Skip to main content
Erschienen in: Journal of Neuroinflammation 1/2017

Open Access 01.12.2017 | Research

αVβ3 Integrin regulates astrocyte reactivity

verfasst von: Raúl Lagos-Cabré, Alvaro Alvarez, Milene Kong, Francesca Burgos-Bravo, Areli Cárdenas, Edgardo Rojas-Mancilla, Ramón Pérez-Nuñez, Rodrigo Herrera-Molina, Fabiola Rojas, Pascal Schneider, Mario Herrera-Marschitz, Andrew F. G. Quest, Brigitte van Zundert, Lisette Leyton

Erschienen in: Journal of Neuroinflammation | Ausgabe 1/2017

Abstract

Background

Neuroinflammation involves cytokine release, astrocyte reactivity and migration. Neuronal Thy-1 promotes DITNC1 astrocyte migration by engaging αVβ3 Integrin and Syndecan-4. Primary astrocytes express low levels of these receptors and are unresponsive to Thy-1; thus, inflammation and astrocyte reactivity might be necessary for Thy-1-induced responses.

Methods

Wild-type rat astrocytes (TNF-activated) or from human SOD1G93A transgenic mice (a neurodegenerative disease model) were used to evaluate cell migration, Thy-1 receptor levels, signaling molecules, and reactivity markers.

Results

Thy-1 induced astrocyte migration only after TNF priming. Increased expression of αVβ3 Integrin, Syndecan-4, P2X7R, Pannexin-1, Connexin-43, GFAP, and iNOS were observed in TNF-treated astrocytes. Silencing of β3 Integrin prior to TNF treatment prevented Thy-1-induced migration, while β3 Integrin over-expression was sufficient to induce astrocyte reactivity and allow Thy-1-induced migration. Finally, hSOD1G93A astrocytes behave as TNF-treated astrocytes since they were reactive and responsive to Thy-1.

Conclusions

Therefore, inflammation induces expression of αVβ3 Integrin and other proteins, astrocyte reactivity, and Thy-1 responsiveness. Importantly, ectopic control of β3 Integrin levels modulates these responses regardless of inflammation.
Begleitmaterial
Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1186/​s12974-017-0968-5) contains supplementary material, which is available to authorized users.
Abkürzungen
[Ca2+]i
Intracellular calcium concentrations
ALS
Amyotrophic lateral sclerosis
ATP
Adenosine triphosphate
BBG
Brilliant Blue G
CNS
Central nervous system
FA
Focal adhesion
GFAP
Glial fibrillar acidic protein
P2X7R
P2X7 receptor
SOD1
Super oxide dismutase 1
TNF
Tumor necrosis factor

Background

Astrocytes are the main type of glial cell in the central nervous system (CNS). Their functions range from nursing neurons to forming the blood-brain barrier. Under pro-inflammatory conditions induced by injury or neurodegenerative disorders, such as Alzheimer’s disease and amyotrophic lateral sclerosis (ALS), astrocytes become reactive and take on a more stellate shape, while increasing in number and size. At the same time, the expression of several proteins is enhanced, including that of glial fibrillary acidic protein (GFAP) and inducible nitric oxide synthase (iNOS) [15]. This reactive process is referred to as astrogliosis [69]. Astrogliosis has been recognized as an inhibitory cue for axon regeneration. Nevertheless, it may have initial benefits for the injured area, since astrocytes restrict any damage to a small area, repair the blood brain barrier, and eliminate toxic factors from the surrounding area [810]. During astrogliosis, several molecules are released into the extracellular space, including ATP, adenosine, and a number of cytokines [8, 9]. One of the most important cytokines released to the injured area is tumor necrosis factor (TNF) [9, 11], which acts as an alert signal that induces reactivity of the neighboring astrocytes [12, 13].
Reactive astrocytes are a heterogeneous population of cells, and recent in vivo studies indicate that some astrocytes proliferate, while for another subset of labeled astrocytes hypertrophy and polarization, but not overt migration towards the site of injury are detected [1416]. However, several previously reported studies indicate that reactive astrocytes migrate to the damaged zone and form the glial scar, repairing the wounded area [6, 10, 17]. The protein osteopontin is among the various stimuli that induce cell migration; this requires interaction with αVβ3 Integrin [18, 19]. Importantly, reactive astrocytes have been shown to express increased levels of αVβ3 Integrin, the P2X7 receptor (P2X7R), and the hemichannels Connexin-43 and Pannexin-1 [4, 18, 20]. All these molecules are reportedly involved in astrocyte migration [21, 22].
The neuronal surface glycoprotein Thy-1 binds to astrocytes and thereby promotes adhesion and migration, processes that our group has studied in depth. Thy-1-induced adhesion and migration require interaction with the αVβ3 Integrin and Syndecan-4 receptors. These interactions trigger the recruitment of focal adhesion (FA) proteins and activate a signaling module, which in turn leads to the activation of the small GTPase RhoA and its effector ROCK and results in changes in the astrocyte actin cytoskeleton [2328]. The signaling cascade also includes the opening of the hemichannels Connexin-43 and Pannexin-1 and the release of ATP to the extracellular milieu, where it activates P2X7R, allowing calcium entry [21, 22]. After a more prolonged stimulation, Thy-1 induces the activation of FAK, PI3-kinase, and the GTPase Rac1, thus leading to astrocyte migration [26].
We have previously studied the Thy-1-induced signaling cascade using the DITNC1 astrocyte cell line. Considering that, in primary astrocytes, many of the molecules forming part of the Thy-1-induced signaling pathway are only present in low amounts [29, 30], we hypothesized that primary astrocytes would be refractory to Thy-1 stimulus. Additionally, many signaling proteins, including the Thy-1 receptors, are upregulated under pro-inflammatory conditions [3, 4, 18, 20, 30]. We therefore decided to directly study the effect of a pro-inflammatory environment on the response of primary astrocytes to Thy-1, and on the Thy-1-induced signaling cascade in these cells. This was achieved in two ways. Firstly, primary astrocytes were treated with the pro-inflammatory signaling factor TNF prior to Thy-1 application, comparing the results to those obtained from controls that had not been exposed to TNF. Secondly, astrocytes were used derived from an ALS model, a neurodegenerative disorder marked by neuroinflammation and the presence of reactive astrocytes [31].
ALS is a late-onset disease, which generally appears after 50 years of age. It is characterized by motor neuron dysfunction and degeneration and leads to death from respiratory failure [3234]. Astrocytes from ALS patient samples are activated [35] and show increased levels of Connexin-43 and GFAP [3, 36]. In murine ALS models, astrocytes are activated within a time frame before the onset of disease [31, 37]. Additionally, these reactive astrocytes are now recognized to assist ALS motor neuronal death by secreting inflammatory factors [33, 36, 3842]. To study the effect of Thy-1 signaling on astrocytes developing under the ALS neuroinflammatory conditions, the transgenic mouse carrying the human G93A mutated super oxide dismutase 1 (hSOD1G93A) was employed, the most widely used ALS model [1, 3, 33, 36, 39, 4143].
As discussed above, Thy-1-mediated astrocyte adhesion and migration relies on the αVβ3 Integrin receptor. Because β3 Integrin is upregulated in the presence of TNF in various cell types [18, 44, 45], it could be hypothesized that the effect of TNF on astrocyte reactivity is related to β3 Integrin expression. To further explore the role of this receptor molecule, Thy-1 signaling in primary astrocytes was therefore investigated, in which β3 Integrin expression had been either silenced or upregulated, both in the presence and absence of TNF.
Here, we show that primary astrocytes only responded to Thy-1 in a pro-inflammatory environment (+TNF), where proteins participating in the Thy-1 response were upregulated. In addition, neonatal primary astrocytes derived from a mouse model of ALS (hSOD1G93A) were found to express higher levels of reactivity markers and β3 Integrin, Syndecan-4, Connexin-43, and Pannexin-1 than those derived from wild-type non-transgenic littermates. The hSOD1G93A-derived astrocytes responded to Thy-1 without the need of cytokine addition. Importantly, in investigating the role of β3 Integrin in Thy-1-induced FA and astrocyte migration, over-expression of β3 Integrin was found to be sufficient to render primary astrocytes reactive and consequently responsive to Thy-1 stimulation in the absence of TNF. Conversely, silencing of β3 Integrin precluded the effect of Thy-1 on TNF-treated astrocytes. These results favor a working model in which inflammation enhances β3 Integrin expression, rendering astrocytes responsive to Thy-1 and thereby promoting their adhesion and migration, as required during the repair of the injured brain. Controlling β3 Integrin levels modulated astrocyte reactivity.

Methods

Animals

The care and use of rodents is detailed in protocols approved by the bioethical Committees of the Universidad de Chile and Universidad Andres Bello. Wistar neonatal rats (P1-P2) were obtained from the Universidad de Chile animal facility. Transgenic mice hemizygous for human SOD1G93A (hSOD1G93A) or non-transgenic mice (used as a control) were obtained from the Jackson Laboratory (Bar Harbor, USA). Transgenes were detected by PCR [39].

