Background
Methods
Preliminary experiment
Cell proliferation assay
Formal experiment
Cell culture and treatment
Compounds | Concentrations |
---|---|
Ticagrelor | 20 μM |
Clopidogrel | 20 μM |
LPS | 10 ng/mL |
CD14 | 1 μg/mL |
Cell proliferation assay
Western blot assay
RNA extraction and quantitative polymerase chain reaction (qPCR)
Name | Primer | Sequence |
---|---|---|
homo GAPDH | Forward | 5′- TCAAGAAGGTGGTGAAGCAGG − 3′ |
Reverse | 5′- TCAAAGGTGGAGGAGTGGGT − 3’ | |
Homo TNFα | Forward | 5′-CCCATGTTGTAGCAAACCCTC − 3’ |
Reverse | 5′-AGAGGACCTGGGAGTAGATGA − 3’ | |
Reverse | 5′-GTGCCTCTTTGCTGCTTTC − 3’ | |
Homo IL-1 | Forward | 5′-CGAATCTCCGACCACCACTA − 3’ |
Reverse | 5′-AGCCTCGTTATCCCATGTGT − 3’ | |
Homo IL-6 | Forward | 5′-GGTCCAGTTGCCTTCTCCC − 3’ |
Reverse | 5′-GTGCCTCTTTGCTGCTTTC − 3’ | |
Homo IL-2 | Forward | 5′-CAACTCCTGTCTTGCATTGC-3’ |
Reverse | 5′-TGTGAGCATCCTGGTGAGTT − 3’ | |
Homo IL-8 | Forward | 5′-GACATACTCCAAACCTTTCCACCCC-3’ |
Reverse | 5′-CAAAAACTTCTCCACAACCCTCTGC −3’ |