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International Urogynecology Journal

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01.01.2010 | Original Article

The role of calpain–calpastatin system in the development of stress urinary incontinence

verfasst von: Yuzhong Wu, Li Zhang, Hangmei Jin, Jianhong Zhou, Zhenwei Xie

Erschienen in: International Urogynecology Journal | Ausgabe 1/2010

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Abstract

Introduction and hypothesis

The objective of this study is to investigate the expression of calpain-1, calpain-2, and calpastatin in the human periurethral vaginal tissues and to show the potential link between calpain system and stress urinary incontinence (SUI).

Methods

The periurethral vaginal tissues of 39 women with SUI and 31 women without SUI were collected to detect the expressions of calpains and calpastatin by using semi-quantitative competitive reverse transcription-polymerase chain reaction and Western blotting.

Results

There were no significant differences on the expressions of calpain-1 at the levels of messenger RNA (mRNA) and protein in both groups (P > 0.05), but the patients with SUI had significantly higher levels of calpain-2 mRNA and protein than the control (P < 0.05); and the mRNA expressions of calpastatin in women with SUI were significantly higher than the control (P < 0.05), while the protein expressions were significantly lower when compared to the control (P < 0.01).

Conclusions

Overexpression of calpain-2 and low expression of calpastatin may involve in the pathological development of SUI.

Hunskaar S, Lose G, Sykes D, Voss S (2004) The prevalence of urinary incontinence in women in four European countries. BJU Int 93:324–330CrossRefPubMed

Diokno AC, Burgio K, Fultz H, Kinchen KH, Obenchain R, Bump RC (2003) Prevalence and outcomes of continence surgery in community dwelling women. J Urol 170:507–511CrossRefPubMed

MacLennan AH, Taylor AW, Wilson DH, Wilson D (2000) The prevalence of pelvic floor disorders and their relationship to gender, age, parity and mode of delivery. BJOG 107:1460–1470CrossRefPubMed

Duong TH, Korn AP (2001) A comparison of urinary incontinence among African American, Asian, Hispanic, and white women. Am J Obstet Gynecol 184:1083–1086CrossRefPubMed

Viktrup L, Lose G (2001) The risk of stress incontinence 5 years after first delivery. Am J Obstet Gynecol 185:82–87CrossRefPubMed

Delancey JO, Ashton-Miller JA (2004) Pathophysiology of adult urinary incontinence. Gastroenterology 126:S23–S32CrossRefPubMed

Takahashi S, Homma Y, Fujishiro T, Hosaka Y, Kitamura T, Kawabe K (2000) Electromyographic study of the striated urethral sphincter in type 3 stress incontinence: evidence of myogenic-dominant damages. Urology 56:946–950CrossRefPubMed

Hale DS, Benson JT, Brubaker L, Heidkamp MC, Russell B (1999) Histologic analysis of needle biopsy of urethral sphincter from women with normal and stress incontinence with comparison of electromyographic findings. Am J Obstet Gynecol 180:342–348CrossRefPubMed

Boreham MK, Miller RT, Schaffer JI, Word RA (2001) Smooth muscle myosin heavy chain and caldesmon expression in the anterior vaginal wall of women with and without pelvic organ prolapse. Am J Obstet Gynecol 185:944–952CrossRefPubMed

10.

DeLancey JO (1994) Structural support of the urethra as it relates to stress urinary incontinence: the hammock hypothesis. Am J Obstet Gynecol 170:1713–1720PubMed

11.

Goll DE, Thompson VF, Li H, Wei W, Cong J (2003) The calpain system. Physiol Rev 83:731–801PubMed

12.

Mellgren RL (1987) Calcium-dependent proteases: an enzyme system active at cellular membranes? FASEB J 1:110–115PubMed

13.

Ono Y, Sorimachi H, Suzuki K (1998) Structure and physiology of calpain, an enigmatic protease. Biochem Biophys Res Commun 245:289–294CrossRefPubMed

14.