Primary cultures

Astrocytes were obtained from 1- to 2-day-old wild-type rats or non-transgenic and hSOD1G93A mice. Briefly, wild-type rats were sacrificed by decapitation. Brains were extracted and placed in ice-cold PBS under a microscope. Cortices were separated from brain and meninges and kept in fresh ice-cold PBS. To isolate the cells, cortices were treated with trypsin and mechanically disrupted. The resulting cell suspensions were passed through a 70-μm cell strainer, washed with DMEMF-12 (Gibco, Life Technologies, Grand Island, NY) supplemented with 10% FBS, and centrifuged at 1000 rpm. Following this, cells were counted and seeded at a density of 1.5 million cells per T-25 cell flask. The cell medium was changed every 48 h. Once confluent, neurons and other cell types were released to the supernatant by shaking in an orbital shaker at 180 rpm overnight. To induce a pro-inflammatory environment, rat primary astrocytes were stimulated with 10 ng/ml of TNF for 48 h. IL-1β, IL-6, or IFN-γ (R&D systems) were also used (kindly donated by Dr. Rommy von Bernhardi, P. Universidad Católica de Chile). Murine astrocyte cultures were prepared from the ventral spinal cord of P1-P2 hSOD1G93A transgenic mice, or from non-transgenic littermates as previously described [39, 41, 42]. Cultures were maintained in DMEM (Gibco, Life Technologies, Grand Island, NY) containing 10% FBS and 1% penicillin-streptomycin at 37 °C, 5% CO2. Cultures reached confluence after 2–3 weeks and contained > 95% GFAP+ astrocytes. Residual microglia were removed from both mouse and rat astrocytes by shaking cultures in an orbital shaker overnight (200 rpm).

Thy-1-Fc and Trail-R2-Fc preparation

Thy-1-Fc (wild-type and mutants) and Trail-R2-Fc fusion proteins were obtained as previously described [21, 26]. Prior to their use, Thy-1-Fc and Trail-R2-Fc were incubated with Protein A in a 10:1 ratio wile rotating gently on a shaker for 1 h at 4 °C. Trail-R2-Fc is a fusion protein of the receptor for the soluble apoptosis-inducing ligand Trail-R2, which is used as a control for possible non-specific effects caused by the Fc portion of the Thy-1 fusion protein [21]. Prior to each experiment involving Thy-1-Fc or Trail-R2-Fc treatment, astrocytes were serum-starved for at least 30 min in DMEM without serum.

Focal adhesion assay

Primary astrocytes were seeded on 12-mm coverslips and left to adhere for 24 h. Cells were then pre-treated with 10 ng/ml of TNF for 48 h to induce reactivity. Afterwards, astrocytes were rinsed with DMEM and left in serum-free medium for 30 min. This medium was then removed, and cells were incubated for 15 min with 4 μg of either Thy-1-Fc or Trail-R2-Fc coupled to Protein A in serum-free medium. FBS (3% for 5 min) was used as a positive control. Astrocytes were then washed and fixed for 10 min with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 for 10 min, and blocked in 2% bovine serum albumin (BSA)-PBS for 1 h. Cells were then incubated with anti-vinculin antibody (1:200, Sigma-Aldrich Co., Saint Louis, MO) for 1 h at 37 °C to stain for focal adhesions. Rhodamine-conjugated Phalloidin (Sigma-Aldrich Co., Saint Louis, MO) and DAPI (Sigma-Aldrich Co.) (0.025 μg/ml) were used to stain F-actin and nuclei, respectively. Quantification of focal adhesion area and number was performed as described previously [21, 26].

Transfection of primary astrocytes

Astrocytes were transfected with pEGFP-β3 Integrin (full length β3 Integrin subunit kindly donated by Dr. C. Rüegg, University of Friboug, Switzerland [46]) or a pool of three siRNAs against β3 Integrin (Ambion) using the Amaxa Nucleofector system following the manufacturer’s instructions for the VPI-1006 transfection kit and the program T-020 (Lonza, Cologne, Germany).

Wound-healing assay

Primary astrocytes were seeded in 24-well plates (100,000 cells per well). After 48 h of incubation in complete medium with or without TNF (10 ng/ml), two parallel wounds were introduced with a micropipette tip. Detached cells were washed away with serum-free medium, and after 30 min of starvation, cells were stimulated with 4 μg of Thy-1-Fc, Thy-1(RLE)-Fc, or Trail-R2-Fc (negative control) in serum-free medium for 24 h. All proteins were coupled to Protein A as indicated above. Alternatively, primary astrocytes were transfected with pEGFP-β3 Integrin or siRNA against β3 Integrin, prior to wounding cell monolayers and stimulating as above without TNF pre-treatment. Wound healing was evaluated based on images of the cell-free area at 0 and 24 h, as previously described [21, 26].

Western blot

Protein extracts were prepared in a lysis buffer (150 mM NaCl, 0.1% SDS, 0.25% sodium deoxycholate, 1% Triton-X100, in 50 mM Tris-HCl pH 7.4) supplemented with protease and phosphatase inhibitor cocktail (Biotool, Houston, TX). Extracts were electrophoretically separated on 10% SDS-PAGE gels and transferred to nitrocellulose (Millipore, Billerica, USA). The nitrocellulose was blocked with 5% w/v nonfat, dry milk in PBS containing 0.1% Tween-20 and subsequently incubated with the following primary antibodies: anti-β3 Integrin (1:2000, Millipore, Billerica, USA), anti-Syndecan-4 (1:2000, Abbexa, Cambridge, UK), anti-iNOS (Abbexa), anti-Connexin-43 (1:500; Santa Cruz Biotechnologies Dallas, TX), anti-Pannexin-1 (1:500; Santa Cruz Biotechnologies), anti-P2X7R (1:1000, Santa Cruz Biotechnologies), anti-GFAP (1:2000, Sigma-Aldrich Co.), and anti-misfolded human SOD1 (C4F6) (MediMabs, Quebec, Canada). The membrane was then washed and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5000; Abbexa, Cambridge, UK) or donkey anti-goat IgG (1:5000; Abbexa) for 1 h at room temperature. Bands were visualized with a chemiluminescence kit (Pierce, Thermo Scientific, Rockford, IL), according to the manufacturer’s instructions. We performed Western blot quantification by measuring band intensity in Photoshop 7 (Adobe) and normalizing to the corresponding loading control. To determine fold increase, all values were normalized to the average value obtained for control bands after any treatment.

Indirect immunofluorescence assay

Astrocytes were seeded on 12-mm coverslips and left to adhere for 24 h. Cells were then washed and fixed as indicated for the focal adhesion assay. Next, cells were stained with anti-Connexin-43 (1:200), anti-Pannexin-1 (1:200), anti-Syndecan-4 (1:200) or anti-P2X7R (1:200) antibodies followed by secondary antibodies (1:400) conjugated to IF488 or IF594 (Abbexa), and DAPI (0.025 μg/ml). Samples were analyzed using a confocal microscope C2+ (Nikon, Japan), with a 60×/1.40 objective and using the NIS-Elements software.

Extracellular ATP measurements

Twelve thousand primary astrocytes were seeded in 48-well plates. After 24 h, cells were incubated in serum-free media containing 100 μM of the exonuclease inhibitor ARL-67156 (Santa Cruz Biotechnologies) for 30 min at 37 °C. Following this, cells were stimulated with Thy-1-Fc:Protein A for 15 min. Where indicated, cells were incubated with a combination of Heptanol (Sigma-Aldrich Co.) (500 μM) and Probenecid (Sigma-Aldrich Co.) (1 mM). Next, culture medium was recovered and centrifuged for 5 min at 1000×g. The supernatants were incubated in the dark with 50 μl CellTiter-Glo® reaction mix (Promega, Madison, WI). Luminescence intensity was determined in a Synergy2 multi-mode reader (Biotek Instruments, Inc., Vermont), and the values were interpolated using a calibration curve obtained from different ATP concentrations (0.01, 0.1, 1, and 10 μM).

Measurement of intracellular calcium kinetics

Primary astrocytes were seeded on 25-mm coverslips (400,000 cells) and left to adhere for 24 h. The cells were then incubated with 5 μM Fluo-4-AM (Life Technologies, Grand Island, NY) in Ringer solution at 37 °C for 30 min, after which they were washed and left in the same solution. Following this, astrocytes were treated with the hemichannel blockers Heptanol (500 μM) and Probenecid (1 mM) for 30 min. Alternatively, they were treated with the P2X7R blocker BBG (American Bioanalytical, Natick, MA) (5 μM for 1 h). Images were acquired at 2-s intervals with an XM10 camera (Olympus Corp.). Thy-1-Fc or Trail-R2-Fc, both coupled to Protein A, were added 120 s after initiating acquisition. Fluorescence intensity was quantified in 100 cells per condition using ImageJ. The results were expressed as (F-F0)/F0, where F is the change in fluorescence and F0 is the basal fluorescence. To measure calcium in astrocytes transfected with pEGFP-β3 Integrin, cells were loaded with the red probe Cal-590 AM (AAT Bioquest) at 4 μM following the manufacturers’ protocol.

Statistical analyses

Results are shown as the means ± standard error of the mean (s.e.m.) for n = 3 or more experiments. The results were analyzed using Kruskall-Wallis test and Dunn’s post-test. Statistical significance was set at p < 0.05.

