Background
Materials and methods
Materials
Cell cultures and transfection
Western blotting
Quantitative real-time RT-PCR
CCK-8 assay
Transwell invasion assay
Wound healing assay
Cell cycle assay
In vivo tumor growth and treatment
Statistical analysis
Results
Recombinant plasmid pcDNA3.1-RONΔ160 stably transfected into MGC-803 cells induces morphological changes
RONΔ160 improves the proliferation of MGC-803 cells
RONΔ160 increases the invasive capacity of MGC-803 cells
RONΔ160 promotes the motility of MGC-803 cells
RONΔ160 regulates the cell cycle activities of MGC-803 cells
Overexpression of RONΔ160 promotes tumor growth in vivo and its specific small molecule inhibitor can inhibit tumor growth
RONΔ160 Group*
|
CP-I Group
#
|
MGC-803 Group
|
Empty vector control
| |
---|---|---|---|---|
Tumor volume (mm3) | 2703.79 ± 92.66 | 1743.77 ± 119.75 | 1176.36 ± 149.13 | 1273.17 ± 316.23 |
Tumor weight (g) | 1.93 ± 0.11 | 1.21 ± 0.33 | 0.89 ± 0.29 | 0.91 ± 0.41 |
Tumor incubation period (days) | 12 ± 1 | 14.5 ± 1.5 | 20 ± 1.5 | 19.7 ± 2.52 |