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Erschienen in:
01.07.2011 | Basic Science Research
The Role of Receptor-Interacting Protein 140 in the Accumulation of Fat in Ovariectomised Rats
Erschienen in: Obesity Surgery | Ausgabe 7/2011
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Background
Receptor-interacting protein 140 (RIP140) is a corepressor for nuclear receptors with an important role in the inhibition of energy expenditure. Postmenopausal women have increased white adipose tissue (WAT), and excessive accumulation of adipose tissue (obesity) implies a health risk. The aim of the present work was to investigate the time course of RIP140 expression in WAT during the development of ovariectomy (OVX)-induced obesity in rats.
Methods
OVX was performed in female Sprague–Dawley (SD) rats 8 weeks old. Body weight and food intake were determined once a week. WAT of sham-operated, OVX and OVX plus 17β-estradiol therapy (OVX/E2) female SD rats was weighed and used to analyse RIP140 and uncoupling protein 1 (UCP-1) expression by Western blot.
Results
Food intake and body weight were significantly increased during the 2–8 weeks after OVX. Even though body weight increased until killing, food intake progressively decreased from 9 to 16 weeks after OVX in rats. Meanwhile, increased WAT mass and decreased RIP140 expression in WAT were observed in OVX rats. In contrast, the expression of UCP-1, a key target gene of RIP140, in WAT of OVX rats was significantly higher than in sham-operated rats. All of these alterations caused by OVX were mostly reversed by the replacement of 17β-estradiol.
Conclusions
The down-regulation of RIP140 in WAT may play a compensatory role in OVX-induced obesity in rat.