Results

A pro-inflammatory stimulus endows primary astrocytes with Thy-1 responsive properties

Thy-1 has been shown to induce of DITNC1 astrocyte migration by engaging αVβ3 Integrin and Syndecan-4 [26] and triggering signaling pathways downstream of these receptors [21]. Here, the effect of Thy-1 on wild-type primary astrocytes was tested. To this end, rat astrocytes were isolated from neonatal brains. Primary cultures were prepared for FA and migration assays and starved for 30 min prior to the treatment with Thy-1-Fc, serum (FBS, positive control), or Trail-R2-Fc (negative control) [21, 26]. Cells forming FAs were monitored by vinculin staining, whereas cell migration was evaluated in a wound-healing assay. We found that, unlike FBS-treated astrocytes, the stimulation with Thy-1-Fc did not lead to FA formation and the cells were morphologically similar to non-stimulated control cells (Fig. 1a).
Because only reactive astrocytes migrate after damage to the CNS [2], primary astrocytes were thought to require activation to respond to Thy-1 stimulation. Thus, astrocytes were treated with TNF to emulate a pro-inflammatory environment and tested for the astrocyte reactivity markers GFAP and iNOS. Levels of these proteins were increased by TNF treatment (Additional file 1: Figure S1A). Astrocyte morphology also changed (Additional file 1: Figure S1B), indicating the presence of reactive astrocytes in these TNF-treated cultures.
Importantly, TNF-treated astrocytes became responsive to the addition of Thy-1-Fc, which induced formation of FAs and stress fibers (Fig. 1a). Our results show that Thy-1 stimulated only those cells previously treated with TNF (Fig. 1b) or other pro-inflammatory cytokines (Additional file 1: Figure S1C), while stimulation with serum induced FA formation in cells with or without TNF pre-treatment (Fig. 1a, b). The effect of Thy-1-Fc on astrocyte migration was also tested for these astrocytes. As expected, primary astrocytes migrated upon Thy-1 stimulation only when cells were previously treated with TNF (Fig. 1c, d). As shown for FA, serum-treated cells responded independently of TNF treatment (Fig. 1c, d). These results show that primary astrocytes assembled FA and migrated in response to FBS, but that they were insensitive to Thy-1 unless previously activated with TNF or other pro-inflammatory cytokines.

TNF pre-treatment enables Thy-1 signaling in astrocytes

Thy-1 binding to αVβ3 Integrin in DITNC1 astrocytes has been recently demonstrated to be followed by intracellular signaling events that include opening of the hemichannels Pannexin-1 and Connexin-43 and release of ATP to the extracellular milieu [21]. The ATP activates P2X7R, allowing Ca2+ entry and PKCα activation, followed by Syndecan-4-triggered signaling pathways [22]. The presence of these receptors as well as the activation of similar signaling pathways in wild-type rat primary astrocytes treated with TNF and stimulated by Thy-1 was therefore explored.
We found that pretreatment with TNF increased the levels of β3 Integrin, Syndecan-4, P2X7R, Connexin-43, and Pannexin-1 (Fig. 2a). β3 Integrin levels were also enhanced by other pro-inflammatory cytokines (Additional file 2: Figure S2). Next, the subcellular localization of these proteins was evaluated. Connexin-43 was located mainly in vesicles near the nucleus in untreated primary astrocytes but was redistributed to the cell periphery upon TNF treatment (Fig. 2b). Notably, this staining pattern was also observed in isolated cells, indicative of hemichannel rather than gap junction configuration for Connexin-43. Pannexin-1 was also redistributed; after TNF treatment, it appeared at the tip of cellular protrusions (Fig. 2b). The intensity of Syndecan-4 and P2X7R fluorescent signals increased, but neither receptor showed changes in localization (Fig. 2b).
Since hemichannel protein levels increased following TNF treatment, and because ATP is released by hemichannel opening upon Thy-1 stimulation [21, 22], ATP release to the extracellular medium in cells treated with TNF followed by Thy-1 stimulation was measured. ATP was released only when cells were pre-treated with TNF. The hemichannel blockers Probenecid and Heptanol reduced ATP release to control levels (Fig. 2c). Because extracellular ATP activates the purinergic receptor P2X7, intracellular calcium levels ([Ca2+]i) were measured following Thy-1 stimulation with and without TNF treatment. Intracellular calcium concentrations were increased by Thy-1 addition (to a maximum value of 1.61 ± 0.27) only in astrocytes pre-treated with TNF (Fig. 2d). In addition, blocking the hemichannels with a mixture of Probenecid and Heptanol or inhibiting P2X7R with Brilliant Blue G (BBG) abolished most of the [Ca2+]i increase induced by Thy-1 stimulation, with maximum values reaching 1.01 ± 0.02 and 1.08 ± 0.09, respectively (Fig. 2d). Of note, TNF treatment per se showed no effect on ATP release or [Ca2+]i as observed in cells treated with TNF and Trail-R2-Fc compared to those treated only with Trail-R2-Fc (Fig. 2c, d).
These results indicate that hemichannel opening, ATP release, P2X7R activation, and Ca2+ uptake are important steps in the signaling pathway triggered by Thy-1 in reactive primary astrocytes. In line with this, the ATP hydrolyzing enzyme Apyrase and the P2X7R inhibitor BBG both precluded Thy-1-induced migration of activated astrocytes (Fig. 2e). Therefore, and as previously reported for DITNC1 astrocytes [21, 22], these results demonstrate that ATP release and Ca2+ uptake via P2X7R are fundamental to the Thy-1-induced migration of astrocytes.

Over-expression of β3 Integrin is sufficient to allow Thy-1-induced migration in non-activated astrocytes

Since β3 Integrin expression was enhanced after TNF treatment in primary astrocytes (Fig. 2a), and because αVβ3 Integrin is a receptor for Thy-1 [2126, 28, 4749], the role of this Integrin in Thy-1-induced astrocyte migration was further explored. To this end, astrocytes transfected with siRNA targeting β3 Integrin were treated with TNF. β3 Integrin-silenced cells were found to contain low levels of β3 Integrin (not shown). These levels were kept low even after TNF treatment, in comparison to TNF-treated cells transfected with the control siRNA (Fig. 3a). The β3 Integrin role in Thy-1-induced migration was then evaluated in a wound-healing assay. Thy-1-induced migration was diminished in β3 Integrin-silenced cells compared with TNF-treated cells transfected with the control siRNA (Fig. 3a). The negative control Trail-R2-Fc had no effect on astrocyte migration (Fig. 3a).
Considering that TNF treatment induced the upregulation of many surface proteins, and that β3 Integrin silencing was enough to prevent Thy-1-induced migration, the effect of the β3 Integrin over-expression in the absence of TNF treatment was evaluated. Successful transfection of astrocytes with a vector containing pEGFP-β3 Integrin was corroborated by evaluating β3 Integrin levels with immunoblotting (Fig. 3b). Using a wound-healing assay, the β3 Integrin over-expression was observed to render astrocytes responsive to Thy-1, which promoted wound closure even in the absence of pro-inflammatory stimuli (Fig. 3b). Notably, β3 Integrin over-expression led to increased levels for many other key components of the Thy-1 response, including Syndecan-4, P2X7R, Connexin-43, and Pannexin-1 (Fig. 3c). Together, these results indicate that β3 Integrin over-expression is sufficient to permit Thy-1-induced migration of primary astrocytes, without the requirement for TNF treatment, and that this effect might be due to a role for β3 Integrin in controlling the expression of other key components of the Thy-1 signaling pathway.
To confirm that the effect of Thy-1 on wild-type primary astrocytes is mediated through the interaction of Thy-1 and αVβ3 Integrin, a mutant Thy-1 carrying an amino acid substitution in the αVβ3 Integrin-binding domain was used to stimulate TNF-treated astrocytes. Impaired functionality of this mutated protein has been previously reported [21, 26]. Our results show that, in contrast to wild-type Thy-1, mutant Thy-1 did not induce astrocyte migration (Fig. 3d) confirming the importance of Thy-1/αVβ3 Integrin interaction in the reported effects.
Having confirmed β3 Integrin as a crucial mediator of Thy-1-triggered adhesion and migration, processes shown here to be limited to reactive astrocytes, and considering its upregulation in the presence of TNF, we were curious to explore whether the over-expression of this integrin had any general effects on astrocyte reactivity. The levels of the known markers of astrocyte reactivity iNOS and GFAP in pEGFP-β3 Integrin transfected astrocytes by western blotting were therefore evaluated. Importantly, β3 Integrin over-expression, but not EGFP used as a control, was found to increase the expression of these molecules (Fig. 3e), suggesting that the sole enhanced expression of this integrin was sufficient for these astrocytes to show properties of reactivity. Moreover, Thy-1 engagement of overexpressed β3 Integrin in astrocytes increased intracellular [Ca2+] and ATP levels (Additional file 3: Figures S3A and S3B). Taken together, these observations indicate that β3 integrin represents a key molecule in the Thy-1-induced signaling.
Importantly, these results uncover a critical role for β3 Integrin in primary astrocyte activation in a pro-inflammatory environment, a striking and unexpected function for this cell adhesion molecule in astrocytes.

