Erschienen in:
24.03.2020 | Original Article
The Lectin Isolated from the Alga Hypnea cervicornis Promotes Antinociception in Rats Subjected to Zymosan-Induced Arthritis: Involvement of cGMP Signalization and Cytokine Expression
verfasst von:
Pedro Henrique de Souza Ferreira Bringel, Gabriela Fernandes Oliveira Marques, Maria Gleiciane de Queiroz Martins, Mayara Torquato Lima da Silva, Clareane Avelino Simplício Nobre, Kyria Santiago do Nascimento, Benildo Sousa Cavada, Rondinelle Ribeiro Castro, Ana Maria Sampaio Assreuy
Erschienen in:
Inflammation
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Ausgabe 4/2020
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Abstract
This study investigated the effects of the alga lectin Hypnea cervicornis agglutinin (HCA) on rat zymosan-induced arthritis (ZyA). Zymosan (50–500 μg/25 μL) or sterile saline (Sham) was injected into the tibio-tarsal joint of female Wistar rats (180–200 g). Arthritic animals received morphine (4 mg/kg, intraperitoneal), indomethacin (5 mg/kg, intraperitoneal), or 2% lidocaine (100 μL, subcutaneous). HCA (0.3–3 mg/kg) was administered by intravenous route 30 min before or 2 h after zymosan. 1H-[1,2,4]oxadiazolo[4,3-a]-quinoxalin-1-one (ODQ, 4 μg, intra-articular) was given 30 min prior HCA. Hypernociception was measured every hour until 6 h, time in which animals were sacrificed for evaluation of leukocytes of the intra articular fluid and gene expression of TNF-α, IL-1, IL-10, and iNOS in the joint tissues using PCR techniques. Hypernociception was responsive to morphine and indomethacin, and its threshold was not altered by lidocaine. The post-treatment of HCA reduced both hypernociception and leukocyte influx. This antinociceptive effect was abolished either by ODQ and glibenclamide. HCA also reduced gene expression of iNOS and TNF-α. In conclusion, the antinociceptive effect of HCA in ZyA involves cyclic GMP signalization and selective modulation of cytokine expression.