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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 8/2011

01.08.2011 | Technical Innovations

A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos

verfasst von: Katherine Gelber, Aileen N. Tamura, Vernadeth B. Alarcon, Yusuke Marikawa

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2011

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Abstract

Purpose

To assess the impact of embryonic stem cell culture medium (ESCM) on the pre- and post-implantation development of the mouse embryo, as a mammalian model, in comparison with the conventional culture medium, a potassium simplex optimized medium (KSOM).

Methods

Development in ESCM versus KSOM was compared in terms of embryo morphology, cleavage, cavitation, hatching, cell number, expression of TE and ICM transcription factors (Cdx2 and Oct4, respectively), implantation, and development in utero.

Results

An enriched medium like ESCM can be beneficial for in vitro embryo development when cultured from the 8-cell stage, as evidenced by promotion of blastocyst development with respect to cavity expansion, hatching, and cell division. Such benefits were not observed when embryos were cultured from the 2-cell stage.

Conclusions

ESCM may augment in vitro embryo development from the 8-cell stage. Using different culture media at different stages may be beneficial to achieve more effective human in vitro fertilization.
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Metadaten
Titel
A potential use of embryonic stem cell medium for the in vitro culture of preimplantation embryos
verfasst von
Katherine Gelber
Aileen N. Tamura
Vernadeth B. Alarcon
Yusuke Marikawa
Publikationsdatum
01.08.2011
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 8/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-011-9587-8

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