Thy-1 responsiveness increases in astrocytes derived from transgenic hSOD1G93A mice

Neuroinflammatory diseases, such as ALS, involve cytokine release from various glial cells in the CNS [11]. Both in ALS patients and murine models of the disease, astrocytes become reactive in the spinal cord, lateral corticospinal tracts and in different brain regions before the appearance of motor symptoms [31, 50, 51]. It is therefore of interest to study the Thy-1 responsiveness of astrocytes derived from a murine model of ALS. Here, astrocytes from hSOD1G93A mice and from their non-transgenic littermates were used [1, 3, 36, 4143].
The activation state of these ALS astrocytes was first assessed by measuring the levels of the reactivity markers GFAP and iNOS by western blotting. The levels of both proteins were found increased when compared to astrocytes from non-transgenic animals (Additional file 4: Figure S4). In addition, vinculin and F-actin staining of non-transgenic and hSOD1G93A-derived astrocytes revealed differences in their FAs and actin cytoskeleton. Astrocytes from non-transgenic mice were round in shape with low levels of FAs, while hSOD1G93A-derived astrocytes were polygonal with better-defined actin stress fibers and fewer focal contacts (Fig. 4a, upper panels). Following Thy-1 addition, astrocytes from non-transgenic mice did not change, while hSOD1G93A-derived astrocytes showed an increased number of FAs and robust stress fibers (Fig. 4a, bottom panels). Next, migration was evaluated in a wound-healing assay. Only hSOD1G93A-derived astrocytes were found to have migrated after Thy-1-Fc stimulation (Fig. 4b). As expected, the addition of Thy-1(RLE)-Fc, which lacks the β3 Integrin-binding domain, had no effect on the migration of hSOD1G93A-derived astrocytes (Fig. 4b). These results confirm that hSOD1G93A-derived astrocytes, like TNF-activated wild-type primary astrocytes (see Fig. 1), respond to Thy-1 through αVβ3 Integrin interaction, thereby increasing cell adhesion and migration.
Because increased levels of αVβ3 Integrin, Syndecan-4, Connexin-43, Pannexin-1, and P2X7R had been detected in TNF-treated rat wild-type primary astrocytes, hSOD1G93A-derived astrocytes were tested for similar increases in the levels of these proteins. Except for P2X7R, all of these proteins were found to be upregulated in these cells (Fig. 4c).
Together, these results confirm that hSOD1G93A-derived astrocytes are reactive, show increased expression of surface proteins involved in Thy-1-induced signaling, and enhanced adhesion and migration after Thy-1-Fc stimulation. It is intriguing that, despite their early postnatal origin (P1-P2 stages), hSOD1G93A-derived astrocytes showed properties of reactive astrocytes after only 4 weeks in culture, suggesting that despite their early origin, these astrocytes were already primed to become reactive.

Discussion

Inflammatory cytokines are widely considered causative in triggering astrocyte reactivity [52]. Here, we show that wild-type primary astrocytes respond to Thy-1 by adhering and migrating only under inflammatory conditions (+TNF). We also show that in primary astrocytes, Thy-1 induces similar signaling pathways to those reported for DITNC1 astrocytes, namely, hemichannel opening, ATP release, activation of P2X7R, Ca2+ entry, early cell adhesion, and finally, cell polarization and migration [21, 26, 53, 54]. In addition, hSOD1G93A-derived astrocytes respond to Thy-1 without any requirement for additional cytokines. Of note, ectopically expressed β3 Integrin increases the levels of other important players of the Thy-1-induced signaling pathways, including Syndecan-4 and the Connexin-43/Pannexin-1 hemichannels, as well as markers of reactive astrocytes, such as iNOS and GFAP. Furthermore, over-expression of β3 Integrin is sufficient to render astrocytes responsive to Thy-1. Therefore, we propose that β3 Integrin plays a preponderant role in determining Thy-1-induced effects in astrocytes and possibly also in controlling astrocyte reactivity.
After injury to the CNS, microglia generate a pro-inflammatory environment by liberating TNF [11]. This has been shown to enhance the expression of integrins [55] and cell adhesion molecules, such as ICAM-1, VCAM-1, and E-selectin [52, 56, 57]. In our studies, TNF induced astrocyte reactivity, increasing the expression levels of reported markers of astrocytosis as well as the Thy-1 receptors αVβ3 Integrin and Syndecan-4 [25, 28, 58]. Thy-1 receptor upregulation enables cells to respond to the ligand and thereby initiate astrocyte adhesion and migration. These findings suggest that a pro-inflammatory environment is required for astrocytes to become responsive to neuronal signals such as Thy-1. The low levels of β3 Integrin observed in primary astrocytes may explain the unresponsiveness of these cells to Thy-1. In this context, it is of interest that TNF also induces αVβ3 Integrin expression, along with a αVβ3 Integrin-dependent cell migration, in endothelial cells [44]; this indicates that increased αVβ3 Integrin expression is relevant to migration under pro-inflammatory conditions in other cellular models.
In the present study, the application of TNF to primary astrocytes leads to an increased expression of αVβ3 Integrin, Syndecan-4, P2X7R, Connexin-43, and Pannexin-1. All of these molecules have previously been shown to be required for Thy-1-induced cell migration [21]. Others have reported that Connexin-43 hemichannnels are activated by treating astrocytes with a mixture of TNF and IL-1β. This leads to neuronal death, an effect not observed in astrocytes lacking Connexin-43 [59]. Moreover, prenatal exposure to LPS, a well-known pro-inflammatory agent, induces activation of astrocytes with increased levels of Connexin-43 and Pannexin-1 [4]. Additionally, treating astrocytes with TNF for only 3 h induces expression of Connexin-43 hemichannels [60]. These results suggest that primary astrocytes exposed to a pro-inflammatory environment upregulate surface receptors involved in the Thy-1-Integrin-induced signaling pathways.
ATP release from hemichannels activated P2X7R and thus increased intracellular calcium, only after TNF treatment. ATP release and P2X7R activation has been previously shown to be involved in Thy-1-induced astrocyte responses, first inducing cell adhesion [22] and subsequently migration [21]. Of note, increased levels of ATP have been associated with brain damage in humans. Patients with radiation-induced brain injury show high levels of extracellular ATP, IL-6, and TNF in cephalorachidian fluid [61]. In addition, exposure of primary astroglial cultures to radiation increases extracellular levels of ATP, IL-6, and TNF, unless P2X7R is inhibited or silenced [61]. This suggests an important role for ATP and P2X7R in the activation of glial cultures and progression of brain injuries. Recently, increased expression of P2X7R and Connexin-43 has been linked to astrogliosis in autoimmune encephalomyelitis, where the increase of P2X7R coincided with the appearance of astrocyte reactivity markers, such as GFAP and S100β. The addition of P2X7R blockers led to a reduction in astrogliosis markers [20], suggesting that the increase in P2X7R is involved in astrocyte reactivity. In line with this, published evidence indicates that P2X7R over-expression leads to microglia activation [62, 63]. In the present study, P2X7R protein levels increased after TNF treatment, while the inhibition of P2X7R and the apyrase-catalyzed reduction of extracellular ATP both decreased Thy-1-induced astrocyte migration. However, it remains to be determined whether P2X7R is a direct or an indirect target of TNF.
The increase in [Ca2+]i induced by Thy-1 stimulus is necessary for astrocyte migration. Ca2+ influx and Ca2+ release from intracellular stores via stimulation of IP3R participate in Thy-1-induced astrocyte migration, since P2X7R silencing as well as the addition of IP3R inhibitors prevent the migration of astrocytes with a reactive phenotype [21]. In addition, the calcium-chelator BAPTA-AM blocks Thy-1-induced hemichannel opening and ATP release, two key steps in the process of Thy-1-induced DITNC1 astrocyte migration [21]. In agreement with our observations, Fang and coworkers published that Orexin, a neuropeptide that evokes responses similar to those triggered by Thy-1 in astrocytes, induces migration in rat astrocytes in a Ca2+-dependent manner, which can be blocked by either the addition of BAPTA-AM or an IP3R blocker, such as 2-APB [64], supporting the notion that increased [Ca2+]i is necessary for astrocyte migration.
In primary astrocytes, TNF treatment is required for the Thy-1 response; however, TNF by itself neither induces migration nor increases Ca2+ levels (Figs. 1c, d and 2d), but rather promotes changes in protein expression to generate a migratory phenotype responsive to Thy-1. Of note, αVβ3 Integrin, Syndecan-4, and P2X7 receptors trigger parallel and different signaling pathways [21]; thus, although elevated [Ca2+]i is required for Thy-1-stimulated astrocyte migration, it is not expected to be sufficient alone to induce the migratory phenotype, which results from more complex and interconnected signaling cascades.
Thy-1 elicits responses by engaging its receptors αVβ3 Integrin and Syndecan-4 [21, 22, 26]. Interestingly, β3 Integrin over-expression was sufficient to enable primary astrocytes to respond to Thy-1, thereby showing an effect similar to that of TNF treatment. Others have reported an increased cell migration, invasion and tumorigenesis of cancer cells over-expressing β3 Integrin following stimulation with osteopontin [65], an extracellular matrix protein, which is upregulated and secreted after TNF treatment, and which induces directional astrocyte migration in an αVβ3 Integrin-dependent manner [18, 19]. This is in line with our findings, since it indicates that increased levels of β3 Integrin protein make cells more responsive to external cues. However, over-expression of β3 Integrin alone does not induce tumorigenesis in the absence of osteopontin [65]. This is paralleled by our own observation that in the absence of Thy-1, migration was not increased in β3 Integrin over-expressing astrocytes, confirming that elevated levels of β3 Integrin render astrocytes sensitive to the neuronal Thy-1, rather than increasing cell autonomous migration. The intriguing possibility that increased αVβ3 Integrin expression might guide reactive astrocytes to regions rich in “damage” signals, allowing them to interact with neuronal Thy-1 in the damaged zone, awaits further studies.
Furthermore, β3 Integrin appears to control the expression of other proteins involved in the Thy-1 response. It has been reported that αVβ3 Integrin acts upstream of NF-κB in several cell types [6668] and that tyrosine 759 of β3 Integrin is required for NF-κB activation [68], suggesting that this pathway, or a similar one, might be activated upon over-expression of β3 Integrin. Like αVβ3 Integrin, the expression of the Thy-1 co-receptor, Syndecan-4, is also increased in an NF-κB-dependent manner when EAhy926 cells are treated with TNF [69]. It is possible that NF-κB activation might increase expression of the surface proteins reported in the present study in response to TNF. In hepatocytes from acute-on-chronic liver failure, increased levels of NF-κB are associated with increased expression of Connexin-43, but not Connexin-26 or Connexin-32 [70]. In isolated aorta sections, Connexin-43 levels are increased downstream of NF-κB and ERK1/2 activation [71]. Additionally, IL-8 regulates αVβ3 Integrin expression in a PI3K/Akt/NF-κB-dependent manner in MDA-MB-231 cancer cells [45]. Similarly, when glioblastoma multiforme cells are treated with the phorbol ester PMA, NF-κB induces the expression of αVβ3 Integrin along with fibronectin and vitronectin [72]. This suggests a possible role for NF-κB in upregulating the expression of components in the Thy-1 activated pathway. Consequently, β3 Integrin could be placed upstream or downstream of NF-κB. Considering that β3 Integrin over-expression is sufficient to increase the expression of reactive astrocyte markers and to render astrocytes sensitive to Thy-1 stimulation in the absence of TNF, and since β3 Integrin silencing precludes the effect of Thy-1 in TNF-treated cells, we propose that β3 Integrin expression can control astrocyte reactivity. Whether it does so by activating NF-κB remains to be assessed.
To place our findings into a patho-physiological context, astrocytes were analyzed from the hSOD1G93A ALS mouse model. In this model, astrocytes are reported to be reactive before the onset motor symptoms, and the degree of reactivity correlates with the neurodegenerative process [50]. Elevated expression levels of GFAP, Vimentin, and CD44 in tissue sections from post-mortem spinal cord in ALS patients also indicate astrocyte reactivity [36]. Here, Thy-1 is shown to induce adhesion and migration of hSOD1G93A-derived astrocytes without the need of prior TNF treatment. As might be expected, Connexin-43 levels substantially increased in these cells (Fig. 4c; see also [3]). For P2X7R, modest levels of expression are associated with increased ALS severity, as well as increased astrocyte and microglia reactivity [1]. Consistent with this, we observed low levels of P2X7R in hSOD1G93A astrocytes, suggesting that these levels are sufficient for Thy-1-induced migration. Therefore, astrocytes derived from hSOD1G93A mice are reactive and responsive to Thy-1. Notably, unlike P2X7R, Pannexin-1, Syndecan-4, and αVβ3 Integrin expression levels were increased. Such alterations have not been associated with ALS to date, and further studies are required to clarify the specific contribution of each of these proteins.

