Background
Content | Modified Gingyo-san (MGS)aAmount (g)b | Gingyo-san (GS) Amount (g)b |
---|---|---|
Lonicerae Flos | 4.26 | 30.0 |
Forsythiae Fructus | 4.26 | 30.0 |
Glycyrrhizae Radix | 2.56 | 15.0 |
Platycodi Radix | 2.56 | 18.0 |
Menthae Herba | 2.56 | 18.0 |
Arctii Fructus | 2.14 | 18.0 |
Schizonepetae Spica | 1.7 | 12.0 |
Fermented soybean | 2.14 | 12.0 |
Lophatherum Herba | 1.7 | 15.0 |
Antelope Horns | 0.13 | - |
Methods
Microbial strains and culture conditions
Microorganism | Description |
---|---|
Gram-positive bacteria | |
Staphylococcus aureus JCM2874 | Quality control strain for susceptibility test, Methicillin-susceptible |
Staphylococcus aureus N315 | Methicillin-resistant S. aureus |
Streptococcus pneumoniae ATCC49619 | Quality control strain for susceptibility test, penicillin-susceptible |
Streptococcus pneumoniae 19F | Clinical isolate, penicillin-resistant S. pneumoniae, serotype 19F |
Streptococcus pyogenes JCM5674 | Type strain |
Enterococcus faecalis ATCC29212 | Quality control strain for susceptibility test |
Bacillus subtilis ATCC6633 | Control strain for various assays |
Gram-negative bacteria | |
Escherichia coli ATCC25922 | Quality control strain for susceptibility test |
Escherichia coli C | Host of bacteriophage φX174 |
Pseudomonas aeruginosa ATCC27853 | Quality control strain for susceptibility test |
Haemophilus influenzae ATCC49247 | Quality control strain for susceptibility test |
Serratia marcescens ATCC13880 | Type strain |
Fungi | |
Candida albicans ATCC10231 | Quality control strain for various assays |
Bacteriophage | |
φX174 | Virulent phage for E. coli C |
Disk diffusion method
Evaluation of growth inhibitory activity
Plaque assay
Cell proliferation assay
Statistical analysis
Results and discussion
Antimicrobial activity of MGS
Strain | Inhibitory zone (mm)b |
---|---|
Staphylococcus aureus JCM2874 | - |
Staphylococcus aureus N315 | - |
Streptococcus pneumoniae ATCC49619 | 10.0 |
Streptococcus pneumoniae 19F | 10.0 |
Streptococcus pyogenes JCM5674 | 8.5 |
Enterococcus faecalis ATCC29212 | - |
Bacillus subtilis ATCC6633 | - |
Escherichia coli ATCC25922 | - |
Pseudomonas aeruginosa ATCC27853 | - |
Haemophilus influenzae ATCC49247 | - |