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01.12.2009 | Original Article

A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro

verfasst von: Dunja M. Baston-Büst, Alexandra P. Hess, Jens Hirchenhain, Jürgen Krücken, Frank Wunderlich, Jan S. Krüssel, Ulrike K. Friebe-Hoffmann

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 6/2009

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Abstract

Purpose

Based on the reported tocolytic action of the hormone relaxin (RLX) in rodents, locally produced in reproductive tissues and the corpus luteum in mammals, the present study aimed to evaluate the influence of RLX on contraction-mediating cyclooxygenases-1 and -2 (COX) and the contractile prostaglandin PGE₂ in human myometrial and decidual cells. Primary cultured cells were obtained from uteri and placentas of term and preterm women undergoing elective caesarean section.

Methods

In vitro culture of primary myometrial and decidual cells, immunocytochemistry, reverse transcription and real-time PCR, Western blot, ELISA.

Results

We demonstrate for the first time an activating effect of RLX for human COX-1 and COX-2 in primary myometrial and decidual cells in vitro.

Conclusions

These effects might potentially contribute to birth-associated induction of contractions in vivo.

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Titel: A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro
verfasst von: Dunja M. Baston-Büst
Alexandra P. Hess
Jens Hirchenhain
Jürgen Krücken
Frank Wunderlich
Jan S. Krüssel
Ulrike K. Friebe-Hoffmann
Publikationsdatum: 01.12.2009
Verlag: Springer-Verlag
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 6/2009
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-009-1046-8

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A possible ambivalent role for relaxin in human myometrial and decidual cells in vitro