Conclusions

We show for the first time that primary astrocytes must be primed by exposure to a pro-inflammatory environment before they can change their shape, adhesion, and migratory properties in response to neuronal Thy-1. β3 Integrin was identified as a fundamental mediator of Thy-1-induced migration, not only as a receptor, but also as a regulator of the signaling pathway downstream of the Thy-1 receptors. In addition, we propose β3 Integrin as a key target to control astrocyte reactivity. Finally, a novel association of Pannexin-1, Syndecan-4, and β3 Integrin with ALS is here uncovered. Therefore, our results support Thy-1 and β3 Integrin/Syndecan-4 as potential targets for the clinical management of neurodegenerative diseases involving inflammatory processes.

Acknowledgements

Not applicable.

Funding

This study was supported by the following grants: FONDECYT #3140460 (RLC), #1150744 (LL), #1130250 (AFGQ), #1170925 (AFGQ), #1140301 (BvZ), #3140471 (AC); #3170645 (RP-N); CONICYT #21090323 (AA), and #21130008 (FBB); CONICYT-FONDAP #15130011 (AFGQ); Swiss National Science Foundation #310030_156961 (PS); Anillo ACT-1114, ATA 2014-F-034 and DRI USA 2013-0030 (BvZ).

Availability of data and materials

All data generated or analyzed as part of this study are included in this article and its supplementary information files.
The bioethics committees of Universidad de Chile and Universidad Andres Bello approved the bioethical protocols for animal care and handling.
Not applicable.

Competing interests

The authors declare that they have no competing interests.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://​creativecommons.​org/​licenses/​by/​4.​0/​), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.
Anhänge

