Background
Methods
Bacterial strains
Transcriptional expression analysis of blaNDM-1
Transformation and Conjugation assay
Replicon typing and plasmid stability analyses
Copy number determination of plasmid encoding blaNDM-1
Antimicrobial susceptibility testing and MIC determination
Typing of blaNDM-1 harboring isolates
Results
Strain ID | Date of Isolation | Patient’s Sex & Age | Sample origin | Ward |
bla
NDM-1 positive plasmids | Pulsotypes | |
---|---|---|---|---|---|---|---|
Name | Inc-type | ||||||
EC51 | 5-3-2013 | M-27 years | Stool | Medicine | PEC-51
| FIC | P1
|
EC54 | 11-3-2013 | M-26 years | Stool | Medicine | PEC-54
| A/C | P4
|
EC61 | 11-3-2013 | M-31 years | Surgical wound | Surgery | PEC-61
| K | P1
|
EC75 | 13-3-2013 | F-47 years | Stool | Medicine | PEC-75
| untypeable | P1
|
EC177 | 7-4-2013 | M-33 years | Urine | Medicine | PEC-177
| A/C | P3
|
EC178 | 7-4-2013 | F-12.5 years | Pus | Surgery | PEC-178
| FIC | P2
|
EC255 | 13-5-2013 | M-48 years | Pus | Surgery | PEC-255
| FIC | P2
|
EC355 | 27-6-2013 | M-10 years | Urine | Medicine | PEC-355
| untypeable | P2
|
EC456 | 6-7-2013 | F-25 years | Throat swab | ENT | PEC-456
| A/C | P5
|
EC472 | 9-7-2013 | F-62 years | Pus | Orthopedics | PEC-472
| K | P6
|
EC477 | 9-7-2013 | F-32 years | Ear swab | ENT | PEC-477
| FIC | P3
|
EC489 | 16-7-2013 | M-8.5 years | Pus | Surgery | PEC-489
| K | P2
|
EC492 | 19-7-2013 | M-11 years | Endotracheal aspirates | OPD | PEC-492
| untypeable | P6
|
EC571 | 15-8-2013 | F-21 years | Cerebrospinal fluid | Surgery | PEC-571
| FIC | P2
|
EC611 | 29-8-2013 | F-42 years | Blood | Surgery | PEC-611
| A/C | P2
|
EC639 | 7-9-2013 | M-20 years | Urine | Medicine | PEC-639
| FIC | P5
|
EC678 | 16-9-2013 | M-38 years | Pus | Surgery | PEC-678
| FIC | P4
|