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30.01.2016 | Original Article

Visual detection of Ebola virus using reverse transcription loop-mediated isothermal amplification combined with nucleic acid strip detection

verfasst von: Changping Xu, Hualei Wang, Hongli Jin, Na Feng, Xuexing Zheng, Zengguo Cao, Ling Li, Jianzhong Wang, Feihu Yan, Lina Wang, Hang Chi, Weiwei Gai, Chong Wang, Yongkun Zhao, Yan Feng, Tiecheng Wang, Yuwei Gao, Yiyu Lu, Songtao Yang, Xianzhu Xia

Erschienen in: Archives of Virology | Ausgabe 5/2016

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Abstract

Ebola virus (species Zaire ebolavirus) (EBOV) is highly virulent in humans. The largest recorded outbreak of Ebola hemorrhagic fever in West Africa to date was caused by EBOV. Therefore, it is necessary to develop a detection method for this virus that can be easily distributed and implemented. In the current study, we developed a visual assay that can detect EBOV-associated nucleic acids. This assay combines reverse transcription loop-mediated isothermal amplification and nucleic acid strip detection (RT-LAMP-NAD). Nucleic acid amplification can be achieved in a one-step process at a constant temperature (58 °C, 35 min), and the amplified products can be visualized within 2-5 min using a nucleic acid strip detection device. The assay is capable of detecting 30 copies of artificial EBOV glycoprotein (GP) RNA and RNA encoding EBOV GP from 10² TCID₅₀ recombinant viral particles per ml with high specificity. Overall, the RT-LAMP-NAD method is simple and has high sensitivity and specificity; therefore, it is especially suitable for the rapid detection of EBOV in African regions.

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Titel: Visual detection of Ebola virus using reverse transcription loop-mediated isothermal amplification combined with nucleic acid strip detection
verfasst von: Changping Xu
Hualei Wang
Hongli Jin
Na Feng
Xuexing Zheng
Zengguo Cao
Ling Li
Jianzhong Wang
Feihu Yan
Lina Wang
Hang Chi
Weiwei Gai
Chong Wang
Yongkun Zhao
Yan Feng
Tiecheng Wang
Yuwei Gao
Yiyu Lu
Songtao Yang
Xianzhu Xia
Publikationsdatum: 30.01.2016
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 5/2016
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-016-2763-5

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