Additional files

Literatur
1.
Zurück zum Zitat Apolloni S, et al. Ablation of P2X7 receptor exacerbates gliosis and motoneuron death in the SOD1-G93A mouse model of amyotrophic lateral sclerosis. Hum Mol Genet. 2013;22(20):4102–16.CrossRefPubMed Apolloni S, et al. Ablation of P2X7 receptor exacerbates gliosis and motoneuron death in the SOD1-G93A mouse model of amyotrophic lateral sclerosis. Hum Mol Genet. 2013;22(20):4102–16.CrossRefPubMed
2.
Zurück zum Zitat Villarreal A, et al. Isolation and characterization of ischemia-derived astrocytes (IDAs) with ability to transactivate quiescent astrocytes. Front Cell Neurosci. 2016;10:139.CrossRefPubMedPubMedCentral Villarreal A, et al. Isolation and characterization of ischemia-derived astrocytes (IDAs) with ability to transactivate quiescent astrocytes. Front Cell Neurosci. 2016;10:139.CrossRefPubMedPubMedCentral
3.
Zurück zum Zitat Almad AA, et al. Connexin 43 in astrocytes contributes to motor neuron toxicity in amyotrophic lateral sclerosis. Glia. 2016;64(7):1154–69.CrossRefPubMed Almad AA, et al. Connexin 43 in astrocytes contributes to motor neuron toxicity in amyotrophic lateral sclerosis. Glia. 2016;64(7):1154–69.CrossRefPubMed
4.
Zurück zum Zitat Avendano BC, et al. Prenatal exposure to inflammatory conditions increases Cx43 and Panx1 unopposed channel opening and activation of astrocytes in the offspring effect on neuronal survival. Glia. 2015;63:2058–72. Avendano BC, et al. Prenatal exposure to inflammatory conditions increases Cx43 and Panx1 unopposed channel opening and activation of astrocytes in the offspring effect on neuronal survival. Glia. 2015;63:2058–72.
5.
Zurück zum Zitat Hung CC, et al. Astrocytic GAP43 induced by the TLR4/NF-kappaB/STAT3 axis attenuates astrogliosis-mediated microglial activation and neurotoxicity. J Neurosci. 2016;36(6):2027–43.CrossRefPubMed Hung CC, et al. Astrocytic GAP43 induced by the TLR4/NF-kappaB/STAT3 axis attenuates astrogliosis-mediated microglial activation and neurotoxicity. J Neurosci. 2016;36(6):2027–43.CrossRefPubMed
6.
Zurück zum Zitat Sofroniew MV. Reactive astrocytes in neural repair and protection. Neuroscientist. 2005;11(5):400–7.CrossRefPubMed Sofroniew MV. Reactive astrocytes in neural repair and protection. Neuroscientist. 2005;11(5):400–7.CrossRefPubMed
7.
Zurück zum Zitat Osborn LM, et al. Astrogliosis: An integral player in the pathogenesis of Alzheimer’s disease. Prog Neurobiol. 2016;144:121–41.CrossRefPubMed Osborn LM, et al. Astrogliosis: An integral player in the pathogenesis of Alzheimer’s disease. Prog Neurobiol. 2016;144:121–41.CrossRefPubMed
8.
Zurück zum Zitat Rolls A, Shechter R, Schwartz M. The bright side of the glial scar in CNS repair. Nat Rev Neurosci. 2009;10(3):235–41.CrossRefPubMed Rolls A, Shechter R, Schwartz M. The bright side of the glial scar in CNS repair. Nat Rev Neurosci. 2009;10(3):235–41.CrossRefPubMed
9.
Zurück zum Zitat Buffo A, Rolando C, Ceruti S. Astrocytes in the damaged brain: molecular and cellular insights into their reactive response and healing potential. Biochem Pharmacol. 2010;79(2):77–89.CrossRefPubMed Buffo A, Rolando C, Ceruti S. Astrocytes in the damaged brain: molecular and cellular insights into their reactive response and healing potential. Biochem Pharmacol. 2010;79(2):77–89.CrossRefPubMed
10.
Zurück zum Zitat Faulkner JR, et al. Reactive astrocytes protect tissue and preserve function after spinal cord injury. J Neurosci. 2004;24(9):2143–55.CrossRefPubMed Faulkner JR, et al. Reactive astrocytes protect tissue and preserve function after spinal cord injury. J Neurosci. 2004;24(9):2143–55.CrossRefPubMed
11.
Zurück zum Zitat Welser-Alves JV, Milner R. Microglia are the major source of TNF-alpha and TGF-beta1 in postnatal glial cultures; regulation by cytokines, lipopolysaccharide, and vitronectin. Neurochem Int. 2013;63(1):47–53.CrossRefPubMed Welser-Alves JV, Milner R. Microglia are the major source of TNF-alpha and TGF-beta1 in postnatal glial cultures; regulation by cytokines, lipopolysaccharide, and vitronectin. Neurochem Int. 2013;63(1):47–53.CrossRefPubMed
12.
Zurück zum Zitat Balasingam V, et al. Reactive astrogliosis in the neonatal mouse brain and its modulation by cytokines. J Neurosci. 1994;14(2):846–56.PubMed Balasingam V, et al. Reactive astrogliosis in the neonatal mouse brain and its modulation by cytokines. J Neurosci. 1994;14(2):846–56.PubMed
13.
Zurück zum Zitat Hennessy E, Griffin EW, Cunningham C. Astrocytes are primed by chronic neurodegeneration to produce exaggerated chemokine and cell infiltration responses to acute stimulation with the cytokines IL-1beta and TNF-alpha. J Neurosci. 2015;35(22):8411–22.CrossRefPubMedPubMedCentral Hennessy E, Griffin EW, Cunningham C. Astrocytes are primed by chronic neurodegeneration to produce exaggerated chemokine and cell infiltration responses to acute stimulation with the cytokines IL-1beta and TNF-alpha. J Neurosci. 2015;35(22):8411–22.CrossRefPubMedPubMedCentral
14.
Zurück zum Zitat Bardehle S, et al. Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation. Nat Neurosci. 2013;16(5):580–6.CrossRefPubMed Bardehle S, et al. Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation. Nat Neurosci. 2013;16(5):580–6.CrossRefPubMed
16.
Zurück zum Zitat Sirko S, et al. Reactive glia in the injured brain acquire stem cell properties in response to sonic hedgehog. [corrected]. Cell Stem Cell. 2013;12(4):426–39.CrossRefPubMed Sirko S, et al. Reactive glia in the injured brain acquire stem cell properties in response to sonic hedgehog. [corrected]. Cell Stem Cell. 2013;12(4):426–39.CrossRefPubMed
17.
Zurück zum Zitat Bush TG, et al. Leukocyte infiltration, neuronal degeneration, and neurite outgrowth after ablation of scar-forming, reactive astrocytes in adult transgenic mice. Neuron. 1999;23(2):297–308.CrossRefPubMed Bush TG, et al. Leukocyte infiltration, neuronal degeneration, and neurite outgrowth after ablation of scar-forming, reactive astrocytes in adult transgenic mice. Neuron. 1999;23(2):297–308.CrossRefPubMed
18.
Zurück zum Zitat Ellison JA, Barone FC, Feuerstein GZ. Matrix remodeling after stroke. De novo expression of matrix proteins and integrin receptors. Ann N Y Acad Sci. 1999;890:204–22.CrossRefPubMed Ellison JA, Barone FC, Feuerstein GZ. Matrix remodeling after stroke. De novo expression of matrix proteins and integrin receptors. Ann N Y Acad Sci. 1999;890:204–22.CrossRefPubMed
19.
Zurück zum Zitat Wang X, et al. Delayed expression of osteopontin after focal stroke in the rat. J Neurosci. 1998;18(6):2075–83.PubMed Wang X, et al. Delayed expression of osteopontin after focal stroke in the rat. J Neurosci. 1998;18(6):2075–83.PubMed
20.
Zurück zum Zitat Grygorowicz T, Welniak-Kaminska M, Struzynska L. Early P2X7R-related astrogliosis in autoimmune encephalomyelitis. Mol Cell Neurosci. 2016;74:1–9.CrossRefPubMed Grygorowicz T, Welniak-Kaminska M, Struzynska L. Early P2X7R-related astrogliosis in autoimmune encephalomyelitis. Mol Cell Neurosci. 2016;74:1–9.CrossRefPubMed
21.
Zurück zum Zitat Alvarez A, et al. Integrin-mediated transactivation of P2X7R via hemichannel-dependent ATP release stimulates astrocyte migration. Biochim Biophys Acta. 2016;1863(9):2175–88.CrossRefPubMed Alvarez A, et al. Integrin-mediated transactivation of P2X7R via hemichannel-dependent ATP release stimulates astrocyte migration. Biochim Biophys Acta. 2016;1863(9):2175–88.CrossRefPubMed
22.
Zurück zum Zitat Henriquez M, et al. ATP release due to Thy-1-integrin binding induces P2X7-mediated calcium entry required for focal adhesion formation. J Cell Sci. 2011;124(Pt 9):1581–8.CrossRefPubMedPubMedCentral Henriquez M, et al. ATP release due to Thy-1-integrin binding induces P2X7-mediated calcium entry required for focal adhesion formation. J Cell Sci. 2011;124(Pt 9):1581–8.CrossRefPubMedPubMedCentral
23.
Zurück zum Zitat Avalos AM, et al. Aggregation of integrins and RhoA activation are required for Thy-1-induced morphological changes in astrocytes. J Biol Chem. 2004;279(37):39139–45.CrossRefPubMed Avalos AM, et al. Aggregation of integrins and RhoA activation are required for Thy-1-induced morphological changes in astrocytes. J Biol Chem. 2004;279(37):39139–45.CrossRefPubMed
24.
Zurück zum Zitat Avalos AM, et al. Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function. Biol Res. 2002;35(2):231–8.CrossRefPubMed Avalos AM, et al. Signaling triggered by Thy-1 interaction with beta 3 integrin on astrocytes is an essential step towards unraveling neuronal Thy-1 function. Biol Res. 2002;35(2):231–8.CrossRefPubMed
25.
Zurück zum Zitat Avalos AM, et al. Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alphavbeta3 integrin that activates PKCalpha and RhoA. J Cell Sci. 2009;122(Pt 19):3462–71.CrossRefPubMedPubMedCentral Avalos AM, et al. Neuronal Thy-1 induces astrocyte adhesion by engaging syndecan-4 in a cooperative interaction with alphavbeta3 integrin that activates PKCalpha and RhoA. J Cell Sci. 2009;122(Pt 19):3462–71.CrossRefPubMedPubMedCentral
26.
Zurück zum Zitat Kong M, et al. Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of alphaVbeta3 integrin and syndecan-4. Biochim Biophys Acta. 2013;1833(6):1409–20.CrossRefPubMedPubMedCentral Kong M, et al. Thy-1-mediated cell-cell contact induces astrocyte migration through the engagement of alphaVbeta3 integrin and syndecan-4. Biochim Biophys Acta. 2013;1833(6):1409–20.CrossRefPubMedPubMedCentral
27.
Zurück zum Zitat Hermosilla T, et al. Direct Thy-1/alphaVbeta3 integrin interaction mediates neuron to astrocyte communication. Biochim Biophys Acta. 2008;1783(6):1111–20.CrossRefPubMedPubMedCentral Hermosilla T, et al. Direct Thy-1/alphaVbeta3 integrin interaction mediates neuron to astrocyte communication. Biochim Biophys Acta. 2008;1783(6):1111–20.CrossRefPubMedPubMedCentral
28.
Zurück zum Zitat Leyton L, et al. Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites. Curr Biol. 2001;11(13):1028–38.CrossRefPubMed Leyton L, et al. Thy-1 binds to integrin beta(3) on astrocytes and triggers formation of focal contact sites. Curr Biol. 2001;11(13):1028–38.CrossRefPubMed
29.
Zurück zum Zitat Ellison JA, et al. Osteopontin and its integrin receptor alpha(v)beta3 are upregulated during formation of the glial scar after focal stroke. Stroke. 1998;29(8):1698–706. discussion 1707CrossRefPubMed Ellison JA, et al. Osteopontin and its integrin receptor alpha(v)beta3 are upregulated during formation of the glial scar after focal stroke. Stroke. 1998;29(8):1698–706. discussion 1707CrossRefPubMed
30.
Zurück zum Zitat Iseki K, et al. Increased syndecan expression by pleiotrophin and FGF receptor-expressing astrocytes in injured brain tissue. Glia. 2002;39(1):1–9.CrossRefPubMed Iseki K, et al. Increased syndecan expression by pleiotrophin and FGF receptor-expressing astrocytes in injured brain tissue. Glia. 2002;39(1):1–9.CrossRefPubMed
32.
Zurück zum Zitat Geevasinga N, et al. Pathophysiological and diagnostic implications of cortical dysfunction in ALS. Nat Rev Neurol. 2016;12(11):651–61.CrossRefPubMed Geevasinga N, et al. Pathophysiological and diagnostic implications of cortical dysfunction in ALS. Nat Rev Neurol. 2016;12(11):651–61.CrossRefPubMed
33.
Zurück zum Zitat van Zundert B, et al. Early pathogenesis in the adult-onset neurodegenerative disease amyotrophic lateral sclerosis. J Cell Biochem. 2012;113(11):3301–12.CrossRefPubMed van Zundert B, et al. Early pathogenesis in the adult-onset neurodegenerative disease amyotrophic lateral sclerosis. J Cell Biochem. 2012;113(11):3301–12.CrossRefPubMed
34.
Zurück zum Zitat Vucic S, Rothstein JD, Kiernan MC. Advances in treating amyotrophic lateral sclerosis: insights from pathophysiological studies. Trends Neurosci. 2014;37(8):433–42.CrossRefPubMed Vucic S, Rothstein JD, Kiernan MC. Advances in treating amyotrophic lateral sclerosis: insights from pathophysiological studies. Trends Neurosci. 2014;37(8):433–42.CrossRefPubMed
35.
Zurück zum Zitat Kushner PD, Stephenson DT, Wright S. Reactive astrogliosis is widespread in the subcortical white matter of amyotrophic lateral sclerosis brain. J Neuropath Exp Neurol. 1991;50(3):263–77.CrossRefPubMed Kushner PD, Stephenson DT, Wright S. Reactive astrogliosis is widespread in the subcortical white matter of amyotrophic lateral sclerosis brain. J Neuropath Exp Neurol. 1991;50(3):263–77.CrossRefPubMed
36.
37.
Zurück zum Zitat Hall ED, Oostveen JA, Gurney ME. Relationship of microglial and astrocytic activation to disease onset and progression in a transgenic model of familial ALS. Glia. 1998;23(2):249–56.CrossRefPubMed Hall ED, Oostveen JA, Gurney ME. Relationship of microglial and astrocytic activation to disease onset and progression in a transgenic model of familial ALS. Glia. 1998;23(2):249–56.CrossRefPubMed
38.
Zurück zum Zitat Di Giorgio FP, et al. Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model. Nat Neurosci. 2007;10:608–14.CrossRefPubMedPubMedCentral Di Giorgio FP, et al. Non-cell autonomous effect of glia on motor neurons in an embryonic stem cell-based ALS model. Nat Neurosci. 2007;10:608–14.CrossRefPubMedPubMedCentral
39.
Zurück zum Zitat Fritz E, et al. Mutant SOD1-expressing astrocytes release toxic factors that trigger motoneuron death by inducing hyperexcitability. J Neurophysiol. 2013;109(11):2803–14.CrossRefPubMedPubMedCentral Fritz E, et al. Mutant SOD1-expressing astrocytes release toxic factors that trigger motoneuron death by inducing hyperexcitability. J Neurophysiol. 2013;109(11):2803–14.CrossRefPubMedPubMedCentral
