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Erschienen in:

26.04.2019 | Original Article

Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS)

verfasst von: J. Curtis Nickel, Alisa Stephens, J. Richard Landis, Chris Mullins, Adrie van Bokhoven, Jennifer T. Anger, A. Lenore Ackerman, Jayoung Kim, Siobhan Sutcliffe, Jaroslaw E. Krol, Bhaswati Sen, Jocelyn Hammond, Garth D. Ehrlich, The Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network

Erschienen in: World Journal of Urology | Ausgabe 2/2020

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Abstract

Purpose

To correlate the presence of fungi with symptom flares, pain and urinary severity in a prospective, longitudinal study of women with IC/BPS enrolled in the MAPP Research Network.

Methods

Flare status, pelvic pain, urinary severity, and midstream urine were collected at baseline, 6 and 12 months from female IC/BPS participants with at least one flare and age-matched participants with no reported flares. Multilocus PCR coupled with electrospray ionization/mass spectrometry was used for identification of fungal species and genus. Associations between “mycobiome” (species/genus presence, relative abundance, Shannon’s/Chao1 diversity indices) and current flare status, pain, urinary severity were evaluated using generalized linear mixed models, permutational multivariate analysis of variance, Wilcoxon’s rank-sum test.

Results

The most specific analysis detected 13 fungal species from 8 genera in 504 urine samples from 202 females. A more sensitive analysis detected 43 genera. No overall differences were observed in fungal species/genus composition or diversity by flare status or pain severity. Longitudinal analyses suggested greater fungal diversity (Chao1 Mean Ratio 3.8, 95% CI 1.3–11.2, p = 0.02) and a significantly greater likelihood of detecting any fungal species (OR = 5.26, 95% CI 1.1–25.8, p = 0.04) in high vs low urinary severity participants. Individual taxa analysis showed a trend toward increased presence and relative abundance of Candida (OR = 6.63, 95% CI 0.8–58.5, p = 0.088) and Malassezia (only identified in ‘high’ urinary severity phenotype) for high vs low urinary symptoms.

Conclusion

This analysis suggests the possibility that greater urinary symptom severity is associated with the urinary mycobiome urine in some females with IC/BPS.

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Titel: Urinary fungi associated with urinary symptom severity among women with interstitial cystitis/bladder pain syndrome (IC/BPS)
verfasst von: J. Curtis Nickel
Alisa Stephens
J. Richard Landis
Chris Mullins
Adrie van Bokhoven
Jennifer T. Anger
A. Lenore Ackerman
Jayoung Kim
Siobhan Sutcliffe
Jaroslaw E. Krol
Bhaswati Sen
Jocelyn Hammond
Garth D. Ehrlich
The Multidisciplinary Approach to the Study of Chronic Pelvic Pain (MAPP) Research Network
Publikationsdatum: 26.04.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: World Journal of Urology / Ausgabe 2/2020
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-019-02764-0

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