
Gingival Index | 2.173 | 0.230 | 20.520 | 0.498 | |
pH | 0.488 | 0.047 | 5.035 | 0.547 | |
Step 3 | DMFS | 1.270 | 0.933 | 1.730 | 0.129 |
dmfs | 0.872 | 0.790 | 0.963 | 0.007* | |
Gingival Index | 1.868 | 0.204 | 17.150 | 0.581 | |
Step 4 | DMFS | 1.282 | 0.942 | 1.744 | 0.114 |
dmfs | 0.864 | 0.785 | 0.950 | 0.003* |