40.
Zurück zum Zitat Nagai M, et al. Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons. Nat Neurosci. 2007;10:615–22.CrossRefPubMedPubMedCentral Nagai M, et al. Astrocytes expressing ALS-linked mutated SOD1 release factors selectively toxic to motor neurons. Nat Neurosci. 2007;10:615–22.CrossRefPubMedPubMedCentral
41.
Zurück zum Zitat Rojas F, et al. Astrocytes expressing mutant SOD1 and TDP43 trigger motoneuron death that is mediated via sodium channels and nitroxidative stress. Front Cell Neurosci. 2014;8:24.CrossRefPubMedPubMedCentral Rojas F, et al. Astrocytes expressing mutant SOD1 and TDP43 trigger motoneuron death that is mediated via sodium channels and nitroxidative stress. Front Cell Neurosci. 2014;8:24.CrossRefPubMedPubMedCentral
42.
Zurück zum Zitat Rojas F, et al. Reactive oxygen species trigger motoneuron death in non-cell-autonomous models of ALS through activation of c-Abl signaling. Front Cell Neurosci. 2015;9:203.CrossRefPubMedPubMedCentral Rojas F, et al. Reactive oxygen species trigger motoneuron death in non-cell-autonomous models of ALS through activation of c-Abl signaling. Front Cell Neurosci. 2015;9:203.CrossRefPubMedPubMedCentral
43.
Zurück zum Zitat Apolloni S, et al. Actions of the antihistaminergic clemastine on presymptomatic SOD1-G93A mice ameliorate ALS disease progression. J Neuroinflammation. 2016;13(1):191.CrossRefPubMedPubMedCentral Apolloni S, et al. Actions of the antihistaminergic clemastine on presymptomatic SOD1-G93A mice ameliorate ALS disease progression. J Neuroinflammation. 2016;13(1):191.CrossRefPubMedPubMedCentral
44.
Zurück zum Zitat Gao B, Saba TM, Tsan MF. Role of alpha(v)beta(3)-integrin in TNF-alpha-induced endothelial cell migration. Am J Physiol Cell Physiol. 2002;283(4):C1196–205.CrossRefPubMed Gao B, Saba TM, Tsan MF. Role of alpha(v)beta(3)-integrin in TNF-alpha-induced endothelial cell migration. Am J Physiol Cell Physiol. 2002;283(4):C1196–205.CrossRefPubMed
45.
Zurück zum Zitat Shao N, et al. Interleukin-8 upregulates integrin beta3 expression and promotes estrogen receptor-negative breast cancer cell invasion by activating the PI3K/Akt/NF-kappaB pathway. Cancer Lett. 2015;364(2):165–72.CrossRefPubMed Shao N, et al. Interleukin-8 upregulates integrin beta3 expression and promotes estrogen receptor-negative breast cancer cell invasion by activating the PI3K/Akt/NF-kappaB pathway. Cancer Lett. 2015;364(2):165–72.CrossRefPubMed
46.
Zurück zum Zitat Foletti A, et al. Isolated integrin beta3 subunit cytoplasmic domains require membrane anchorage and the NPXY motif to recruit to adhesion complexes but do not discriminate between beta1- and beta3-positive complexes. Thromb Haemost. 2005;94(1):155–66.PubMed Foletti A, et al. Isolated integrin beta3 subunit cytoplasmic domains require membrane anchorage and the NPXY motif to recruit to adhesion complexes but do not discriminate between beta1- and beta3-positive complexes. Thromb Haemost. 2005;94(1):155–66.PubMed
47.
Zurück zum Zitat Fiore VF, et al. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211(1):173–90.CrossRefPubMedPubMedCentral Fiore VF, et al. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211(1):173–90.CrossRefPubMedPubMedCentral
48.
Zurück zum Zitat Schmidt M, et al. Thy-1/beta3 integrin interaction-induced apoptosis of dermal fibroblasts is mediated by up-regulation of FasL expression. J Invest Dermatol. 2016;136(2):526–9.CrossRefPubMed Schmidt M, et al. Thy-1/beta3 integrin interaction-induced apoptosis of dermal fibroblasts is mediated by up-regulation of FasL expression. J Invest Dermatol. 2016;136(2):526–9.CrossRefPubMed
49.
Zurück zum Zitat Saalbach A, et al. Interaction of human Thy-1 (CD 90) with the integrin alphavbeta3 (CD51/CD61): an important mechanism mediating melanoma cell adhesion to activated endothelium. Oncogene. 2005;24(29):4710–20.CrossRefPubMed Saalbach A, et al. Interaction of human Thy-1 (CD 90) with the integrin alphavbeta3 (CD51/CD61): an important mechanism mediating melanoma cell adhesion to activated endothelium. Oncogene. 2005;24(29):4710–20.CrossRefPubMed
50.
Zurück zum Zitat Barbeito LH, et al. A role for astrocytes in motor neuron loss in amyotrophic lateral sclerosis. Brain Res Brain Res Rev. 2004;47(1–3):263–74.CrossRefPubMed Barbeito LH, et al. A role for astrocytes in motor neuron loss in amyotrophic lateral sclerosis. Brain Res Brain Res Rev. 2004;47(1–3):263–74.CrossRefPubMed
51.
Zurück zum Zitat Maragakis NJ, Rothstein JD. Mechanisms of Disease: astrocytes in neurodegenerative disease. Nat Clin Pract Neurol. 2006;2(12):679–89.CrossRefPubMed Maragakis NJ, Rothstein JD. Mechanisms of Disease: astrocytes in neurodegenerative disease. Nat Clin Pract Neurol. 2006;2(12):679–89.CrossRefPubMed
52.
Zurück zum Zitat Renner NA, et al. Transient acidification and subsequent proinflammatory cytokine stimulation of astrocytes induce distinct activation phenotypes. J Cell Physiol. 2013;228(6):1284–94.CrossRefPubMedPubMedCentral Renner NA, et al. Transient acidification and subsequent proinflammatory cytokine stimulation of astrocytes induce distinct activation phenotypes. J Cell Physiol. 2013;228(6):1284–94.CrossRefPubMedPubMedCentral
53.
Zurück zum Zitat Herrera-Molina R, et al. Thy-1-interacting molecules and cellular signaling in cis and trans. Int Rev Cell Mol Biol. 2013;305:163–216.CrossRefPubMed Herrera-Molina R, et al. Thy-1-interacting molecules and cellular signaling in cis and trans. Int Rev Cell Mol Biol. 2013;305:163–216.CrossRefPubMed
54.
Zurück zum Zitat Leyton L, Hagood JS. Thy-1 modulates neurological cell-cell and cell-matrix interactions through multiple molecular interactions. Adv Neurobiol. 2014;8:3–20.CrossRefPubMed Leyton L, Hagood JS. Thy-1 modulates neurological cell-cell and cell-matrix interactions through multiple molecular interactions. Adv Neurobiol. 2014;8:3–20.CrossRefPubMed
55.
Zurück zum Zitat Aloisi F, et al. Astrocyte cultures from human embryonic brain: characterization and modulation of surface molecules by inflammatory cytokines. J Neurosci Res. 1992;32(4):494–506.CrossRefPubMed Aloisi F, et al. Astrocyte cultures from human embryonic brain: characterization and modulation of surface molecules by inflammatory cytokines. J Neurosci Res. 1992;32(4):494–506.CrossRefPubMed
56.
Zurück zum Zitat Shrikant P, et al. Regulation of intercellular adhesion molecule-1 gene expression by tumor necrosis factor-alpha, interleukin-1 beta, and interferon-gamma in astrocytes. J Neuroimmunol. 1994;51(2):209–20.CrossRefPubMed Shrikant P, et al. Regulation of intercellular adhesion molecule-1 gene expression by tumor necrosis factor-alpha, interleukin-1 beta, and interferon-gamma in astrocytes. J Neuroimmunol. 1994;51(2):209–20.CrossRefPubMed
57.
Zurück zum Zitat Hurwitz AA, et al. Tumor necrosis factor alpha induces adhesion molecule expression on human fetal astrocytes. J Exp Med. 1992;176(6):1631–6.CrossRefPubMed Hurwitz AA, et al. Tumor necrosis factor alpha induces adhesion molecule expression on human fetal astrocytes. J Exp Med. 1992;176(6):1631–6.CrossRefPubMed
58.
Zurück zum Zitat Cardenas A, et al. Signaling pathways involved in neuron-astrocyte adhesion and migration. Curr Mol Med. 2014;14(2):275–90.CrossRefPubMed Cardenas A, et al. Signaling pathways involved in neuron-astrocyte adhesion and migration. Curr Mol Med. 2014;14(2):275–90.CrossRefPubMed
59.
Zurück zum Zitat Froger N, et al. Inhibition of cytokine-induced connexin43 hemichannel activity in astrocytes is neuroprotective. Mol Cell Neurosci. 2010;45(1):37–46.CrossRefPubMed Froger N, et al. Inhibition of cytokine-induced connexin43 hemichannel activity in astrocytes is neuroprotective. Mol Cell Neurosci. 2010;45(1):37–46.CrossRefPubMed
60.
Zurück zum Zitat Chen G, et al. Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain. 2014;137(Pt 8):2193–209.CrossRefPubMedPubMedCentral Chen G, et al. Connexin-43 induces chemokine release from spinal cord astrocytes to maintain late-phase neuropathic pain in mice. Brain. 2014;137(Pt 8):2193–209.CrossRefPubMedPubMedCentral
61.
Zurück zum Zitat Xu P, et al. Extracellular ATP enhances radiation-induced brain injury through microglial activation and paracrine signaling via P2X7 receptor. Brain Behav Immun. 2015;50:87–100.CrossRefPubMed Xu P, et al. Extracellular ATP enhances radiation-induced brain injury through microglial activation and paracrine signaling via P2X7 receptor. Brain Behav Immun. 2015;50:87–100.CrossRefPubMed
62.
Zurück zum Zitat Monif M, et al. Interleukin-1beta has trophic effects in microglia and its release is mediated by P2X7R pore. J Neuroinflammation. 2016;13(1):173.CrossRefPubMedPubMedCentral Monif M, et al. Interleukin-1beta has trophic effects in microglia and its release is mediated by P2X7R pore. J Neuroinflammation. 2016;13(1):173.CrossRefPubMedPubMedCentral
63.
Zurück zum Zitat Monif M, et al. The P2X7 receptor drives microglial activation and proliferation: a trophic role for P2X7R pore. J Neurosci. 2009;29(12):3781–91.CrossRefPubMed Monif M, et al. The P2X7 receptor drives microglial activation and proliferation: a trophic role for P2X7R pore. J Neurosci. 2009;29(12):3781–91.CrossRefPubMed
64.
Zurück zum Zitat Shu Q, et al. Orexin-A promotes cell migration in cultured rat astrocytes via Ca2+-dependent PKCalpha and ERK1/2 signals. PLoS One. 2014;9(4):e95259.CrossRefPubMedPubMedCentral Shu Q, et al. Orexin-A promotes cell migration in cultured rat astrocytes via Ca2+-dependent PKCalpha and ERK1/2 signals. PLoS One. 2014;9(4):e95259.CrossRefPubMedPubMedCentral
65.
Zurück zum Zitat Furger KA, et al. Beta(3) integrin expression increases breast carcinoma cell responsiveness to the malignancy-enhancing effects of osteopontin. Mol Cancer Res. 2003;1(11):810–9.PubMed Furger KA, et al. Beta(3) integrin expression increases breast carcinoma cell responsiveness to the malignancy-enhancing effects of osteopontin. Mol Cancer Res. 2003;1(11):810–9.PubMed
66.
Zurück zum Zitat Gianni T, Leoni V, Campadelli-Fiume G. Type I interferon and NF-kappaB activation elicited by herpes simplex virus gH/gL via alphavbeta3 integrin in epithelial and neuronal cell lines. J Virol. 2013;87(24):13911–6.CrossRefPubMedPubMedCentral Gianni T, Leoni V, Campadelli-Fiume G. Type I interferon and NF-kappaB activation elicited by herpes simplex virus gH/gL via alphavbeta3 integrin in epithelial and neuronal cell lines. J Virol. 2013;87(24):13911–6.CrossRefPubMedPubMedCentral
67.
Zurück zum Zitat Cao L, et al. Osteopontin promotes a cancer stem cell-like phenotype in hepatocellular carcinoma cells via an integrin-NF-kappaB-HIF-1alpha pathway. Oncotarget. 2015;6(9):6627–40.CrossRefPubMedPubMedCentral Cao L, et al. Osteopontin promotes a cancer stem cell-like phenotype in hepatocellular carcinoma cells via an integrin-NF-kappaB-HIF-1alpha pathway. Oncotarget. 2015;6(9):6627–40.CrossRefPubMedPubMedCentral
68.
Zurück zum Zitat Courter DL, et al. Src kinase activity is required for integrin alphaVbeta3-mediated activation of nuclear factor-kappaB. J Biol Chem. 2005;280(13):12145–51.CrossRefPubMed Courter DL, et al. Src kinase activity is required for integrin alphaVbeta3-mediated activation of nuclear factor-kappaB. J Biol Chem. 2005;280(13):12145–51.CrossRefPubMed
69.
Zurück zum Zitat Okuyama E, et al. Molecular mechanisms of syndecan-4 upregulation by TNF-alpha in the endothelium-like EAhy926 cells. J Biochem. 2013;154(1):41–50.CrossRefPubMed Okuyama E, et al. Molecular mechanisms of syndecan-4 upregulation by TNF-alpha in the endothelium-like EAhy926 cells. J Biochem. 2013;154(1):41–50.CrossRefPubMed
70.
Zurück zum Zitat Balasubramaniyan V, et al. Importance of Connexin-43 based gap junction in cirrhosis and acute-on-chronic liver failure. J Hepatol. 2013;58(6):1194–200.CrossRefPubMed Balasubramaniyan V, et al. Importance of Connexin-43 based gap junction in cirrhosis and acute-on-chronic liver failure. J Hepatol. 2013;58(6):1194–200.CrossRefPubMed
71.
Zurück zum Zitat Alonso F, et al. An angiotensin II- and NF-kappaB-dependent mechanism increases connexin 43 in murine arteries targeted by renin-dependent hypertension. Cardiovasc Res. 2010;87(1):166–76.CrossRefPubMedPubMedCentral Alonso F, et al. An angiotensin II- and NF-kappaB-dependent mechanism increases connexin 43 in murine arteries targeted by renin-dependent hypertension. Cardiovasc Res. 2010;87(1):166–76.CrossRefPubMedPubMedCentral
72.
Zurück zum Zitat Ritchie CK, Giordano A, Khalili K. Integrin involvement in glioblastoma multiforme: possible regulation by NF-kappaB. J Cell Physiol. 2000;184(2):214–21.CrossRefPubMed Ritchie CK, Giordano A, Khalili K. Integrin involvement in glioblastoma multiforme: possible regulation by NF-kappaB. J Cell Physiol. 2000;184(2):214–21.CrossRefPubMed
Metadaten
Titel
αVβ3 Integrin regulates astrocyte reactivity
verfasst von
Raúl Lagos-Cabré
Alvaro Alvarez
Milene Kong
Francesca Burgos-Bravo
Areli Cárdenas
Edgardo Rojas-Mancilla
Ramón Pérez-Nuñez
Rodrigo Herrera-Molina
Fabiola Rojas
Pascal Schneider
Mario Herrera-Marschitz
Andrew F. G. Quest
Brigitte van Zundert
Lisette Leyton
Publikationsdatum
01.12.2017
Verlag
BioMed Central
Erschienen in
Journal of Neuroinflammation / Ausgabe 1/2017
Elektronische ISSN: 1742-2094
DOI
https://doi.org/10.1186/s12974-017-0968-5

