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28.09.2016 | Urology - Original Paper

Transplantation of mature adipocyte-derived dedifferentiated fat cells for the treatment of vesicoureteral reflux in a rat model

verfasst von: Yuichiro Ikado, Daisuke Obinata, Taro Matsumoto, Yasutaka Murata, Koichiro Kano, Noboru Fukuda, Kenya Yamaguchi, Satoru Takahashi

Erschienen in: International Urology and Nephrology | Ausgabe 12/2016

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Abstract

Purpose

Autologous cells potentially provide an ideal injectable substance for management in vesicoureteral reflux (VUR). The aim of this study is to examine the effects of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on VUR in a rat bladder pressurization-induced VUR model.

Methods

To create VUR, Sprague–Dawley rats underwent urethral clamping and placement of cystostomy followed by intravesical pressurization. Rat DFAT cells (1 × 10⁶ cells, DFAT group, n = 5) or saline (control group, n = 5) was then injected into the bilateral vesicoureteral junctions. Two weeks later, VUR grade was evaluated on cystography. The number of apoptotic cells in the renal pelvic urothelium, the ureteral inner/outer diameter ratio and the area of connective tissue in the posterior bladder wall were measured.

Results

The reflux grade in the DFAT group was significantly lower than that in the control group. The number of apoptotic cells in the renal pelvic urothelium, ureteral inner/outer diameter ratio and connective tissue area in DFAT group were significantly lower in comparison with the control group.

Conclusions

DFAT cell transplantation improved VUR and exerted nephroprotective effects in a rat VUR model.

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Titel: Transplantation of mature adipocyte-derived dedifferentiated fat cells for the treatment of vesicoureteral reflux in a rat model
verfasst von: Yuichiro Ikado
Daisuke Obinata
Taro Matsumoto
Yasutaka Murata
Koichiro Kano
Noboru Fukuda
Kenya Yamaguchi
Satoru Takahashi
Publikationsdatum: 28.09.2016
Verlag: Springer Netherlands
Erschienen in: International Urology and Nephrology / Ausgabe 12/2016
Print ISSN: 0301-1623
Elektronische ISSN: 1573-2584
DOI: https://doi.org/10.1007/s11255-016-1426-5

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Abstract

Purpose

Methods

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Conclusions

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