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Brain Tumor Pathology

Erschienen in:

12.11.2022 | Original Article

Three-dimensional visualization of human brain tumors using the CUBIC technique

verfasst von: Yangyang Xu, Qi He, Mengqi Wang, Yang Wu, Yifeng Shi, Wei Wang, Jie Zhang

Erschienen in: Brain Tumor Pathology | Ausgabe 1/2023

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Abstract

Application of tissue clearing techniques on human brain tumors is still limited. This study was to investigate the application of CUBIC on 3D pathological studies of human brain tumors. Brain tumor specimens derived from 21 patients were cleared with CUBIC. Immunostaining was conducted on cleared specimens to label astrocytes, microglia and microvessels, respectively. All tumor specimens achieved transparency after clearing. Immunostaining and CUBIC are well compatible in a variety of human brain tumors. Spatial morphologies of microvessels, astrocytes and microglia of tumors were clearly visualized in 3D, and their 3D morphological parameters were easily quantified. By comparing the quantitative morphological parameters of microvessels among brain tumors of different malignancy, we found that mean vascular diameter was positively correlated with tumor malignancy. Our study demonstrates that CUBIC can be successfully applied to 3D pathological studies of various human brain tumors, and 3D studies of human brain tumors hold great promise in helping us better understand brain tumor pathology in the future.

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Titel: Three-dimensional visualization of human brain tumors using the CUBIC technique
verfasst von: Yangyang Xu
Qi He
Mengqi Wang
Yang Wu
Yifeng Shi
Wei Wang
Jie Zhang
Publikationsdatum: 12.11.2022
Verlag: Springer Nature Singapore
Erschienen in: Brain Tumor Pathology / Ausgabe 1/2023
Print ISSN: 1433-7398
Elektronische ISSN: 1861-387X
DOI: https://doi.org/10.1007/s10014-022-00445-2

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