Weitere Artikel der Ausgabe 1/2017

Journal of Neuroinflammation 1/2017 Zur Ausgabe

Neu in den Fachgebieten Neurologie und Psychiatrie

Akuter Schwindel: Wann lohnt sich eine MRT?

28.04.2024 Schwindel Nachrichten

Akuter Schwindel stellt oft eine diagnostische Herausforderung dar. Wie nützlich dabei eine MRT ist, hat eine Studie aus Finnland untersucht. Immerhin einer von sechs Patienten wurde mit akutem ischämischem Schlaganfall diagnostiziert.

Niedriger diastolischer Blutdruck erhöht Risiko für schwere kardiovaskuläre Komplikationen

25.04.2024 Hypotonie Nachrichten

Wenn unter einer medikamentösen Hochdrucktherapie der diastolische Blutdruck in den Keller geht, steigt das Risiko für schwere kardiovaskuläre Ereignisse: Darauf deutet eine Sekundäranalyse der SPRINT-Studie hin.

Frühe Alzheimertherapie lohnt sich

25.04.2024 AAN-Jahrestagung 2024 Nachrichten

Ist die Tau-Last noch gering, scheint der Vorteil von Lecanemab besonders groß zu sein. Und beginnen Erkrankte verzögert mit der Behandlung, erreichen sie nicht mehr die kognitive Leistung wie bei einem früheren Start. Darauf deuten neue Analysen der Phase-3-Studie Clarity AD.

Viel Bewegung in der Parkinsonforschung

25.04.2024 Parkinson-Krankheit Nachrichten

Neue arznei- und zellbasierte Ansätze, Frühdiagnose mit Bewegungssensoren, Rückenmarkstimulation gegen Gehblockaden – in der Parkinsonforschung tut sich einiges. Auf dem Deutschen Parkinsonkongress ging es auch viel um technische Innovationen.