Porn-Ares MI, Samali A, Orrenius S (1998) Cleavage of the calpain inhibitor, calpastatin, during apoptosis. Cell Death Differ 5:1028–1033CrossRefPubMed

15.

Lecker SH, Goldberg AL, Mitch WE (2006) Protein degradation by the ubiquitin-proteasome pathway in normal and disease states. J Am Soc Nephrol 17:1807–1819CrossRefPubMed

16.

Carragher NO (2006) Calpain inhibition: a therapeutic strategy targeting multiple disease states. Curr Pharm Des 12:615–638CrossRefPubMed

17.

Huang Y, Wang KK (2001) The calpain family and human disease. Trends Mol Med 7:355–362CrossRefPubMed

18.

Perrin BJ, Huttenlocher A (2002) Calpain. Int J Biochem Cell Biol 34:722–725CrossRefPubMed

19.

Zatz M, Starling A (2005) Calpains and disease. N Engl J Med 352:2413–2423CrossRefPubMed

20.

Liu J, Liu MC, Wang KKW (2008) Calpain in the CNS: from synaptic function to neurotoxicity. Sci Signal 1:re1CrossRefPubMed

21.

Chen GD, Chen YC, Lin LY (2004) Different expression of calpains in the anterior vaginal wall of women with and without uterovaginal prolapse. J Soc Gynecol Investig 11:113–117CrossRefPubMed

22.

Abrams P, Cardozo L, Fall M, Griffiths D, Rosier P, Ulmsten U, van Kerrebroeck P, Victor A, Wein A (2002) The standardisation of terminology of lower urinary tract function: report from the Standardisation Sub-committee of the International Continence Society. Am J Obstet Gynecol 187:116–126CrossRefPubMed

23.

Nuzzi PA, Senetar MA, Huttenlocher A (2007) Asymmetric localization of calpain 2 during neutrophil chemotaxis. Mol Biol Cell 18:795–805CrossRefPubMed

24.

Galvez AS, Diwan A, Odley AM, Hahn HS, Osinska H, Melendez JG, Robbins J, Lynch RA, Marreez Y, Dorn GW (2007) Cardiomyocyte degeneration with calpain deficiency reveals a critical role in protein homeostasis. Circ Res 100:1071–1078CrossRefPubMed

25.

Weber H, Jonas L, Hühns S, Schuff-Werner P (2004) Dysregulation of the calpain-calpastatin system plays a role in the development of cerulein-induced acute pancreatitis in the rat. Am J Physiol Gastrointest Liver Physiol 286:G932–G941CrossRefPubMed

26.

Borello-France DF, Handa VL, Brown MB, Goode P, Kreder K, Scheufele LL, Weber AM (2007) Pelvic-floor muscle function in women with pelvic organ prolapse. Phys Ther 87:399–407PubMed

27.

Zhang W, Lane RD, Mellgren RL (1996) The major calpain isozymes are long-lived proteins. Design of an antisense strategy for calpain depletion in cultured cells. J Biol Chem 271:18825–18830CrossRefPubMed

28.

Shi Y, Melnikov VY, Schrier RW, Edelstein CL (2000) Downregulation of the calpain inhibitor protein calpastatin by caspases during renal ischemia-reperfusion. Am J Physiol Renal Physiol 279:F509–F517PubMed

Titel: The role of calpain–calpastatin system in the development of stress urinary incontinence
verfasst von: Yuzhong Wu
Li Zhang
Hangmei Jin
Jianhong Zhou
Zhenwei Xie
Publikationsdatum: 01.01.2010
Verlag: Springer-Verlag
Erschienen in: International Urogynecology Journal / Ausgabe 1/2010
Print ISSN: 0937-3462
Elektronische ISSN: 1433-3023
DOI: https://doi.org/10.1007/s00192-009-0988-5

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The role of calpain–calpastatin system in the development of stress urinary incontinence

Abstract

Introduction and hypothesis

Methods

Results

Conclusions

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