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01.10.2013

Ultrasound-Targeted Microbubble Destruction Enhances Human β-Defensin 3 Activity Against Antibiotic-Resistant Staphylococcus Biofilms

verfasst von: Chen Zhu, Nianan He, Tao Cheng, Honglue Tan, Yongyuan Guo, Desheng Chen, Mengqi Cheng, Zhi Yang, Xianlong Zhang

Erschienen in: Inflammation | Ausgabe 5/2013

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Abstract

The infection of orthopedic implantation devices with Staphylococcus is a serious concern within the biomaterial community. Treatments are not always successful because of antibiotic-resistant bacteria and serious biofilm infections. Human β-defensin 3 (hBD-3) is considered to be the most promising class of defensin antimicrobial peptides and its effect on antibiotic-resistant Staphylococcus biofilms, combined with ultrasound (US)-targeted microbubble (MB) destruction (UTMD), has not been reported. In the study, we found that biofilm densities, the percentage of live cells and the viable counts of two tested Staphylococcus recovered from the biofilm were significantly decreased in the maximum concentration hBD-3 combined with UTMD compared with those of any other groups. Furthermore, results suggested that UTMD could also enhance 1MIC hBD-3 activity inhibiting the biofilm-associated genes expression of icaAD and the methicillin-resistance genes expression of MecA by simultaneously promoting the icaR expression. UTMD may have great potential for improving antibiotic activity against biofilm infections.

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Titel: Ultrasound-Targeted Microbubble Destruction Enhances Human β-Defensin 3 Activity Against Antibiotic-Resistant Staphylococcus Biofilms
verfasst von: Chen Zhu
Nianan He
Tao Cheng
Honglue Tan
Yongyuan Guo
Desheng Chen
Mengqi Cheng
Zhi Yang
Xianlong Zhang
Publikationsdatum: 01.10.2013
Verlag: Springer US
Erschienen in: Inflammation / Ausgabe 5/2013
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-013-9630-2

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